cDNA Clone FKHRL1

• Plasmid: pGEX6P-1-FKHRL1
• Parent Plasmid: pGEX6P1
• Expressed: GST-FKHRL1
• Genbank: NM_001455
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCAGAGGCACCGGCTTCCCCGGCCCCGCTCTCTCCGCTCGAAGT
GGAGCTGGACCCGGAGTTCGAGCCCCAGAGCCGTCCGCGATCCTGTACGT
GGCCCCTGCAAAGGCCGGAGCTCCAAGCGAGCCCTGCCAAGCCCTCGGGG
GAGACGGCCGCCGACTCCATGATCCCCGAGGAGGAGGACGATGAAGACGA
CGAGGACGGCGGGGGACGGGCCGGCTCGGCCATGGCGATCGGCGGCGGCG
GCGGGAGCGGCACGCTGGGCTCCGGGCTGCTCCTTGAGGACTCGGCCCGG
GTGCTGGCACCCGGAGGGCAAGACCCCGGGTCTGGGCCAGCCACCGCGGC
GGGCGGGCTGAGCGGGGGTACACAGGCGCTGCTGCAGCCTCAGCAACCGC
TGCCACCGCCGCAGCCGGGGGCGGCTGGGGGCTCCGGGCAGCCGAGGAAA
TGTTCGTCGCGGCGGAACGCCTGGGGAAACCTGTCCTACGCGGACCTGAT
CACCCGCGCCATCGAGAGCTCCCCGGACAAACGGCTCACTCTGTCCCAGA
TCTACGAGTGGATGGTGCGTTGCGTGCCCTACTTCAAGGATAAGGGCGAC
AGCAACAGCTCTGCCGGCTGGAAGAACTCCATCCGGCACAACCTGTCACT
GCATAGTCGATTCATGCGGGTCCAGAATGAGGGAACTGGCAAGAGCTCTT
GGTGGATCATCAACCCTGATGGGGGGAAGAGCGGAAAAGCCCCCCGGCGG
CGGGCTGTCTCCATGGACAATAGCAACAAGTATACCAAGAGCCGTGGCCG
CGCAGCCAAGAAGAAGGCAGCCCTGCAGACAGCCCCCGAATCAGCTGACG
ACAGTCCCTCCCAGCTCTCCAAGTGGCCTGGCAGCCCCACGTCACGCAGC
AGTGATGAGCTGGATGCGTGGACGGACTTCCGTTCACGCACCAATTCTAA
CGCCAGCACAGTCAGTGGCCGCCTGTCGCCCATCATGGCAAGCACAGAGT
TGGATGAAGTCCAGGACGATGATGCGCCTCTCTCGCCCATGCTCTACAGC
AGCTCAGCCAGCCTGTCACCTTCAGTAAGCAAGCCGTGCACGGTGGAACT
GCCACGGCTGACTGATATGGCAGGCACCATGAATCTGAATGATGGGCTGA
CTGAAAACCTCATGGACGACCTGCTGGATAACATCACGCTCCCGCCATCC
CAGCCATCGCCCACTGGGGGACTCATGCAGCGGAGCTCTAGCTTCCCGTA
TACCACCAAGGGCTCGGGCCTGGGCTCCCCAACCAGCTCCTTTAACAGCA
CGGTGTTCGGACCTTCATCTCTGAACTCCCTACGCCAGTCTCCCATGCAG
ACCATCCAAGAGAACAAGCCAGCTACCTTCTCTTCCATGTCACACTATGG
TAACCAGACACTCCAGGACCTGCTCACTTCGGACTCACTTAGCCACAGCG
ATGTCATGATGACACAGTCGGACCCCTTGATGTCTCAGGCCAGCACCGCT
GTGTCTGCCCAGAATTCCCGCCGGAACGTGATGCTTCGCAATGATCCGAT
GATGTCCTTTGCTGCCCAGCCTAACCAGGGAAGTTTGGTCAATCAGAACT
TGCTCCACCACCAGCACCAAACCCAGGGCGCTCTTGGTGGCAGCCGTGCC
TTGTCGAATTCTGTCAGCAACATGGGCTTGAGTGAGTCCAGCAGCCTTGG
GTCAGCCAAACACCAGCAGCAGTCTCCTGTCAGCCAGTCTATGCAAACCC
TCTCGGACTCTCTCTCAGGCTCCTCCTTGTACTCAACTAGTGCAAACCTG
CCCGTCATGGGCCATGAGAAGTTCCCCAGCGACTTGGACCTGGACATGTT
CAATGGGAGCTTGGAATGTGACATGGAGTCCATTATCCGTAGTGAACTCA
TGGATGCTGATGGGTTGGATTTTAACTTTGATTCCCTCATCTCCACACAG
AATGTTGTTGGTTTGAACGTGGGGAACTTCACTGGTGCTAAGCAGGCCTC
ATCTCAGAGCTGGGTGCCAGGCTGAGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1069
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion