cDNA Clone DISC1

• Plasmid: pMalP2X DISC1
• Parent Plasmid: pMALP2X
• Expressed: MBP-DISC1
• Genbank: AF222980.1 GI:8163868
• Tag: MBP
• Species: Human

Sequence of Insert

GAATTCATGCCAGGCGGGGGTCCTCAGGGCGCCCCAGCCGCCGCCGGCGGCGGCGGCGTGAGCCACCGCGCAGGCAGCCGGGATTGCTTACCACCTGCAGCGTGCTTTCGGAGGCGGCGGCTGGCACGGAGGCCGGGCTACATGAGAAGCTCGACAGGGCCTGGGATCGGGTTCCTTTCCCCAGCAGTGGGCACACTGTTCCGGTTCCCAGGAGGGGTGTCTGGCGAGGAGTCCCACCACTCGGAGTCCAGGGCCAGACAGTGTGGCCTTGACTCGAGAGGCCTCTTGGTCCGGAGCCCTGTTTCC
AAGAGTGCAGCAGCCCCTACTGTGACCTCTGTGAGAGGAACCTCGGCGCA
CTTTGGGATTCAGCTCAGAGGTGGCACCAGATTGCCTGACAGGCTTAGCT
GGCCGTGTGGCCCTGGGAGTGCTGGGTGGCAGCAAGAGTTTGCAGCCATG
GATAGTTCTGAGACCCTGGACGCCAGCTGGGAGGCAGCCTGCAGCGATGG
AGCAAGGCGTGTCCGGGCAGCAGGCTCTCTGCCATCAGCAGAGTTGAGTA
GCAACAGCTGCAGCCCTGGCTGTGGCCCTGAGGTCCCCCCAACCCCTCCT
GGCTCTCACAGTGCCTTTACCTCAAGCTTTAGCTTTATTCGGCTCTCGCT
TGGCTCTGCCGGGGAACGTGGAGAAGCAGAAGGCTGCCCACCATCCAGAG
AGGCTGAGTCCCATTGCCAGAGCCCCCAGGAGATGGGAGCCAAAGCTGCC
AGCTTGGACGGGCCTCACGAGGACCCGCGATGTCTCTCTCAGCCCTTCAG
TCTCTTGGCTACACGGGTCTCTGCAGACTTGGCCCAGGCCGCAAGGAACA
GCTCCAGGCCAGAGCGTGACATGCATTCTTTACCAGACATGGACCCTGGC
TCCTCCAGTTCTCTGGATCCCTCACTGGCTGGCTGTGGTGGTGATGGGAG
CAGCGGCTCAGGGGATGCCCACTCTTGGGACACCCTGCTCAGGAAATGGG
AGCCAGTGCTGCGGGACTGCCTGCTGAGAAACCGGAGGCAGATGGAGGTA
ATATCCTTAAGATTAAAACTTCAGAAACTTCAGGAAGATGCAGTTGAGAA
TGATGATTATGATAAAGCTGAGACGTTACAACAAAGATTAGAAGACCTGG
AACAAGAGAAAATCAGCCTGCACTTTCAACTTCCTTCAAGGCAGCCAGCT
CTTAGCAGTTTCCTGGGTCACCTGGCAGCACAAGTCCAGGCTGCCTTGCG
CCGTGGGGCCACTCAGCAGGCCAGCGGAGATGACACCCACACCCCACTGA
GAATGGAGCCGAGGCTGTTGGAACCCACTGCTCAGGACAGCTTGCACGTG
TCCATCACGAGACGAGACTGGCTTCTTCAGGAAAAGCAGCAGCTACAGAA
AGAAATCGAAGCTCTCCAAGCAAGGATGTTTGTGCTGGAAGCCAAAGATC
AACAGCTGAGAAGGGAAATAGAGGAGCAAGAGCAGCAACTCCAGTGGCAG
GGCTGCGACCTGACCCCACTGGTGGGCCAGCTGTCCCTGGGTCAGCTGCA
GGAGGTCAGCAAGGCCTTGCAGGACACCCTGGCCTCAGCCGGTCAGATTC
CCTTCCATGCAGAGCCACCGGAAACCATAAGGAGCCTCCAGGAAAGAATA
AAATCCCTCAACTTGTCACTTAAAGAAATCACTACTAAGGTGTGTATGAG
TGAGAAATTCTGCAGCACCCTGAGGAAGAAAGTTAACGATATTGAAACCC
