cDNA Clone Flagellin

• Plasmid: pET28 GFP P FLAGellin
• Parent Plasmid: pET28a original
• Expressed: GST FLAGellin
• Genbank: AAU27422.1
• Tag: GFP
• Species: Legionella pneumophila

Sequence of Insert

CCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTG
GTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGA
GGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCA
CCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACC
TACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGA
CTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCT
TCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAG
GGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGA
GGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACA
ACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTC
ACGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTA
CCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACC
ACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGC
GATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGG
CATGGACGAGCTGTACAAGTCCGGACTCCTGGAAGTTCTGTTCCAGGGGC
CCGGGGGATCCGAATTCAGAGCTCCGTCGACAAGCTTGCGGCCGCACTCG
AGCACC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFTIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLLEVLFQGP
GGSMAQVINTNVASLTAQRNLGVSGNMMQTSIQRLSSGLRINSAKDDAAG
LAISQRMTAQIRGMNQAVRNANDGISLAQVAEGAMQETTNILQRMRELSV
QAANSTNNSSDRSSIQSEISQLKSELERIAQNTEFNGQRILDGSFSGASF
QVGANSNQTINFSIGSTKASSLGGIATATGTEVAGAAAADITIAIGGGAA
TSINSSANFTGALNGQDATSAYAKAAAINDAGIGGLSVTASTSGTQAVGA
IGGTAGDTYNLTINGVAIYTNLNVATALTNSDLRDAINGVSNQTGVVASL
NGGNMTLTAADGRNITVTESGTGFTAGTDGLTVTGGAFDGALRGTLSISA
VDTIAIGGTVANIGLSANISKDTVGIDSLDVSTASGAQTAIKRIDAALNS
VNSNRANMGALQNRFESTIANLQNVSDNLSAARSRIQDADYAAEMASLTK
NQILQQAGTAMLAQANSLPQSVLSLLGR*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU32907
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Legionella pneumophila subsp. pneumophila str. Philadelphia 1; Bacterial N ter GFP vector; ; Estimated MW of expressed protein : 76078 Daltons