Human Recombinant Protein - CARP2 (1 - 363)

Name: CARP2 (1 - 363)
Price per aliquot (100µg): £125.00
Synonyms:
RFFL, RNF189, RNF34L, fring, rififylin
Species:
Human
Catalog Number (DU Number):
DU43464
Accession:
NM_001017368
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Ubiquitin chain assay with UBE1, Ubiquitin and E2 (UBE2D1, D2, D3 or UBE2N/UBDE2V1). Adding CARP2 will increase the amount if long isopeptide chains as monitored by immunoblotting against Ubiquitin.
Enzymatic Buffer:
25 mM HEPES pH 7.5, 1 mM DTT, 10 mM Mg-acetate, 0.2 mM ATP; Assay conditions:- 2 hours at 30 - 37 °C
Calculated molecular mass:
Mono-Isotopic Mass: 67,148.85
Average Mass: 67,192.87
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Storage Temperature:
–70 °C
Theoretical PI:
5.48
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – S363 of human CARP2. Residue M230 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 - 220.
Protease Cleavage:
rTev (ENLYFQG) residues 221 - 227
Cloning Sites:
BamH1 and Not1 sites of pGEX-6P-1

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