cDNA Clone FLAG-GG-Ubiquitin [K all R]

• Plasmid: pGEX6P-1-FLAG-2Gly-Ubiquitin KallR
• Parent Plasmid: pGEX6P-1
• Expressed: GST-RV3C-FLAG-Gly-Gly-Ubiquitin [K all R]
• Genbank: P62987
• Tag: GST
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCGCCCTTGCAGATCTGCCACCATGGACTACAAGGACGACGATGAC
AAGGGAGGCATGCAAATCTTCGTGAGGACCCTGACTGGTAGGACCATCAC
TCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAGGATCC
AAGACAGGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGG
AGACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGA
GTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSRIRPCRSATMDYKDDDDKG
GMQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGRQ
LEDGRTLSDYNIQRESTLHLVLRLRGG**

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29879
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Gly-Gly spacer inserted and ubiquitin native methionine restored
• Low Temp: No