cDNA Clone FOXO1A

• Plasmid: pCMV5 FKHR S256A S319A
• Parent Plasmid: pEBG2T
• Expressed: HA-F FKHR S256A S319A
• Genbank: NM_002015
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGACGTCCCAGACTACGCTGCCGAGGC
GCCTCAGGTGGTGGAGATCGACCCGGACTTCGAGCCGCTGCCCCGGCCGC
GCTCGTGCACCTGGCCGCTGCCCAGGCCGGAGTTTAGCCAGTCCAACTCG
GCCACCTCCAGCCCGGCGCCGTCGGGCAGCGCGGCTGCCAACCCCGACGC
CGCGGCGGGCCTGCCCTCGGCCTCGGCTGCCGCTGTCAGCGCCGACTTCA
TGAGCAACCTGAGCTTGCTGGAGGAGAGCGAGGACTTCCCGCAGGCGCCC
GGCTCCGTGGCGGCGGCGGTGGCGGCGGCGGCCGCCGCGGCCGCCACCGG
GGGGCTGTGCGGGGACTTCCAGGGCCCGGAGGCGGGCTGCCTGCACCCAG
CGCCACCGCAGCCCCCGCCGCCCGGGCCGCTGTCGCAGCACCCGCCGGTG
CCCCCCGCCGCCGCTGGGCCGCTCGCGGGGCAGCCGCGCAAGAGCAGCTC
GTCCCGCCGCAACGCGTGGGGCAACCTGTCCTACGCCGACCTCATCACCA
AGGCCATCGAGAGCTCGGCGGAGAAGCGGCTCACGCTGTCGCAGATCTAC
GAGTGGATGGTCAAGAGCGTGCCCTACTTCAAGGATAAGGGTGACAGCAA
CAGCTCGGCGGGCTGGAAGAATTCAATTCGTCATAATCTGTCCCTACACA
GCAAGTTCATTCGTGTGCAGAATGAAGGAACTGGAAAAAGTTCTTGGTGG
ATGCTCAATCCAGAGGGTGGCAAGAGCGGGAAATCTCCTAGGAGAAGAGC
TGCAGCCATGGACAACAACAGTAAATTTGCTAAGAGCCGAAGCCGAGCTG
CCAAGAAGAAAGCATCTCTCCAGTCTGGCCAGGAGGGTGCTGGGGACAGC
CCTGGATCACAGTTTTCCAAATGGCCTGCAAGCCCTGGCTCTCACAGCAA
TGATGACTTTGATAACTGGAGTACATTTCGCCCTCGAACTAGCGCAAATG
CTAGTACTATTAGTGGGAGACTCTCACCCATTATGACCGAACAGGATGAT
CTTGGAGAAGGGGATGTGCATTCTATGGTGTACCCGCCATCTGCCGCAAA
GATGGCCTCTACTTTACCCAGTCTGTCTGAGATAAGCAATCCCGAAAACA
TGGAAAATCTTTTGGATAATCTCAACCTTCTCTCATCACCAACATCATTA
ACTGTTTCGACCCAGTCCTCACCTGGCACCATGATGCAGCAGACGCCGTG
CTACTCGTTTGCGCCACCAAACACCAGTTTGAATTCACCCAGCCCAAACT
ACCAAAAATATACATATGGCCAATCCAGCATGAGCCCTTTGCCCCAGATG
CCTATACAAACACTTCAGGACAATAAGTCGAGTTATGGAGGTATGAGTCA
GTATAACTGTGCGCCTGGACTCTTGAAGGAGTTGCTGACTTCTGACTCTC
CTCCCCATAATGACATTATGACACCAGTTGATCCTGGGGTAGCCCAGCCC
AACAGCCGGGTTCTGGGCCAGAACGTCATGATGGGCCCTAATTCGGTCAT
GTCAACCTATGGCAGCCAGGCATCTCATAACAAAATGATGAATCCCAGCT
CCCATACCCACCCTGGACATGCTCAGCAGACATCTGCAGTTAACGGGCGT
CCCCTGCCCCACACGGTAAGCACCATGCCCCACACCTCGGGTATGAACCG
CCTGACCCAAGTGAAGACACCTGTACAAGTGCCTCTGCCCCACCCCATGC
AGATGAGTGCCCTGGGGGGCTACTCCTCCGTGAGCAGCTGCAATGGCTAT
GGCAGAATGGGCCTTCTCCACCAGGAGAAGCTCCCAAGTGACTTGGATGG
CATGTTCATTGAGCGCTTAGACTGTGACATGGAATCCATCATTCGGAATG
ACCTCATGGATGGAGATACATTGGATTTTAACTTTGACAATGTGTTGCCC
AACCAAAGCTTCCCACACAGTGTCAAGACAACGACACATAGCTGGGTGTC
AGGCTGAGGGTTAGTGAGCAGGTTACACTTAAAAGTACTTCAGATTGTCT
GACAGCAGGAACTGAGAGAAGCAGTCCAAAGATGTCTTTCACCAACTCCC
TTTTAGTTTTCTCGGTTAAAAAAAAAAAAAAAAAAAAGGAATTCGATATC
AAGCTTATCGATACCGTCGACCCGGGCGGCCGCAAAGTTCCCTTTAGTGA
GGGTTAATGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACA
AACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTG
ATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTAAGCCGAATTCTGCAGATATCCATCACACTGGCGGCCGCT
CGAGCATGCATCTAGAGGATCC

Amino Acid Sequence

MYPYDVPDYAAEAPQVVEIDPDFEPLPRPRSCTWPLPRPEFSQSNSATSS
PAPSGSAAANPDAAAGLPSASAAAVSADFMSNLSLLEESEDFPQAPGSVA
AAVAAAAAAAATGGLCGDFQGPEAGCLHPAPPQPPPPGPLSQHPPVPPAA
AGPLAGQPRKSSSSRRNAWGNLSYADLITKAIESSAEKRLTLSQIYEWMV
KSVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFIRVQNEGTGKSSWWMLNP
EGGKSGKSPRRRAAAMDNNSKFAKSRSRAAKKKASLQSGQEGAGDSPGSQ
FSKWPASPGSHSNDDFDNWSTFRPRTSANASTISGRLSPIMTEQDDLGEG
DVHSMVYPPSAAKMASTLPSLSEISNPENMENLLDNLNLLSSPTSLTVST
QSSPGTMMQQTPCYSFAPPNTSLNSPSPNYQKYTYGQSSMSPLPQMPIQT
LQDNKSSYGGMSQYNCAPGLLKELLTSDSPPHNDIMTPVDPGVAQPNSRV
LGQNVMMGPNSVMSTYGSQASHNKMMNPSSHTHPGHAQQTSAVNGRPLPH
TVSTMPHTSGMNRLTQVKTPVQVPLPHPMQMSALGGYSSVSSCNGYGRMG
LLHQEKLPSDLDGMFIERLDCDMESIIRNDLMDGDTLDFNFDNVLPNQSF
PHSVKTTTHSWVSG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3109
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA tag for mammalian expression. Insert contains 3’ UTR.