Human Recombinant Protein - CHIP (2 - 303) (N-terminal His)

Name: CHIP (2 - 303) (N-terminal His)
Price per aliquot (100µg): £125.00
Species:
Human
Catalog Number (DU Number):
DU3350
Accession:
NP_005852
Expression:
bacteria
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+ -NTA agarose
Enzymatic Assay Format:
Ubiquitin assay
Calculated molecular mass:
Mono-Isotopic Mass: 38,244.77
Average Mass: 38, 268.91
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Storage Temperature:
-70 °C
Theoretical PI:
6.16
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids K2 – Y303 (end) of CHIP. Residue K35 of the fusion protein is equilivalent to K2 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
Thrombin (LVPRGS) residues 14 - 19
Cloning Sites:
BamH1 and Not1 site of pET28a

Do you need any help? Please get in touch and we’ll be happy to lend a hand.