cDNA Clone Rad5 (yeast)

• Plasmid: pGEX6P-Rad5
• Parent Plasmid: pGEX6P1
• Expressed: GST-Rad5
• Genbank: NP_013132
• Tag: GST
• Species: Yeast

Sequence of Insert

GGATCCATGAGTCATATTGAACAGGAAGAAAGGAAGAGGTTTTTTAACGATGACCTTGACACTTCAGAAACATCGTTAAACTTCAAATCTGAGAATAAAGAGTCGTTTTTATTTGCAAATAGTCATAATGATGATGATGATGATGTTGTTGTATCAGTGAGTGATACAACAGAAGGAGAGGGAGACCGATCTATTGTGCCAGTCAGGCGAGAAATAGAAGAAGAAGGCCAGAATCAGTTTATTACAGAGCTTTTAAGGATTATTCCAGAAATGCCAAAGGATCTTGTGATGGAACTTAATGAAAAA
TTCGGCAGTCAGGAGGAAGGGCTCTCTTTAGCATTATCACATTACTTTGA
TCACAATAGCGGGACGTCTATCAGCAAAATACCGTCTTCCCCGAATCAAC
TAAATACACTCTCAGATACCTCAAATTCAACCTTGTCCCCATCTTCATTC
CATCCTAAAAGAAGAAGAATATACGGGTTCAGAAACCAAACACGATTAGA
AGATAAAGTTACTTGGAAAAGATTTATAGGTGCTTTGCAAGTCACTGGTA
TGGCTACCAGACCCACCGTCAGGCCCTTGAAGTACGGCTCTCAGATGAAG
CTAAAGAGATCAAGTGAAGAGATTTCTGCTACTAAAGTATATGACTCACG
TGGTAGAAAGAAAGCGTCCATGGCTAGTTTGGTAAGAATTTTTGATATCC
AATATGATAGAGAAATTGGCAGAGTTTCGGAAGACATTGCTCAAATACTA
TACCCTCTTTTAAGTTCACACGAAATAAGTTTTGAAGTTACATTGATTTT
TTGTGATAATAAACGGTTGAGTATAGGTGATAGCTTTATTCTACAATTGG
ATTGCTTTTTAACATCTCTCATTTTTGAGGAACGTAATGATGGAGAATCC
TTGATGAAAAGAAGACGTACAGAGGGAGGAAATAAAAGAGAGAAAGACAA
TGGAAATTTTGGAAGAACATTGACTGAAACTGATGAAGAGCTAGAAAGCC
GCTCGAAAAGACTGGCTCTACTAAAGTTATTTGATAAATTGAGGCTAAAG
CCTATTTTGGACGAGCAGAAGGCATTAGAAAAGCATAAAATAGAGCTTAA
TAGTGACCCCGAAATCATTGATTTAGATAACGACGAGATTTGCTCTAATC
AAGTGACTGAAGTCCATAACAATCTCCGAGATACTCAGCACGAAGAAGAA
ACAATGAACTTGAATCAATTGAAAACATTTTATAAGGCCGCACAATCATC
AGAATCTTTAAAAAGTTTGCCTGAAACAGAACCTTCTCGCGATGTCTTCA
AGCTAGAACTAAGAAATTATCAAAAGCAAGGTCTTACTTGGATGCTAAGG
AGGGAGCAAGAGTTTGCCAAAGCAGCCTCTGATGGTGAGGCTTCAGAAAC
GGGTGCTAATATGATAAACCCATTATGGAAGCAGTTCAAATGGCCAAATG
ATATGTCGTGGGCAGCTCAAAATTTGCAGCAGGACCATGTAAACGTTGAA
GATGGCATATTCTTTTATGCGAACTTACATTCTGGTGAATTTTCGCTAGC
AAAACCTATATTAAAAACTATGATAAAGGGTGGCATATTATCAGATGAAA
TGGGGTTGGGTAAAACAGTGGCAGCGTATTCTTTAGTTTTATCTTGTCCT
CACGATAGTGATGTTGTTGACAAGAAACTGTTTGATATTGAGAACACAGC
AGTCTCAGATAATCTTCCAAGCACTTGGCAAGATAATAAGAAACCATATG
CTTCAAAAACAACGCTAATCGTGGTCCCAATGTCTTTGTTAACGCAGTGG
AGTAACGAGTTTACAAAAGCTAATAATTCCCCCGATATGTATCATGAGGT
GTATTATGGTGGGAATGTTTCCAGTTTGAAAACCCTATTAACCAAGACAA
AAACCCCTCCAACTGTAGTCCTTACTACATATGGTATTGTTCAAAATGAA
TGGACTAAACATTCCAAGGGAAGGATGACAGATGAGGACGTCAATATATC
TTCAGGCTTATTTTCTGTCAATTTTTATCGCATAATAATCGACGAGGGTC
ATAACATTAGAAACAGAACGACAGTTACATCTAAAGCAGTCATGGCTTTA
CAAGGCAAATGTAAATGGGTTTTAACAGGAACACCAATTATTAACAGGCT
TGACGATTTATACAGTCTGGTTAAGTTTTTAGAGTTAGATCCCTGGCGGC
AAATTAATTACTGGAAGACCTTTGTATCAACGCCTTTTGAGAGTAAAAAT
TACAAACAAGCATTTGATGTGGTGAATGCAATTCTGGAACCCGTATTGTT
AAGAAGGACAAAACAAATGAAAGATAAAGATGGTAAGCCATTAGTAGAGT
