cDNA Clone USP35 Synthetic

• Plasmid: pET28a 6His-Halo.TEV USP35
• Parent Plasmid: pET28a 6His Halo TEV
• Expressed: 6His-Halo.TEV USP35
• Genbank: Q9P2H5
• Tag: 6His
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGGACAAGATCTTGGAGGCGGTGGTGACGTCGTCATACCCGGT
CAGCGTGAAGCAGGGGCTGGTTCGGCGCGTGCTGGAGGCGGCGCGGCAGC
CGCTGGAGCGTGAGCAGTGCCTGGCGCTGCTGGCGCTGGGCGCGCGCCTC
TACGTGGGCGGCGCGGAGGAGCTGCCGCGCCGCGTGGGCTGCCAGCTGCT
GCACGTGGCCGGCCGCCACCACCCCGACGTCTTCGCCGAGTTCTTCAGCG
CGCGTCGCGTGCTGCGCCTGCTGCAGGGTGGCGCCGGCCCCCCGGGCCCC
CGCGCGCTCGCCTGCGTGCAGCTGGGTCTGCAGCTGCTGCCCGAGGGGCC
TGCGGCCGACGAGGTGTTCGCGCTGCTGCGGCGCGAGGTGCTGCGCACCG
TGTGCGAGCGCCCGGGCCCCGCGGCCTGCGCGCAGGTGGCACGGCTGCTG
GCTCGCCACCCGCGCTGTGTGCCCGACGGACCCCACCGCCTGCTCTTCTG
CCAGCAGCTGGTGCGTTGCCTCGGCCGCTTCCGCTGCCCAGCCGAAGGCG
AGGAGGGCGCCGTGGAGTTCCTAGAGCAGGCCCAGCAGGTGAGCGGGCTC
CTGGCGCAGCTGTGGCGCGCACAGCCCGCCGCCATCCTGCCCTGCCTCAA
AGAGCTGTTCGCAGTCATCTCCTGCGCAGAGGAGGAGCCACCATCTAGCG
CCCTGGCCAGCGTGGTCCAGCACCTCCCATTGGAGCTCATGGATGGTGTT
GTCCGGAACCTCAGCAATGATGACAGTGTGACAGACTCGCAGATGCTGAC
TGCCATTAGCAGGATGATTGACTGGGTGTCCTGGCCCCTGGGGAAGAATA
TTGACAAGTGGATCATTGCACTGCTGAAGGGCCTGGCTGCTGTTAAGAAG
TTCAGCATCTTGATCGAGGTTTCGCTCACCAAAATTGAGAAGGTTTTCTC
TAAGCTGCTGTACCCCATCGTCCGGGGAGCTGCCTTGTCTGTGCTCAAGT
ACATGCTCCTGACCTTCCAGCACTCCCACGAAGCCTTCCACCTGCTCCTC
CCTCACATCCCCCCCATGGTGGCCTCTCTGGTCAAGGAGGACTCGAACTC
GGGGACCAGCTGCCTGGAGCAGCTGGCGGAGCTGGTCCACTGCATGGTGT
TCCGGTTCCCGGGCTTCCCGGATCTGTATGAGCCTGTCATGGAGGCCATC
AAGGACCTCCATGTTCCCAATGAGGACCGCATCAAGCAGCTGCTGGGGCA
GGATGCCTGGACTTCGCAGAAGAGCGAGCTGGCGGGTTTCTATCCCCGGC
TCATGGCCAAGTCAGACACGGGCAAGATTGGTCTCATCAACCTGGGCAAC
ACATGCTATGTCAACAGCATCCTTCAGGCCTTATTCATGGCGTCTGACTT
CAGACATTGTGTGCTCCGCTTGACTGAGAACAACTCACAGCCCCTGATGA
CCAAGCTGCAGTGGCTCTTTGGCTTCCTAGAACACAGCCAGCGGCCTGCC
ATTTCCCCAGAGAACTTCCTCTCCGCATCCTGGACGCCCTGGTTCAGCCC
TGGCACCCAGCAGGACTGCTCGGAGTATCTGAAGTACCTGCTGGATCGGC
TGCACGAAGAGGAGAAAACGGGCACAAGGATCTGCCAGAAACTCAAGCAG
TCCAGCTCGCCCTCTCCGCCCGAGGAGCCCCCGGCCCCAAGTTCAACCTC
TGTGGAAAAAATGTTTGGAGGCAAGATAGTGACTCGGATCTGCTGTCTCT
GCTGCCTCAACGTCTCCTCCCGGGAGGAGGCCTTCACGGACCTCTCTCTC
GCCTTCCCTCCTCCTGAGCGCTGTCGCCGCCGCCGCCTGGGCTCTGTGAT
GCGCCCCACAGAAGACATCACAGCCCGGGAGTTGCCCCCACCAACCAGTG
CACAGGGGCCAGGCAGGGTGGGTCCTCGGAGGCAAAGGAAACACTGCATC
ACAGAGGACACCCCCCCCACCAGCCTGTACATCGAAGGCCTGGACTCCAA
GGAAGCTGGTGGGCAGAGCAGTCAGGAGGAAAGGATAGAGAGGGAGGAAG
AAGGGAAGGAGGAGAGAACGGAGAAGGAAGAAGTGGGGGAGGAGGAGGAA
AGCACCAGAGGGGAAGGAGAGAGGGAGAAAGAGGAGGAGGTGGAAGAGGA
AGAAGAGAAGGTGGAGAAGGAGACAGAAAAGGAGGCTGAGCAGGAAAAGG
AAGAAGACAGCCTGGGAGCGGGGACCCACCCGGATGCTGCCATCCCCTCC
GGGGAGCGGACATGTGGCTCTGAGGGCTCCCGCTCCGTCCTGGACCTGGT
