cDNA Clone Flagellin

• Plasmid: pGEX6P-1-FLAG FLAGellin
• Parent Plasmid: pGEX6P1
• Genbank: AAU27422.1
• Tag: FLAG
• Species: Legionella pneumophila

Sequence of Insert

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGATGGCTCAAGT
AATCAACACTAATGTGGCGTCGCTCACAGCCCAACGTAATTTGGGTGTTT
CGGGCAACATGATGCAAACATCGATCCAGCGTTTATCATCGGGATTAAGG
ATTAACAGTGCCAAAGATGATGCAGCAGGACTCGCCATTTCTCAACGAAT
GACTGCACAAATTCGCGGGATGAACCAAGCCGTTAGAAATGCCAACGATG
GTATTTCCCTGGCTCAGGTTGCTGAAGGAGCAATGCAGGAAACAACCAAT
ATTTTACAACGCATGCGTGAGCTTTCCGTTCAAGCTGCTAACTCAACCAA
TAACTCATCTGACCGGTCTTCAATTCAAAGTGAAATTTCACAATTAAAAA
GTGAATTGGAGCGTATTGCTCAAAATACTGAATTCAATGGTCAAAGAATT
CTCGATGGTTCTTTCTCTGGCGCAAGCTTCCAGGTTGGGGCGAATTCAAA
CCAAACCATCAATTTCAGTATCGGCAGCACAAAAGCTTCTTCTCTTGGTG
GTATTGCCACGGCAACAGGAACAGAAGTAGCAGGTGCAGCAGCGGCAGAT
ATTACTATCGCAATTGGAGGAGGAGCAGCAACCAGTATTAACTCTTCTGC
CAATTTTACCGGGGCACTAAACGGACAAGATGCTACGTCAGCCTATGCCA
AAGCAGCGGCTATCAACGATGCCGGGATAGGAGGACTATCGGTTACAGCG
TCTACCAGCGGCACACAAGCAGTTGGCGCAATAGGTGGAACTGCAGGTGA
TACCTACAACTTAACGATTAATGGTGTTGCAATATACACTAACCTTAACG
TAGCAACGGCATTAACCAACTCTGATCTTAGAGATGCCATCAATGGCGTC
AGTAACCAAACTGGAGTTGTTGCATCACTTAACGGTGGTAATATGACACT
CACTGCTGCTGATGGCAGAAACATTACGGTAACAGAAAGCGGTACAGGTT
TTACTGCAGGTACTGACGGTTTAACTGTAACTGGTGGCGCATTTGATGGC
GCGCTACGCGGTACTTTATCAATCAGCGCCGTTGATACTATCGCGATTGG
TGGTACTGTTGCTAACATTGGTTTAAGTGCCAATATCTCTAAAGACACAG
TAGGGATAGATTCACTGGATGTGAGCACAGCGTCTGGCGCTCAAACAGCG
ATTAAGAGAATTGATGCAGCACTCAATAGCGTAAACTCTAATCGGGCTAA
TATGGGTGCTCTGCAAAATCGCTTTGAATCAACGATAGCCAACCTGCAAA
ACGTATCAGACAACTTATCAGCTGCTAGAAGTCGTATTCAAGATGCTGAT
TACGCTGCTGAAATGGCTAGTTTGACCAAAAACCAAATTCTGCAACAAGC
AGGTACAGCGATGTTGGCACAAGCTAATAGCCTACCGCAATCTGTATTAT
CTTTGTTAGGTCGATAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATMDYKDDDDKMAQVINTN
VASLTAQRNLGVSGNMMQTSIQRLSSGLRINSAKDDAAGLAISQRMTAQI
RGMNQAVRNANDGISLAQVAEGAMQETTNILQRMRELSVQAANSTNNSSD
RSSIQSEISQLKSELERIAQNTEFNGQRILDGSFSGASFQVGANSNQTIN
FSIGSTKASSLGGIATATGTEVAGAAAADITIAIGGGAATSINSSANFTG
ALNGQDATSAYAKAAAINDAGIGGLSVTASTSGTQAVGAIGGTAGDTYNL
TINGVAIYTNLNVATALTNSDLRDAINGVSNQTGVVASLNGGNMTLTAAD
GRNITVTESGTGFTAGTDGLTVTGGAFDGALRGTLSISAVDTIAIGGTVA
NIGLSANISKDTVGIDSLDVSTASGAQTAIKRIDAALNSVNSNRANMGAL
QNRFESTIANLQNVSDNLSAARSRIQDADYAAEMASLTKNQILQQAGTAM
LAQANSLPQSVLSLLGR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12874
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Species : Legionella pneumophila subsp. pneumophila str. Philadelphia 1.; N ter FLAG/GST for bacterial expression.; ; Estimated MW of expressed protein : 75962 Daltons