Human Recombinant Protein - CHIP (2 - 303)

Name: CHIP (2 - 303)
Price per aliquot (100µg): £125.00
Species:
Human
Catalog Number (DU Number):
DU1993
Accession:
NP_005852
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Ubiquitin assay
Calculated molecular mass:
Mono-Isotopic Mass: 61,509.84
Average Mass: 61,549.18
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C
Theoretical PI:
5.66
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids K2 – Y303 (end) of CHIP. Residue K232 of the fusion protein is equilivalent to K2 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
BamH1 and Not1 site of pGex6P-1

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