cDNA Clone FOXO1A

• Plasmid: pEBG-2T-FKHR wt
• Parent Plasmid: pEBG2T
• Expressed: FKHR GST wt
• Genbank: NM_002015
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGACGTCCCAGACTACGCTGCCGAGGC
GCCTCAGGTGGTGGAGATCGACCCGGACTTCGAGCCGCTGCCCCGGCCGC
GCTCGTGCACCTGGCCGCTGCCCAGGCCGGAGTTTAGCCAGTCCAACTCG
GCCACCTCCAGCCCGGCGCCGTCGGGCAGCGCGGCTGCCAACCCCGACGC
CGCGGCGGGCCTGCCCTCGGCCTCGGCTGCCGCTGTCAGCGCCGACTTCA
TGAGCAACCTGAGCTTGCTGGAGGAGAGCGAGGACTTCCCGCAGGCGCCC
GGCTCCGTGGCGGCGGCGGTGGCGGCGGCGGCCGCCGCGGCCGCCACCGG
GGGGCTGTGCGGGGACTTCCAGGGCCCGGAGGCGGGCTGCCTGCACCCAG
CGCCACCGCAGCCCCCGCCGCCCGGGCCGCTGTCGCAGCACCCGCCGGTG
CCCCCCGCCGCCGCTGGGCCGCTCGCGGGGCAGCCGCGCAAGAGCAGCTC
GTCCCGCCGCAACGCGTGGGGCAACCTGTCCTACGCCGACCTCATCACCA
AGGCCATCGAGAGCTCGGCGGAGAAGCGGCTCACGCTGTCGCAGATCTAC
GAGTGGATGGTCAAGAGCGTGCCCTACTTCAAGGATAAGGGTGACAGCAA
CAGCTCGGCGGGCTGGAAGAATTCAATTCGTCATAATCTGTCCCTACACA
GCAAGTTCATTCGTGTGCAGAATGAAGGAACTGGAAAAAGTTCTTGGTGG
ATGCTCAATCCAGAGGGTGGCAAGAGCGGGAAATCTCCTAGGAGAAGAGC
TGCATCCATGGACAACAACAGTAAATTTGCTAAGAGCCGAAGCCGAGCTG
CCAAGAAGAAAGCATCTCTCCAGTCTGGCCAGGAGGGTGCTGGGGACAGC
CCTGGATCACAGTTTTCCAAATGGCCTGCAAGCCCTGGCTCTCACAGCAA
TGATGACTTTGATAACTGGAGTACATTTCGCCCTCGAACTAGCTCAAATG
CTAGTACTATTAGTGGGAGACTCTCACCCATTATGACCGAACAGGATGAT
CTTGGAGAAGGGGATGTGCATTCTATGGTGTACCCGCCATCTGCCGCAAA
GATGGCCTCTACTTTACCCAGTCTGTCTGAGATAAGCAATCCCGAAAACA
TGGAAAATCTTTTGGATAATCTCAACCTTCTCTCATCACCAACATCATTA
ACTGTTTCGACCCAGTCCTCACCTGGCACCATGATGCAGCAGACGCCGTG
CTACTCGTTTGCGCCACCAAACACCAGTTTGAATTCACCCAGCCCAAACT
ACCAAAAATATACATATGGCCAATCCAGCATGAGCCCTTTGCCCCAGATG
CCTATACAAACACTTCAGGACAATAAGTCGAGTTATGGAGGTATGAGTCA
GTATAACTGTGCGCCTGGACTCTTGAAGGAGTTGCTGACTTCTGACTCTC
CTCCCCATAATGACATTATGACACCAGTTGATCCTGGGGTAGCCCAGCCC
AACAGCCGGGTTCTGGGCCAGAACGTCATGATGGGCCCTAATTCGGTCAT
GTCAACCTATGGCAGCCAGGCATCTCATAACAAAATGATGAATCCCAGCT
CCCATACCCACCCTGGACATGCTCAGCAGACATCTGCAGTTAACGGGCGT
CCCCTGCCCCACACGGTAAGCACCATGCCCCACACCTCGGGTATGAACCG
CCTGACCCAAGTGAAGACACCTGTACAAGTGCCTCTGCCCCACCCCATGC
AGATGAGTGCCCTGGGGGGCTACTCCTCCGTGAGCAGCTGCAATGGCTAT
GGCAGAATGGGCCTTCTCCACCAGGAGAAGCTCCCAAGTGACTTGGATGG
CATGTTCATTGAGCGCTTAGACTGTGACATGGAATCCATCATTCGGAATG
ACCTCATGGATGGAGATACATTGGATTTTAACTTTGACAATGTGTTGCCC
AACCAAAGCTTCCCACACAGTGTCAAGACAACGACACATAGCTGGGTGTC
AGGCTGAGGGTTAGTGAGCAGGTTACACTTAAAAGTACTTCAGATTGTCT
GACAGCAGGAACTGAGAGAAGCAGTCCAAAGATGTCTTTCACCAACTCCC
TTTTAGTTTTCTCGGTTAAAAAAAAAAAAAAAAAAAAGGAATTCGATATC
AAGCTTATCGATACCGTCGACCCGGGCGGCCGCAAAGTTCCCTTTAGTGA
GGGTTAATGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACA
AACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTG
ATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTAAGCCGAATTCTGCAGATATCCATCACACTGGCGGCCGCT
CGAGCATGCATCTAGTGGTACC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSATMYPYDVPDYAAEAPQVVEIDPD
FEPLPRPRSCTWPLPRPEFSQSNSATSSPAPSGSAAANPDAAAGLPSASA
AAVSADFMSNLSLLEESEDFPQAPGSVAAAVAAAAAAAATGGLCGDFQGP
EAGCLHPAPPQPPPPGPLSQHPPVPPAAAGPLAGQPRKSSSSRRNAWGNL
SYADLITKAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSI
RHNLSLHSKFIRVQNEGTGKSSWWMLNPEGGKSGKSPRRRAASMDNNSKF
AKSRSRAAKKKASLQSGQEGAGDSPGSQFSKWPASPGSHSNDDFDNWSTF
RPRTSSNASTISGRLSPIMTEQDDLGEGDVHSMVYPPSAAKMASTLPSLS
EISNPENMENLLDNLNLLSSPTSLTVSTQSSPGTMMQQTPCYSFAPPNTS
LNSPSPNYQKYTYGQSSMSPLPQMPIQTLQDNKSSYGGMSQYNCAPGLLK
ELLTSDSPPHNDIMTPVDPGVAQPNSRVLGQNVMMGPNSVMSTYGSQASH
NKMMNPSSHTHPGHAQQTSAVNGRPLPHTVSTMPHTSGMNRLTQVKTPVQ
VPLPHPMQMSALGGYSSVSSCNGYGRMGLLHQEKLPSDLDGMFIERLDCD
MESIIRNDLMDGDTLDFNFDNVLPNQSFPHSVKTTTHSWVSG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU360
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/HA tag for mammalian expression. Insert contains 3’ UTR