cDNA Clone Rad5 (yeast)

• Plasmid: pGEX6P-2-Rad5 HIRAN2
• Parent Plasmid: pGEX6P2
• Expressed: GST-Rad5 HIRAN2
• Genbank: NP_013132
• Tag: GST
• Species: Yeast

Sequence of Insert

GGATCCAGAAACCAAACACGATTAGAAGATAAAGTTACTTGGAAAAGATT
TATAGGTGCTTTGCAAGTCACTGGTATGGCTACCAGACCCACCGTCAGGC
CCTTGAAGTACGGCTCTCAGATGAAGCTAAAGAGATCAAGTGAAGAGATT
TCTGCTACTAAAGTATATGACTCACGTGGTAGAAAGAAAGCGTCCATGGC
TAGTTTGGTAAGAATTTTTGATATCCAATATGATAGAGAAATTGGCAGAG
TTTCGGAAGACATTGCTCAAATACTATACCCTCTTTTAAGTTCACACGAA
ATAAGTTTTGAAGTTACATTGATTTTTTGTGATAATAAACGGTTGAGTAT
AGGTGATAGCTTTATTCTACAATTGGATTGCTTTTTAACATCTCTCATTT
TTGAGGAACGTAATGATGGAGAATCCTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSRNQTRLEDKVTWKRFIGAL
QVTGMATRPTVRPLKYGSQMKLKRSSEEISATKVYDSRGRKKASMASLVR
IFDIQYDREIGRVSEDIAQILYPLLSSHEISFEVTLIFCDNKRLSIGDSF
ILQLDCFLTSLIFEERNDGES*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11597
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 42937 Daltons