cDNA Clone FOXO3A

• Plasmid: pCMV5-FLAG FKHR L1 S284A
• Parent Plasmid: pCMV5
• Expressed: FLAG-FKHR L1 S284A
• Genbank: NP_001446
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGGCAGAGGCACC
GGCTTCCCCGGCCCCGCTCTCTCCGCTCGAAGTGGAGCTGGACCCGGAGT
TCGAGCCCCAGAGCCGTCCGCGATCCTGTACGTGGCCCCTGCAAAGGCCG
GAGCTCCAAGCGAGCCCTGCCAAGCCCTCGGGGGAGACGGCCGCTGACTC
CATGATCCCCGAGGAGGAGGACGATGAAGACGACGAGGACGGCGGGGGAC
GGGCCGGCTCGGCCATGGCGATCGGCGGCGGCGGCGGGAGCGGCACGCTG
GGCTCCGGGCTGCTCCTTGAGGACTCGGCCCGGGTGCTGGCACCCGGAGG
GCAAGACCCCGGGTCTGGGCCAGCCACCGCGGCGGGCGGGCTGAGCGGGG
GTACACAGGCGCTGCTGCAGCCTCAGCAACCGCTGCCACCGCCGCAGCCG
GGGGCGGCTGGGGGCTCCGGGCAGCCGAGGAAATGTTCGTCGCGGCGGAA
CGCCTGGGGAAACCTGTCCTACGCGGACCTGATCACCCGCGCCATCGAGA
GCTCCCCGGACAAACGGCTCACTCTGTCCCAGATCTACGAGTGGATGGTG
CGTTGCGTGCCCTACTTCAAGGATAAGGGCGACAGCAACAGCTCTGCCGG
CTGGAAGAACTCCATCCGGCACAACCTGTCACTGCATAGTCGATTCATGC
GGGTCCAGAATGAGGGAACTGGCAAGAGCTCTTGGTGGATCATCAACCCT
GATGGGGGGAAGAGCGGAAAAGCCCCCCGGCGGCGGGCTGTCTCCATGGA
CAATAGCAACAAGTATACCAAGAGCCGTGGCCGCGCAGCCAAGAAGAAGG
CAGCCCTGCAGACAGCCCCCGAATCAGCTGACGACGCTCCCTCCCAGCTC
TCCAAGTGGCCTGGCAGCCCCACGTCACGCAGCAGTGATGAGCTGGATGC
GTGGACGGACTTCCGTTCACGCACCAATTCTAACGCCAGCACAGTCAGTG
GCCGCCTGTCGCCCATCATGGCAAGCACAGAGTTGGATGAAGTCCAGGAC
GATGATGCGCCTCTCTCGCCCATGCTCTACAGCAGCTCAGCCAGCCTGTC
ACCTTCAGTAAGCAAGCCGTGCACGGTGGAACTGCCACGGCTGACTGATA
TGGCAGGCACCATGAATCTGAATGATGGGCTGACTGAAAACCTCATGGAC
GACCTGCTGGATAACATCACGCTCCCGCCATCCCAGCCATCGCCCACTGG
GGGACTCATGCAGCGGAGCTCTAGCTTCCCGTATACCACCAAGGGCTCGG
GCCTGGGCTCCCCAACCAGCTCCTTTAACAGCACGGTGTTCGGACCTTCA
TCTCTGAACTCCCTACGCCAGTCTCCCATGCAGACCATCCAAGAGAACAA
GCCAGCTACCTTCTCTTCCATGTCACACTATGGTAACCAGACACTCCAGG
ACCTGCTCACTTCGGACTCACTTAGCCACAGCGATGTCATGATGACACAG
TCGGACCCCTTGATGTCTCAGGCCAGCACCGCTGTGTCTGCCCAGAATTC
CCGCCGGAACGTGATGCTTCGCAATGATCCGATGATGTCCTTTGCTGCCC
AGCCTAACCAGGGAAGTTTGGTCAATCAGAACTTGCTCCACCACCAGCAC
CAAACCCAGGGCGCTCTTGGTGGCAGCCGTGCCTTGTCGAATTCTGTCAG
CAACATGGGCTTGAGTGAGTCCAGCAGCCTTGGGTCAGCCAAACACCAGC
AGCAGTCTCCTGTCAGCCAGTCTATGCAAACCCTCTCGGACTCTCTCTCA
GGCTCCTCCTTGTACTCAACTAGTGCAAACCTGCCCGTCATGGGCCATGA
GAAGTTCCCCAGCGACTTGGACCTGGACATGTTCAATGGGAGCTTGGAAT
GTGACATGGAGTCCATTATCCGTAGTGAACTCATGGATGCTGATGGGTTG
GATTTTAACTTTGATTCCCTCATCTCCACACAGAATGTTGTTGGTTTGAA
CGTGGGGAACTTCACTGGTGCTAAGCAGGCCTCATCTCAGAGCTGGGTGC
CAGGCTGAGGATCC

Amino Acid Sequence

MDYKDDDDKAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQA
SPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGL
LLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAG
GSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVP
YFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGK
SGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDAPSQLSKWP
GSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAP
LSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLD
NITLPPSQPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNS
LRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPL
MSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQG
ALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSL
YSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNF
DSLISTQNVVGLNVGNFTGAKQASSQSWVPG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3200
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal FLAG tag for mammalian expression.