cDNA Clone FOXO1A

• Plasmid: pGEX-FKHR
• Parent Plasmid: pGEX4T1
• Expressed: GST-FKHR
• Genbank: NM_002015
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGAGTTCATGCCCATGGAGGCCGAGGCGCCTCAGGT
GGTGGAGATCGACCCGGACTTCGAGCCGCTGCCCCGGCCGCGCTCGTGCA
CCTGGCCGCTGCCCAGGCCGGAGTTTAGCCAGTCCAACTCGGCCACCTCC
AGCCCGGCGCCGTCGGGCAGCGCGGCTGCCAACCCCGACGCCGCGGCGGG
CCTGCCCTCGGCCTCGGCTGCCGCTGTCAGCGCCGACTTCATGAGCAACC
TGAGCTTGCTGGAGGAGAGCGAGGACTTCCCGCAGGCGCCCGGCTCCGTG
GCGGCGGCGGTGGCGGCGGCGGCCGCCGCGGCCGCCACCGGGGGGCTGTG
CGGGGACTTCCAGGGCCCGGAGGCGGGCTGCCTGCACCCAGCGCCACCGC
AGCCCCCGCCGCCCGGGCCGCTGTCGCAGCACCCGCCGGTGCCCCCCGCC
GCCGCTGGGCCGCTCGCGGGGCAGCCGCGCAAGAGCAGCTCGTCCCGCCG
CAACGCGTGGGGCAACCTGTCCTACGCCGACCTCATCACCAAGGCCATCG
AGAGCTCGGCGGAGAAGCGGCTCACGCTGTCGCAGATCTACGAGTGGATG
GTCAAGAGCGTGCCCTACTTCAAGGATAAGGGTGACAGCAACAGCTCGGC
GGGCTGGAAGAATTCAATTCGTCATAATCTGTCCCTACACAGCAAGTTCA
TTCGTGTGCAGAATGAAGGAACTGGAAAAAGTTCTTGGTGGATGCTCAAT
CCAGAGGGTGGCAAGAGCGGGAAATCTCCTAGGAGAAGAGCTGCATCCAT
GGACAACAACAGTAAATTTGCTAAGAGCCGAAGCCGAGCTGCCAAGAAGA
AAGCATCTCTCCAGTCTGGCCAGGAGGGTGCTGGGGACAGCCCTGGATCA
CAGTTTTCCAAATGGCCTGCAAGCCCTGGCTCTCACAGCAATGATGACTT
TGATAACTGGAGTACATTTCGCCCTCGAACTAGCTCAAATGCTAGTACTA
TTAGTGGGAGACTCTCACCCATTATGACCGAACAGGATGATCTTGGAGAA
GGGGATGTGCATTCTATGGTGTACCCGCCATCTGCCGCAAAGATGGCCTC
TACTTTACCCAGTCTGTCTGAGATAAGCAATCCCGAAAACATGGAAAATC
TTTTGGATAATCTCAACCTTCTCTCATCACCAACATCATTAACTGTTTCG
ACCCAGTCCTCACCTGGCACCATGATGCAGCAGACGCCGTGCTACTCGTT
TGCGCCACCAAACACCAGTTTGAATTCACCCAGCCCAAACTACCAAAAAT
ATACATATGGCCAATCCAGCATGAGCCCTTTGCCCCAGATGCCTATACAA
ACACTTCAGGACAATAAGTCGAGTTATGGAGGTATGAGTCAGTATAACTG
TGCGCCTGGACTCTTGAAGGAGTTGCTGACTTCTGACTCTCCTCCCCATA
ATGACATTATGACACCAGTTGATCCTGGGGTAGCCCAGCCCAACAGCCGG
GTTCTGGGCCAGAACGTCATGATGGGCCCTAATTCGGTCATGTCAACCTA
TGGCAGCCAGGCATCTCATAACAAAATGATGAATCCCAGCTCCCATACCC
ACCCTGGACATGCTCAGCAGACATCTGCAGTTAACGGGCGTCCCCTGCCC
CACACGGTAAGCACCATGCCCCACACCTCGGGTATGAACCGCCTGACCCA
AGTGAAGACACCTGTACAAGTGCCTCTGCCCCACCCCATGCAGATGAGTG
CCCTGGGGGGCTACTCCTCCGTGAGCAGCTGCAATGGCTATGGCAGAATG
GGCCTTCTCCACCAGGAGAAGCTCCCAAGTGACTTGGATGGCATGTTCAT
TGAGCGCTTAGACTGTGACATGGAATCCATCATTCGGAATGACCTCATGG
ATGGAGATACATTGGATTTTAACTTTGACAATGTGTTGCCCAACCAAAGC
TTCCCACACAGTGTCAAGACAACGACACATAGCTGGGTGTCAGGCTGAGG
GTTAAGCCGAATTCTGCAGATATCCATCACAGTGGCGGCCGCTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSATMEFMPMEAEAPQVVEIDPDFEP
LPRPRSCTWPLPRPEFSQSNSATSSPAPSGSAAANPDAAAGLPSASAAAV
SADFMSNLSLLEESEDFPQAPGSVAAAVAAAAAAAATGGLCGDFQGPEAG
CLHPAPPQPPPPGPLSQHPPVPPAAAGPLAGQPRKSSSSRRNAWGNLSYA
DLITKAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSIRHN
LSLHSKFIRVQNEGTGKSSWWMLNPEGGKSGKSPRRRAASMDNNSKFAKS
RSRAAKKKASLQSGQEGAGDSPGSQFSKWPASPGSHSNDDFDNWSTFRPR
TSSNASTISGRLSPIMTEQDDLGEGDVHSMVYPPSAAKMASTLPSLSEIS
NPENMENLLDNLNLLSSPTSLTVSTQSSPGTMMQQTPCYSFAPPNTSLNS
PSPNYQKYTYGQSSMSPLPQMPIQTLQDNKSSYGGMSQYNCAPGLLKELL
TSDSPPHNDIMTPVDPGVAQPNSRVLGQNVMMGPNSVMSTYGSQASHNKM
MNPSSHTHPGHAQQTSAVNGRPLPHTVSTMPHTSGMNRLTQVKTPVQVPL
PHPMQMSALGGYSSVSSCNGYGRMGLLHQEKLPSDLDGMFIERLDCDMES
IIRNDLMDGDTLDFNFDNVLPNQSFPHSVKTTTHSWVSG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1711
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/ EE tag for bacterial expression.