AACTACCAGCCTTGCTTGAAGCCAAAATGCATGCCATATCAGGAAACCAT
TTCTGGACGGCTAAAGACCTCACCGAGGAGATTAGATCATTAACATCAGA
GAGAGAAGGGCTGGAGGGACTCCTCAGCAAGCTGTTGGTGTTGAGTTCCA
GGAATGTCAAAAAGCTGGGAAGTGTTAAAGAAGATTACAACAGACTGAGA
AGAGAAGTGGAGCACCAGGAGACTGCCTATGAAACAAGTGTGAAGGAAAA
TACTATGAAGTACATGGAAACACTTAAGAATAAACTGTGCAGCTGCAAGT
GTCCACTGCTTGGGAAAGTGTGGGAAGCTGACTTGGAAGCTTGTCGATTG
CTTATCCAGTGCCTACAGCTCCAGGAAGCCAGGGGAAGCCTGTCTGTAGA
AGATGAGAGGCAGATGGATGACTTAGAGGGAGCTGCTCCTCCTATTCCCC
CCAGGCTCCACTCCGAGGATAAAAGGAAGACCCCTTTGAAGGTATTGGAA
GAATGGAAGACTCACCTCATCCCCTCTCTGCACTGTGCTGGAGGTGAACA
GAAAGAGGAATCTTACATCCTTTCTGCAGAACTTGGAGAAAAGTGTGAAG
ACATAGGCAAGAAGCTATTGTACTTGGAAGATCAACTTCACACAGCAATC
CACAGTCATGATGAAGATCTCATTCAGTCTCTCAGGAGGGAGCTCCAGAT
GGTGAAGGAAACTCTGCAGGCCATGATCCTGCAGCTCCAGCCAGCAAAGG
AGGCGGGAGAAAGAGAAGCTGCAGCTTCCTGCATGACAGCTGGTGTCCAC
GAAGCACAAGCCTGAGTCGAC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISEFMPGGGPQGAPAAAGGGGVSHRAGSRDCLPPAACFRRRRLARRPGYMRSSTGPGIGFLSPAVGTLFRFPGGVSGEESHHSESRARQCGLDSRGLLVRSPVSKSAAAPTVTSVRGTSAHFGIQLRGGTRLPDRLSWPCGPGSAGWQQEFAAMDSSETLDASWEAACSDGARRVRAAGSLPSAELSSNSCSPGCGPEVPPTPPGSHSAFTSSFSFIRLSLGSAGERGEAEGCPPSREAESHCQSPQEMGAKAASLDGPHEDPRCLSQPFSLLATRVSADLAQAARNSSRPERDMHSLPDMDPGSSSSLDPSLAGCGGDGSSGSGDAHSWDTLLRKWEPVLRDCLLRNRRQMEVISLRLKLQKLQEDAVENDDYDKAETLQQRLEDLEQEKISLHFQLPSRQPALSSFLGHLAAQVQAALRRGATQQASGDDTHTPLRMEPRLLEPTAQDSLHVSITRRDWLLQEKQQLQKEIEALQARMFVLEAKDQQLRREIEEQEQQLQWQGCDLTPLVGQLSLGQLQEVSKALQDTLASAGQIPFHAEPPETIRSLQERIKSLNLSLKEITTKVCMSEKFCSTLRKKVNDIETQLPALLEAKMHAISGNHFWTAKDLTEEIRSLTSEREGLEGLLSKLLVLSSRNVKKLGSVKEDYNRLRREVEHQETAYETSVKENTMKYMETLKNKLCSCKCPLLGKVWEADLEACRLLIQCLQLQEARGSLSVEDERQMDDLEGAAPPIPPRLHSEDKRKTPLKVLEEWKTHLIPSLHCAGGEQKEESYILSAELGEKCEDIGKKLLYLEDQLHTAI
HSHDEDLIQSLRRELQMVKETLQAMILQLQPAKEAGEREAAASCMTAGVH
EAQA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11880
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express MBP N terminal fusion.; ; Estimated MW of expressed protein : 139081 Daltons