cDNA Clone FOXO3A

• Plasmid: ECFP-C1 FKHR-L1 S344A
• Parent Plasmid: pECFPC1
• Expressed: CFP-FKHR-L1 S344A
• Genbank: NP_001446
• Tag: CFP
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGGCAGAGGCACC
GGCTTCCCCGGCCCCGCTCTCTCCGCTCGAAGTGGAGCTGGACCCGGAGT
TCGAGCCCCAGAGCCGTCCGCGATCCTGTACGTGGCCCCTGCAAAGGCCG
GAGCTCCAAGCGAGCCCTGCCAAGCCCTCGGGGGAGACGGCCGCTGACTC
CATGATCCCCGAGGAGGAGGACGATGAAGACGACGAGGACGGCGGGGGAC
GGGCCGGCTCGGCCATGGCGATCGGCGGCGGCGGCGGGAGCGGCACGCTG
GGCTCCGGGCTGCTCCTTGAGGACTCGGCCCGGGTGCTGGCACCCGGAGG
GCAAGACCCCGGGTCTGGGCCAGCCACCGCGGCGGGCGGGCTGAGCGGGG
GTACACAGGCGCTGCTGCAGCCTCAGCAACCGCTGCCACCGCCGCAGCCG
GGGGCGGCTGGGGGCTCCGGGCAGCCGAGGAAATGTTCGTCGCGGCGGAA
CGCCTGGGGAAACCTGTCCTACGCGGACCTGATCACCCGCGCCATCGAGA
GCTCCCCGGACAAACGGCTCACTCTGTCCCAGATCTACGAGTGGATGGTG
CGTTGCGTGCCCTACTTCAAGGATAAGGGCGACAGCAACAGCTCTGCCGG
CTGGAAGAACTCCATCCGGCACAACCTGTCACTGCATAGTCGATTCATGC
GGGTCCAGAATGAGGGAACTGGCAAGAGCTCTTGGTGGATCATCAACCCT
GATGGGGGGAAGAGCGGAAAAGCCCCCCGGCGGCGGGCTGTCTCCATGGA
CAATAGCAACAAGTATACCAAGAGCCGTGGCCGCGCAGCCAAGAAGAAGG
CAGCCCTGCAGACAGCCCCCGAATCAGCTGACGACAGTCCCTCCCAGCTC
TCCAAGTGGCCTGGCAGCCCCACGTCACGCAGCAGTGATGAGCTGGATGC
GTGGACGGACTTCCGTTCACGCACCAATTCTAACGCCAGCACAGTCAGTG
GCCGCCTGTCGCCCATCATGGCAAGCACAGAGTTGGATGAAGTCCAGGAC
GATGATGCGCCTCTCGCGCCCATGCTCTACAGCAGCTCAGCCAGCCTGTC
ACCTTCAGTAAGCAAGCCGTGCACGGTGGAACTGCCACGGCTGACTGATA
TGGCAGGCACCATGAATCTGAATGATGGGCTGACTGAAAACCTCATGGAC
GACCTGCTGGATAACATCACGCTCCCGCCATCCCAGCCATCGCCCACTGG
GGGACTCATGCAGCGGAGCTCTAGCTTCTCGTATACCACCAAGGGCTCGG
GCCTGGGCTCCCCAACCAGCTCCTTTAACAGCACGGTGTTCGGACCTTCA
TCTCTGAACTCCCTACGCCAGTCTCCCATGCAGACCATCCAAGAGAACAA
GCCAGCTACCTTCTCTTCCATGTCACACTATGGTAACCAGACACTCCAGG
ACCTGCTCACTTCGGACTCACTTAGCCACAGCGATGTCATGATGACACAG
TCGGACCCCTTGATGTCTCAGGCCAGCACCGCTGTGTCTGCCCAGAATTC
CCGCCGGAACGTGATGCTTCGCAATGATCCGATGATGTCCTTTGCTGCCC
AGCCTAACCAGGGAAGTTTGGTCAATCAGAACTTGCTCCACCACCAGCAC
CAAACCCAGGGCGCTCTTGGTGGCAGCCGTGCCTTGTCGAATTCTGTCAG
CAACATGGGCTTGAGTGAGTCCAGCAGCCTTGGGTCAGCCAAACACCAGC
AGCAGTCTCCTGTCAGCCAGTCTATGCAAACCCTCTCGGACTCTCTCTCA
GGCTCCTCCTTGTACTCAACTAGTGCAAACCTGCCCGTCATGGGCCATGA
GAAGTTCCCCAGCGACTTGGACCTGGACATGTTCAATGGGAGCTTGGAAT
GTGACATGGAGTCCATTATCCGTAGTGAACTCATGGATGCTGATGGGTTG
GATTTTAACTTTGATTCCCTCATCTCCACACAGAATGTTGTTGGTTTGAA
CGTGGGGAACTTCACTGGTGCTAAGCAGGCCTCATCTCAGAGCTGGGTGC
CAGGCTGAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHN
VYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSTGSATMDYKDDDDKAEAPASPAPLSPLEVELDPEFEPQS
RPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSA
MAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQAL
LQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDK
RLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNE
GTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQT
APESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSP
IMASTELDEVQDDDAPLAPMLYSSSASLSPSVSKPCTVELPRLTDMAGTM
NLNDGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFSYTTKGSGLGSP
TSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTS
DSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQG
SLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPV
SQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMES
IIRSELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3151
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal CFP/FLAG tag for mammalian expression.