cDNA Clone Rac1

• Plasmid: pGEX4T-1-Rac1
• Parent Plasmid: pGEX4T1
• Genbank: NM_006908.4 GI:156071503
• Species: Human

Sequence of Insert

GGATCCCCGCAGGCCATCAAGTGTGTGGTGGTGGGAGACGGAGCTGTAGG
TAAAACTTGCCTACTGATCAGTTACACAACCAATGCATTTCCTGGAGAAT
ATATCCCTACTGTCTTTGACAATTATTCTGCCAATGTTATGGTAGATGGA
AAACCGGTGAATCTGGGCTTATGGGATACAGCTGGACAAGAAGATTATGA
CAGATTACGCCCCCTATCCTATCCGCAAACAGATGTGTCCTTAATTTGCT
TTTCCCTTGTGAGTCCTGCATCATTTGAAAATGTCCGTGCAAAGTGGTAT
CCTGAGGTGCGGCACCACTGTCCCAACACTCCCATCATCCTAGTGGGAAC
TAAACTTGATCTTAGGGATGATAAAGACACGATCGAGAAACTGAAGGAGA
AGAAGCTGACTCCCATCACCTATCCGCAGGGTCTAGCCATGGCTAAGGAG
ATTGGTGCCGTAAAATACCTGGAGTGCTCGGCGCTCACACAGCGAGGCCT
CAAGACAGTGTTTGACGAAGCGATCCGAGCAGTCCTCTGCCCGCCTCCCG
TGAAGAAGAGGAAGAGAAAATGCCTGCTGTTGTAAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPQAIKCVVVGDGAVGKTCLLISYT
TNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQ
TDVSLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKD
TIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIR
AVLCPPPVKKRKRKCLLL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11672
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Sequence differences compared with NM_006908.4 CDS:; t233c (F78S); t453c (silent); Michele West. Colin Watts Group.; ; Estimated MW of expressed protein : 47647 Daltons