cDNA Clone FOXO1A

• Plasmid: pEBG-2T-FKHR T24A S256A S319A
• Parent Plasmid: pEBG2T
• Expressed: GST-FKHR T24A S256A S319A
• Genbank: AF032885
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGACGTCCCAGACTACGCTGCCGAGGC
GCCTCAGGTGGTGGAGATCGACCCGGACTTCGAGCCGCTGCCCCGGCCGC
GCTCGTGCGCCTGGCCGCTGCCCAGGCCGGAGTTTAGCCAGTCCAACTCG
GCCACCTCCAGCCCGGCGCCGTCGGGCAGCGCGGCTGCCAACCCCGACGC
CGCGGCGGGCCTGCCCTCGGCCTCGGCTGCCGCTGTCAGCGCCGACTTCA
TGAGCAACCTGAGCTTGCTGGAGGAGAGCGAGGACTTCCCGCAGGCGCCC
GGCTCCGTGGCGGCGGCGGTGGCGGCGGCGGCCGCCGCGGCCGCCACCGG
GGGGCTGTGCGGGGACTTCCAGGGCCCGGAGGCGGGCTGCCTGCACCCAG
CGCCACCGCAGCCCCCGCCGCCCGGGCCGCTGTCGCAGCACCCGCCGGTG
CCCCCCGCCGCCGCTGGGCCGCTCGCGGGGCAGCCGCGCAAGAGCAGCTC
GTCCCGCCGCAACGCGTGGGGCAACCTGTCCTACGCCGACCTCATCACCA
AGGCCATCGAGAGCTCGGCGGAGAAGCGGCTCACGCTGTCGCAGATCTAC
GAGTGGATGGTCAAGAGCGTGCCCTACTTCAAGGATAAGGGTGACAGCAA
CAGCTCGGCGGGCTGGAAGAATTCAATTCGTCATAATCTGTCCCTACACA
GCAAGTTCATTCGTGTGCAGAATGAAGGAACTGGAAAAAGTTCTTGGTGG
ATGCTCAATCCAGAGGGTGGCAAGAGCGGGAAATCTCCTAGGAGAAGAGC
TGCAGCAATGGACAACAACAGTAAATTTGCTAAGAGCCGAAGCCGAGCTG
CCAAGAAGAAAGCATCTCTCCAGTCTGGCCAGGAGGGTGCTGGGGACAGC
CCTGGATCACAGTTTTCCAAATGGCCTGCAAGCCCTGGCTCTCACAGCAA
TGATGACTTTGATAACTGGAGTACATTTCGCCCTCGAACTAGCGCAAATG
CTAGTACTATTAGTGGGAGACTCTCACCCATTATGACCGAACAGGATGAT
CTTGGAGAAGGGGATGTGCATTCTATGGTGTACCCGCCATCTGCCGCAAA
GATGGCCTCTACTTTACCCAGTCTGTCTGAGATAAGCAATCCCGAAAACA
TGGAAAATCTTTTGGATAATCTCAACCTTCTCTCATCACCAACATCATTA
ACTGTTTCGACCCAGTCCTCACCTGGCACCATGATGCAGCAGACGCCGTG
CTACTCGTTTGCGCCACCAAACACCAGTTTGAATTCACCCAGCCCAAACT
ACCAAAAATATACATATGGCCAATCCAGCATGAGCCCTTTGCCCCAGATG
CCTATACAAACACTTCAGGACAATAAGTCGAGTTATGGAGGTATGAGTCA
GTATAACTGTGCGCCTGGACTCTTGAAGGAGTTGCTGACTTCTGACTCTC
CTCCCCATAATGACATTATGACACCAGTTGATCCTGGGGTAGCCCAGCCC
AACAGCCGGGTTCTGGGCCAGAACGTCATGATGGGCCCTAATTCGGTCAT
GTCAACCTATGGCAGCCAGGCATCTCATAACAAAATGATGAATCCCAGCT
CCCATACCCACCCTGGACATGCTCAGCAGACATCTGCAGTTAACGGGCGT
CCCCTGCCCCACACGGTAAGCACCATGCCCCACACCTCGGGTATGAACCG
CCTGACCCAAGTGAAGACACCTGTACAAGTGCCTCTGCCCCACCCCATGC
AGATGAGTGCCCTGGGGGGCTACTCCTCCGTGAGCAGCTGCAATGGCTAT
GGCAGAATGGGCCTTCTCCACCAGGAGAAGCTCCCAAGTGACTTGGATGG
CATGTTCATTGAGCGCTTAGACTGTGACATGGAATCCATCATTCGGAATG
ACCTCATGGATGGAGATACATTGGATTTTAACTTTGACAATGTGTTGCCC
AACCAAAGCTTCCCACACAGTGTCAAGACAACGACACATAGCTGGGTGTC
AGGCTGAGGGTTAGTGAGCAGGTTACACTTAAAAGTACTTCAGATTGTCT
GACAGCAGGAACTGAGAGAAGCAGTCCAAAGATGTCTTTCACCAACTCCC
TTTTAGTTTTCTTGGTTAAAAAAAAAAAAAAAAAAAAGGAATTCGATATC
AAGCTTATCGATACCGTCGACCCGGGCGGCCGCAAAGTTCCCTTTAGTGA
GGGTTAATGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACA
AACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTG
ATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTAAGCCGAATTCTGCAGATATCCATCACACTGGCGGCCGCT
CGAGCATGCATCTAGTGGTACC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSATMYPYDVPDYAAEAPQVVEIDPD
FEPLPRPRSCAWPLPRPEFSQSNSATSSPAPSGSAAANPDAAAGLPSASA
AAVSADFMSNLSLLEESEDFPQAPGSVAAAVAAAAAAAATGGLCGDFQGP
EAGCLHPAPPQPPPPGPLSQHPPVPPAAAGPLAGQPRKSSSSRRNAWGNL
SYADLITKAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSI
RHNLSLHSKFIRVQNEGTGKSSWWMLNPEGGKSGKSPRRRAAAMDNNSKF
AKSRSRAAKKKASLQSGQEGAGDSPGSQFSKWPASPGSHSNDDFDNWSTF
RPRTSANASTISGRLSPIMTEQDDLGEGDVHSMVYPPSAAKMASTLPSLS
EISNPENMENLLDNLNLLSSPTSLTVSTQSSPGTMMQQTPCYSFAPPNTS
LNSPSPNYQKYTYGQSSMSPLPQMPIQTLQDNKSSYGGMSQYNCAPGLLK
ELLTSDSPPHNDIMTPVDPGVAQPNSRVLGQNVMMGPNSVMSTYGSQASH
NKMMNPSSHTHPGHAQQTSAVNGRPLPHTVSTMPHTSGMNRLTQVKTPVQ
VPLPHPMQMSALGGYSSVSSCNGYGRMGLLHQEKLPSDLDGMFIERLDCD
MESIIRNDLMDGDTLDFNFDNVLPNQSFPHSVKTTTHSWVSG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1854
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/HA fusion for mammalian expression. DNA insert contains 3’ UTR region.