cDNA Clone FOXO3A

• Plasmid: pGEX6P-1-FKHR L1 6His
• Parent Plasmid: pGEX6P1
• Expressed: GST-FKHR L1 6His
• Genbank: NP_001446
• Tag: GST

Sequence of Insert

GGATCCGCAGAGGCACCGGCTTCCCCGGCCCCGCTCTCTCCGCTCGAAGT
GGAGCTGGACCCGGAGTTCGAGCCCCAGAGCCGTCCGCGATCCTGTACGT
GGCCCCTGCAAAGGCCGGAGCTCCAAGCGAGCCCTGCCAAGCCCTCGGGG
GAGACGGCCGCTGACTCCATGATCCCCGAGGAGGAGGACGATGAAGACGA
CGAGGACGGCGGGGGACGGGCCGGCTCGGCCATGGCGATCGGCGGCGGCG
GCGGGAGCGGCACGCTGGGCTCCGGGCTGCTCCTTGAGGACTCGGCCCGG
GTGCTGGCACCCGGAGGGCAAGACCCCGGGTCTGGGCCAGCCACCGCGGC
GGGCGGGCTGAGCGGGGGTACACAGGCGCTGCTGCAGCCTCAGCAACCGC
TGCCACCGCCGCAGCCGGGGGCGGCTGGGGGCTCCGGGCAGCCGAGGAAA
TGTTCGTCGCGGCGGAACGCCTGGGGAAACCTGTCCTACGCGGACCTGAT
CACCCGCGCCATCGAGAGCTCCCCGGACAAACGGCTCACTCTGTCCCAGA
TCTACGAGTGGATGGTGCGTTGCGTGCCCTACTTCAAGGATAAGGGCGAC
AGCAACAGCTCTGCCGGCTGGAAGAACTCCATCCGGCACAACCTGTCACT
GCATAGTCGATTCATGCGGGTCCAGAATGAGGGAACTGGCAAGAGCTCTT
GGTGGATCATCAACCCTGATGGGGGGAAGAGCGGAAAAGCCCCCCGGCGG
CGGGCTGTCTCCATGGACAATAGCAACAAGTATACCAAGAGCCGTGGCCG
CGCAGCCAAGAAGAAGGCAGCCCTGCAGACAGCCCCCGAATCAGCTGACG
ACAGTCCCTCCCAGCTCTCCAAGTGGCCTGGCAGCCCCACGTCACGCAGC
AGTGATGAGCTGGATGCGTGGACGGACTTCCGTTCACGCACCAATTCTAA
CGCCAGCACAGTCAGTGGCCGCCTGTCGCCCATCATGGCAAGCACAGAGT
TGGATGAAGTCCAGGACGATGATGCGCCTCTCTCGCCCATGCTCTACAGC
AGCTCAGCCAGCCTGTCACCTTCAGTAAGCAAGCCGTGCACGGTGGAACT
GCCACGGCTGACTGATATGGCAGGCACCATGAATCTGAATGATGGGCTGA
CTGAAAACCTCATGGACGACCTGCTGGATAACATCACGCTCCCGCCATCC
CAGCCATCGCCCACTGGGGGACTCATGCAGCGGAGCTCTAGCTTCCCGTA
TACCACCAAGGGCTCGGGCCTGGGCTCCCCAACCAGCTCCTTTAACAGCA
CGGTGTTCGGACCTTCATCTCTGAACTCCCTACGCCAGTCTCCCATGCAG
ACCATCCAAGAGAACAAGCCAGCTACCTTCTCTTCCATGTCACACTATGG
TAACCAGACACTCCAGGACCTGCTCACTTCGGACTCACTTAGCCACAGCG
ATGTCATGATGACACAGTCGGACCCCTTGATGTCTCAGGCCAGCACCGCT
GTGTCTGCCCAGAATTCCCGCCGGAACGTGATGCTTCGCAATGATCCGAT
GATGTCCTTTGCTGCCCAGCCTAACCAGGGAAGTTTGGTCAATCAGAACT
TGCTCCACCACCAGCACCAAACCCAGGGCGCTCTTGGTGGCAGCCGTGCC
TTGTCGAATTCTGTCAGCAACATGGGCTTGAGTGAGTCCAGCAGCCTTGG
GTCAGCCAAACACCAGCAGCAGTCTCCTGTCAGCCAGTCTATGCAAACCC
TCTCGGACTCTCTCTCAGGCTCCTCCTTGTACTCAACTAGTGCAAACCTG
CCCGTCATGGGCCATGAGAAGTTCCCCAGCGACTTGGACCTGGACATGTT
CAATGGGAGCTTGGAATGTGACATGGAGTCCATTATCCGTAGTGAACTCA
TGGATGCTGATGGGTTGGATTTTAACTTTGATTCCCTCATCTCCACACAG
AATGTTGTTGGTTTGAACGTGGGGAACTTCACTGGTGCTAAGCAGGCCTC
ATCTCAGAGCTGGGTGCCAGGCCATCATCACCATCACCATTAAGTCGACG
C

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAEAPASPAPLSPLEVELDP
EFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGG
GRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLS
GGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAI
ESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRF
MRVQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKK
KAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTV
SGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLT
DMAGTMNLNDGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFPYTTKG
SGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTL
QDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFA
AQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKH
QQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSL
ECDMESIIRSELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSW
VPGHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1716
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST and C terminal 6His for bacterial expression