cDNA Clone FLJ21128

• Plasmid: pEBG6P-FLJ21128
• Parent Plasmid: pEBG6P
• Expressed: GST-FLJ21128
• Genbank: NP_079359
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCTACAGATTGGCTGGGAAGTATTGTGTCCATCAATTGTGG
AGATAGCTTGGGTGTCTATCAGGGAAGAGTGTCAGCTGTGGATCAGGTCA
GCCAGACCATTTCTCTCACCCGGCCTTTCCATAATGGAGTGAAGTGTCTT
GTTCCAGAAGTCACCTTCAGGGCAGGTGACATTACGGAGTTAAAAATTCT
GGAGATACCAGGACCTGGAGACAACCAACATTTTGGAGACCTTCATCAAA
CAGAATTAGGCCCCTCTGGTGCTGGCTGCCAAGTGGGCATCAATCAGAAT
GGCACAGGCAAGTTTGTCAAGAAGCCAGCCTCTTCCAGCAGTGCCCCTCA
GAATATCCCTAAGAGGACAGATGTGAAGAGCCAGGATGTTGCCGTTTCCC
CGCAGCAGCAACAGTGCTCAAAGAGCTATGTCGACAGGCACATGGAATCC
TTGAGTCAGTCCAAAAGTTTCCGTCGTCGGCACAACTCCTGGTCATCTAG
TAGCAGGCACCCAAATCAGGCAACTCCCAAGAAAAGTGGTTTAAAGAATG
GCCAGATGAAGAATAAAGATGACGAGTGCTTCGGGGATGATATTGAGGAG
ATCCCAGACACAGATTTTGATTTTGAAGGGAACCTGGCTCTTTTTGACAA
GGCAGCTGTGTTTGAGGAGATTGATACCTATGAAAGGAGAAGTGGTACCC
GTTCCCGGGGCATCCCAAATGAAAGGCCCACTCGGTACCGCCATGATGAG
AACATCTTGGAGTCCGAGCCCATTGTCTATCGACGGATCATAGTGCCCCA
CAACGTGAGCAAGGAGTTCTGCACGGACTCTGGCCTGGTTGTCCCAAGTA
TTTCCTATGAGCTGCATAAAAAGCTGTTGTCCGTGGCTGAGAAGCATGGG
CTGACCCTTGAGCGGAGACTGGAGATGACAGGTGTGTGTGCCAGTCAGAT
GGCACTGACCCTCCTCGGAGGACCTAACAGGTTGAATCCCAAAAATGTTC
ACCAGAGGCCTACAGTGGCTCTACTGTGTGGACCTCATGTGAAGGGGGCT
CAGGGTATCAGCTGTGGAAGGCACCTAGCCAACCATGATGTCCAGGTCAT
CCTTTTCCTGCCCAATTTTGTCAAGATGTTGGAATCTATCACCAATGAGC
TGTCGCTCTTCAGCAAGACCCAAGGCCAACAAGTGTCTAGCCTCAAAGAT
CTGCCCACTAGCCCTGTGGACCTGGTCATCAACTGCCTGGATTGCCCTGA
GAACGTCTTCCTGCGCGATCAACCCTGGTACAAGGCAGCTGTGGCCTGGG
CCAACCAGAACCGGGCACCAGTACTCAGCATAGACCCTCCTGTGCATGAA
GTCGAACAGGGCATTGATGCCAAATGGTCACTGGCACTGGGCCTGCCTCT
GCCACTGGGGGAGCACGCAGGCCGTATCTATTTGTGCGACATTGGCATTC
CCCAGCAGGTCTTCCAGGAGGTGGGCATCAACTACCACTCGCCCTTTGGC
TGCAAGTTTGTTATCCCACTGCACTCTGCTTAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMATDWLGSIVSIN
CGDSLGVYQGRVSAVDQVSQTISLTRPFHNGVKCLVPEVTFRAGDITELK
ILEIPGPGDNQHFGDLHQTELGPSGAGCQVGINQNGTGKFVKKPASSSSA
PQNIPKRTDVKSQDVAVSPQQQQCSKSYVDRHMESLSQSKSFRRRHNSWS
SSSRHPNQATPKKSGLKNGQMKNKDDECFGDDIEEIPDTDFDFEGNLALF
DKAAVFEEIDTYERRSGTRSRGIPNERPTRYRHDENILESEPIVYRRIIV
PHNVSKEFCTDSGLVVPSISYELHKKLLSVAEKHGLTLERRLEMTGVCAS
QMALTLLGGPNRLNPKNVHQRPTVALLCGPHVKGAQGISCGRHLANHDVQ
VILFLPNFVKMLESITNELSLFSKTQGQQVSSLKDLPTSPVDLVINCLDC
PENVFLRDQPWYKAAVAWANQNRAPVLSIDPPVHEVEQGIDAKWSLALGL
PLPLGEHAGRIYLCDIGIPQQVFQEVGINYHSPFGCKFVIPLHSA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8034
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.; Estimated MW of expressed protein : 83442 Daltons