BDPIC can potentially induce proximity between any two intracellular proteins, each tagged with a bromoTAG (BD2(BRD4)-L387A) and dTAG (FKBP12-F36V), respectively. Proximity-induction has been seen experimentally as early as 5 minutes (Zhao et al, iScience, 2024) and at BDPIC concentrations ranging from 10-1000 nM (Zhao et al, 2024, PMID: 39081292; Brewer et al, 2024, PMID: 39104417).

We currently have a limited amount of BDPIC and can only share a small amount of BDPIC with researchers on a cost-recovery basis until it will be available commercially through a commercial partner.

BDPIC: 5 mg £500 (maximum of 2 orders)
BDPIC: 25 mg £2500 (maximum of 1 order; depending on availability)

Related orders (controls for BDPIC):

pcDNA5-FRT/TO-dTAG-NLuc (DU83490)
pcDNA5-FRT/TO-bromoTAG-HALO (83491)
TFEBdTAG/dTAG/bromoTAG/+PPP2CA knockin U2OS cells (Zhao et al, 2024; PMID: 39081292)
Anti-TFEB antibody (DA316)

Properties of BDPIC

Chemical equation: C67H84ClN6O15S
Molecular weight: 1280.95 g/mol
Observed [M + H]+ (m/z) (by MS): 1279.6
Stock dilution: 10 mM in DMSO; store at -20oC.

Key biological test data for BDPIC:

1. Bioluminescence Resonance Energy Transfer (BRET) assay:



Figure 1: Optimization of NanoBRET Luciferase assay for BDPIC-induced ternary complex formation between bromoTAG and dTAG. (A) A cartoon depicting the principles of energy transfer in a NanoBRET luciferase assay. First protein of interest (POI) is expressed as POI1-NanoLuc (NLuc) luciferase fusion. The second POI expressed as POI2-HALO fusion, for which a covalent cell-permeable fluorescent NanoBRET tracer is applied. When POI1 binds POI2 in cells, the tracer also becomes proximal to the NLuc luciferase and provided the proximity is optimal (i.e. a ternary complex has formed), it results in a bioluminescence resonance energy transfer (BRET) signal that can be measured. If BDPIC induces a ternary complex between bromoTAG and dTAG in live cells, using various combinations of NLuc and HALO tags on either protein should allow for determining BRET signals. (B) NLuc and HALO tags were attached to bromoTAG and dTAG in either N- or the C-termini in all combinations and expressed in cells. Luciferase and BRET signals were measured following treatment of cells with DMSO or BDPIC and BRET ratios plotted for each pair of constructs. Clear differences in BRET ratios induced by BDPIC compared to DMSO treatment are evident for NLuc-dTAG:bromoTAG-HALO, dTAG-NLuc:bromoTAG-HALO and bromoTAG-NLUC:dTAG-HALO combinations. (C) dTAG-NLuc and bromoTAG-HALO expressing cells were subjected to treatment with DMSO or the indicated doses of BDPIC as indicated for 24 h prior to lysis and BRET measurement, which are plotted.

2. Targeted dephoshorylation of TFEB-dTAG induced by BDPIC via recruitment of bromoTAG-PPP2CA (reproduced from Zhao et al, 2024, PMID: 39081292):