cDNA Clone FLJ21128

• Plasmid: pGEX6P-1-FLJ21128
• Parent Plasmid: pGEX6P1
• Expressed: GST-FLJ21128
• Genbank: NP_079359
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCTACAGATTGGCTGGGAAGTATTGTGTCCATCAATTGTGG
AGATAGCTTGGGTGTCTATCAGGGAAGAGTGTCAGCTGTGGATCAGGTCA
GCCAGACCATTTCTCTCACCCGGCCTTTCCATAATGGAGTGAAGTGTCTT
GTTCCAGAAGTCACCTTCAGGGCAGGTGACATTACGGAGTTAAAAATTCT
GGAGATACCAGGACCTGGAGACAACCAACATTTTGGAGACCTTCATCAAA
CAGAATTAGGCCCCTCTGGTGCTGGCTGCCAAGTGGGCATCAATCAGAAT
GGCACAGGCAAGTTTGTCAAGAAGCCAGCCTCTTCCAGCAGTGCCCCTCA
GAATATCCCTAAGAGGACAGATGTGAAGAGCCAGGATGTTGCCGTTTCCC
CGCAGCAGCAACAGTGCTCAAAGAGCTATGTCGACAGGCACATGGAATCC
TTGAGTCAGTCCAAAAGTTTCCGTCGTCGGCACAACTCCTGGTCATCTAG
TAGCAGGCACCCAAATCAGGCAACTCCCAAGAAAAGTGGTTTAAAGAATG
GCCAGATGAAGAATAAAGATGACGAGTGCTTCGGGGATGATATTGAGGAG
ATCCCAGACACAGATTTTGATTTTGAAGGGAACCTGGCTCTTTTTGACAA
GGCAGCTGTGTTTGAGGAGATTGATACCTATGAAAGGAGAAGTGGTACCC
GTTCCCGGGGCATCCCAAATGAAAGGCCCACTCGGTACCGCCATGATGAG
AACATCTTGGAGTCCGAGCCCATTGTCTATCGACGGATCATAGTGCCCCA
CAACGTGAGCAAGGAGTTCTGCACGGACTCTGGCCTGGTTGTCCCAAGTA
TTTCCTATGAGCTGCATAAAAAGCTGTTGTCCGTGGCTGAGAAGCATGGG
CTGACCCTTGAGCGGAGACTGGAGATGACAGGTGTGTGTGCCAGTCAGAT
GGCACTGACCCTCCTCGGAGGACCTAACAGGTTGAATCCCAAAAATGTTC
ACCAGAGGCCTACAGTGGCTCTACTGTGTGGACCTCATGTGAAGGGGGCT
CAGGGTATCAGCTGTGGAAGGCACCTAGCCAACCATGATGTCCAGGTCAT
CCTTTTCCTGCCCAATTTTGTCAAGATGTTGGAATCTATCACCAATGAGC
TGTCGCTCTTCAGCAAGACCCAAGGCCAACAAGTGTCTAGCCTCAAAGAT
CTGCCCACTAGCCCTGTGGACCTGGTCATCAACTGCCTGGATTGCCCTGA
GAACGTCTTCCTGCGCGATCAACCCTGGTACAAGGCAGCTGTGGCCTGGG
CCAACCAGAACCGGGCACCAGTACTCAGCATAGACCCTCCTGTGCATGAA
GTCGAACAGGGCATTGATGCCAAATGGTCACTGGCACTGGGCCTGCCTCT
GCCACTGGGGGAGCACGCAGGCCGTATCTATTTGTGCGACATTGGCATTC
CCCAGCAGGTCTTCCAGGAGGTGGGCATCAACTACCACTCGCCCTTTGGC
TGCAAGTTTGTTATCCCACTGCACTCTGCTTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMATDWLGSIVSINCGDSLG
VYQGRVSAVDQVSQTISLTRPFHNGVKCLVPEVTFRAGDITELKILEIPG
PGDNQHFGDLHQTELGPSGAGCQVGINQNGTGKFVKKPASSSSAPQNIPK
RTDVKSQDVAVSPQQQQCSKSYVDRHMESLSQSKSFRRRHNSWSSSSRHP
NQATPKKSGLKNGQMKNKDDECFGDDIEEIPDTDFDFEGNLALFDKAAVF
EEIDTYERRSGTRSRGIPNERPTRYRHDENILESEPIVYRRIIVPHNVSK
EFCTDSGLVVPSISYELHKKLLSVAEKHGLTLERRLEMTGVCASQMALTL
LGGPNRLNPKNVHQRPTVALLCGPHVKGAQGISCGRHLANHDVQVILFLP
NFVKMLESITNELSLFSKTQGQQVSSLKDLPTSPVDLVINCLDCPENVFL
RDQPWYKAAVAWANQNRAPVLSIDPPVHEVEQGIDAKWSLALGLPLPLGE
HAGRIYLCDIGIPQQVFQEVGINYHSPFGCKFVIPLHSA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8032
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; Estimated MW of expressed protein : 82828 Daltons