TAACTACTTCCTGTCCCCCGAGAAGCTGACAGCAGAAAACCGCTACTACT
GCGAGTCGTGTGCCTCCCTGCAGGATGCCGAGAAGGTGGTGGAGCTGAGC
CAAGGGCCGTGCTACCTCATCCTCACACTGCTGCGCTTCTCTTTCGACCT
GCGCACCATGCGGCGCCGCAAGATCCTGGATGACGTCTCCATCCCCCTGC
TGCTCCGCCTGCCACTGGCTGGTGGCCGTGGCCAGGCCTATGACCTCTGC
AGTGTGGTGGTGCACTCTGGAGTGTCTTCGGAGAGTGGTCACTACTACTG
CTATGCCCGTGAGGGCGCTGCCCGCCCTGCCGCTTCTCTGGGAACTGCCG
ATAGGCCAGAGCCCGAGAACCAGTGGTACCTGTTCAATGACACTCGGGTG
TCCTTCTCTTCCTTCGAATCTGTCAGCAACGTCACCTCCTTCTTCCCTAA
GGACACAGCCTATGTGCTGTTTTACCGGCAGCGGCCCAGGGAGGGGCCCG
AGGCTGAGTTGGGCTCTTCTAGAGTCCGGACAGAGCCCACCCTGCACAAG
GACTTGATGGAAGCCATTTCCAAAGACAACATCCTTTACCTACAGGAGCA
GGAGAAGGAGGCCCGGAGCAGGGCGGCCTACATCTCTGCACTCCCCACAT
CTCCGCACTGGGGGAGGGGCTTTGATGAAGACAAGGATGAGGATGAAGGC
TCTCCAGGGGGCTGCAATCCTGCAGGTGGCAATGGTGGTGACTTCCACAG
ACTGGTCTTCTAAGCGGCCGC

Amino Acid Sequence

MHHHHHHMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPT
SSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIE
ALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPE
FARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPF
LNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTP
GVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTL
EISGENLYFQGGSMDKILEAVVTSSYPVSVKQGLVRRVLEAARQPLEREQ
CLALLALGARLYVGGAEELPRRVGCQLLHVAGRHHPDVFAEFFSARRVLR
LLQGGAGPPGPRALACVQLGLQLLPEGPAADEVFALLRREVLRTVCERPG
PAACAQVARLLARHPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVE
FLEQAQQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVV
QHLPLELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWII
ALLKGLAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTF
QHSHEAFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGF
PDLYEPVMEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSD
TGKIGLINLGNTCYVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWL
FGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEK
TGTRICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVS
SREEAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGR
VGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEER
TEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLG
AGTHPDAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCAS
LQDAEKVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPL
AGGRGQAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPE
NQWYLFNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGS
SRVRTEPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGR
GFDEDKDEDEGSPGGCNPAGGNGGDFHRLVF*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU46086
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 45630