cDNA Clone FOXO3A

• Plasmid: pEGFP-C1-FKHR L1
• Parent Plasmid: pEGFPC1
• Expressed: GFP-FKHR L1
• Genbank: NP_001446
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGGCAGAGGCACC
GGCTTCCCCGGCCCCGCTCTCTCCGCTCGAAGTGGAGCTGGACCCGGAGT
TCGAGCCCCAGAGCCGTCCGCGATCCTGTACGTGGCCCCTGCAAAGGCCG
GAGCTCCAAGCGAGCCCTGCCAAGCCCTCGGGGGAGACGGCCGCTGACTC
CATGATCCCCGAGGAGGAGGACGATGAAGACGACGAGGACGGCGGGGGAC
GGGCCGGCTCGGCCATGGCGATCGGCGGCGGCGGCGGGAGCGGCACGCTG
GGCTCCGGGCTGCTCCTTGAGGACTCGGCCCGGGTGCTGGCACCCGGAGG
GCAAGACCCCGGGTCTGGGCCAGCCACCGCGGCGGGCGGGCTGAGCGGGG
GTACACAGGCGCTGCTGCAGCCTCAGCAACCGCTGCCACCGCCGCAGCCG
GGGGCGGCTGGGGGCTCCGGGCAGCCGAGGAAATGTTCGTCGCGGCGGAA
CGCCTGGGGAAACCTGTCCTACGCGGACCTGATCACCCGCGCCATCGAGA
GCTCCCCGGACAAACGGCTCACTCTGTCCCAGATCTACGAGTGGATGGTG
CGTTGCGTGCCCTACTTCAAGGATAAGGGCGACAGCAACAGCTCTGCCGG
CTGGAAGAACTCCATCCGGCACAACCTGTCACTGCATAGTCGATTCATGC
GGGTCCAGAATGAGGGAACTGGCAAGAGCTCTTGGTGGATCATCAACCCT
GATGGGGGGAAGAGCGGAAAAGCCCCCCGGCGGCGGGCTGTCTCCATGGA
CAATAGCAACAAGTATACCAAGAGCCGTGGCCGCGCAGCCAAGAAGAAGG
CAGCCCTGCAGACAGCCCCCGAATCAGCTGACGACAGTCCCTCCCAGCTC
TCCAAGTGGCCTGGCAGCCCCACGTCACGCAGCAGTGATGAGCTGGATGC
GTGGACGGACTTCCGTTCACGCACCAATTCTAACGCCAGCACAGTCAGTG
GCCGCCTGTCGCCCATCATGGCAAGCACAGAGTTGGATGAAGTCCAGGAC
GATGATGCGCCTCTCTCGCCCATGCTCTACAGCAGCTCAGCCAGCCTGTC
ACCTTCAGTAAGCAAGCCGTGCACGGTGGAACTGCCACGGCTGACTGATA
TGGCAGGCACCATGAATCTGAATGATGGGCTGACTGAAAACCTCATGGAC
GACCTGCTGGATAACATCACGCTCCCGCCATCCCAGCCATCGCCCACTGG
GGGACTCATGCAGCGGAGCTCTAGCTTCTCGTATACCACCAAGGGCTCGG
GCCTGGGCTCCCCAACCAGCTCCTTTAACAGCACGGTGTTCGGACCTTCA
TCTCTGAACTCCCTACGCCAGTCTCCCATGCAGACCATCCAAGAGAACAA
GCCAGCTACCTTCTCTTCCATGTCACACTATGGTAACCAGACACTCCAGG
ACCTGCTCACTTCGGACTCACTTAGCCACAGCGATGTCATGATGACACAG
TCGGACCCCTTGATGTCTCAGGCCAGCACCGCTGTGTCTGCCCAGAATTC
CCGCCGGAACGTGATGCTTCGCAATGATCCGATGATGTCCTTTGCTGCCC
AGCCTAACCAGGGAAGTTTGGTCAATCAGAACTTGCTCCACCACCAGCAC
CAAACCCAGGGCGCTCTTGGTGGCAGCCGTGCCTTGTCGAATTCTGTCAG
CAACATGGGCTTGAGTGAGTCCAGCAGCCTTGGGTCAGCCAAACACCAGC
AGCAGTCTCCTGTCAGCCAGTCTATGCAAACCCTCTCGGACTCTCTCTCA
GGCTCCTCCTTGTACTCAACTAGTGCAAACCTGCCCGTCATGGGCCATGA
GAAGTTCCCCAGCGACTTGGACCTGGACATGTTCAATGGGAGCTTGGAAT
GTGACATGGAGTCCATTATCCGTAGTGAACTCATGGATGCTGATGGGTTG
GATTTTAACTTTGATTCCCTCATCTCCACACAGAATGTTGTTGGTTTGAA
CGTGGGGAACTTCACTGGTGCTAAGCAGGCCTCATCTCAGAGCTGGGTGC
CAGGCTGAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSATMDYKDDDDKAEAPASPAPLSPLEVELDPEFEPQSRPR
SCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAI
GGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQP
QQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLT
LSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTG
KSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPE
SADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMA
STELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLN
DGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFSYTTKGSGLGSPTSS
FNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSL
SHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLV
NQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQS
MQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIR
SELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU3631
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/FLAG tag for mammalian expression.