TGCCACCAAAGGAGGTCGTTATTAAAAGACTTCCCTTCAGTAAATCTCAA
GATCTTCTATACAAGTTTCTGTTGGATAAGGCTGAAGTTTCTGTTAAATC
GGGTATTGCACGCGGTGATTTATTGAAAAAGTACTCCACTATCCTTGTCC
ATATTTTAAGATTGAGGCAAGTCTGTTGCCATCCCGGTCTTATTGGGAGT
CAAGATGAGAACGATGAGGATTTATCTAAAAATAATAAATTGGTTACGGA
ACAAACGGTGGAGCTTGACTCTTTAATGCGTGTTGTTTCCGAGAGATTCG
ATAACTCATTTTCTAAGGAGGAATTAGATGCAATGATACAAAGATTAAAA
GTTAAATATCCAGACAATAAATCGTTTCAGTCCTTAGAGTGCTCCATCTG
CACAACGGAACCTATGGATTTGGACAAGGCTTTATTTACAGAATGCGGCC
ACAGTTTTTGTGAGAAATGTTTATTTGAATATATTGAGTTTCAGAACAGT
AAGAATTTGGGTTTAAAGTGCCCCAATTGCCGTAACCAAATAGACGCTTG
TCGGTTGTTGGCATTGGTACAAACGAATAGCAACTCGAAAAATTTGGAAT
TCAAACCATATTCACCAGCCTCCAAATCAAGCAAAATCACTGCTTTATTG
AAGGAGCTTCAATTGCTACAGGATAGTTCGGCAGGCGAACAAGTTGTCAT
TTTTTCCCAATTTTCTACATACTTGGATATCCTGGAGAAAGAGCTAACTC
ATACTTTCTCAAAAGATGTTGCAAAAATTTATAAATTCGATGGACGTCTC
TCATTAAAAGAAAGAACTAGTGTATTAGCAGATTTTGCCGTTAAAGACTA
TAGCAGGCAAAAAATCCTATTACTCTCGCTGAAGGCTGGTGGCGTGGGTT
TGAATCTAACGTGTGCTTCCCACGCTTATATGATGGACCCATGGTGGTCG
CCCAGTATGGAAGATCAGGCAATCGATAGACTGCATAGAATTGGCCAGAC
AAACAGCGTCAAAGTTATGAGATTTATCATACAAGATAGCATAGAAGAAA
AAATGCTACGCATTCAAGAAAAGAAGAGAACCATCGGTGAGGCCATGGAC
ACAGACGAAGACGAGAGAAGAAAAAGGAGAATTGAAGAAATCCAGATGCT
GTTTGAATAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSHIEQEERKRFFNDDLDT
SETSLNFKSENKESFLFANSHNDDDDDVVVSVSDTTEGEGDRSIVPVRRE
IEEEGQNQFITELLRIIPEMPKDLVMELNEKFGSQEEGLSLALSHYFDHN
SGTSISKIPSSPNQLNTLSDTSNSTLSPSSFHPKRRRIYGFRNQTRLEDK
VTWKRFIGALQVTGMATRPTVRPLKYGSQMKLKRSSEEISATKVYDSRGR
KKASMASLVRIFDIQYDREIGRVSEDIAQILYPLLSSHEISFEVTLIFCD
NKRLSIGDSFILQLDCFLTSLIFEERNDGESLMKRRRTEGGNKREKDNGN
FGRTLTETDEELESRSKRLALLKLFDKLRLKPILDEQKALEKHKIELNSD
PEIIDLDNDEICSNQVTEVHNNLRDTQHEEETMNLNQLKTFYKAAQSSES
LKSLPETEPSRDVFKLELRNYQKQGLTWMLRREQEFAKAASDGEASETGA
NMINPLWKQFKWPNDMSWAAQNLQQDHVNVEDGIFFYANLHSGEFSLAKP
ILKTMIKGGILSDEMGLGKTVAAYSLVLSCPHDSDVVDKKLFDIENTAVS
DNLPSTWQDNKKPYASKTTLIVVPMSLLTQWSNEFTKANNSPDMYHEVYY
GGNVSSLKTLLTKTKTPPTVVLTTYGIVQNEWTKHSKGRMTDEDVNISSG
LFSVNFYRIIIDEGHNIRNRTTVTSKAVMALQGKCKWVLTGTPIINRLDD
LYSLVKFLELDPWRQINYWKTFVSTPFESKNYKQAFDVVNAILEPVLLRR
TKQMKDKDGKPLVELPPKEVVIKRLPFSKSQDLLYKFLLDKAEVSVKSGI
ARGDLLKKYSTILVHILRLRQVCCHPGLIGSQDENDEDLSKNNKLVTEQT
VELDSLMRVVSERFDNSFSKEELDAMIQRLKVKYPDNKSFQSLECSICTT
EPMDLDKALFTECGHSFCEKCLFEYIEFQNSKNLGLKCPNCRNQIDACRL
LALVQTNSNSKNLEFKPYSPASKSSKITALLKELQLLQDSSAGEQVVIFS
QFSTYLDILEKELTHTFSKDVAKIYKFDGRLSLKERTSVLADFAVKDYSR
QKILLLSLKAGGVGLNLTCASHAYMMDPWWSPSMEDQAIDRLHRIGQTNS
VKVMRFIIQDSIEEKMLRIQEKKRTIGEAMDTDEDERRKRRIEEIQMLFE
*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11786
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 160823 Daltons