cDNA Clone Rad1 (yeast)

• Plasmid: pACT2.6 Rad1
• Parent Plasmid: pACT2.6
• Genbank: M15435.1 GI:172324
• Species: Yeast

Sequence of Insert

ggatccATGTCTCAGTTATTTTATCAGGGCGACTCTGATGATGAGCTCCA
GGAGGAACTTACGAGGCAGACAACTCAAGCATCTCAAAGTTCTAAAATTA
AAAATGAAGATGAACCCGACGACTCCAATCATCTTAATGAGGTGGAAAAT
GAAGATAGCAAAGTTTTAGATGACGATGCAGTGTTATACCCTCTTATACC
TAATGAGCCAGATGACATAGAAACGTCTAAGCCCAATATTAACGATATTA
GGCCAGTTGATATTCAATTGACTTTACCATTGCCGTTTCAGCAAAAAGTG
GTAGAGAATTCATTAATTACTGAAGATGCATTAATCATAATGGGGAAAGG
ACTAGGATTGCTTGATATTGTGGCCAATTTATTGCATGTTTTAGCTACAC
CAACATCCATTAACGGACAACTAAAGCGAGCGCTCGTCCTAGTGTTGAAT
GCAAAACCTATAGATAATGTAAGAATCAAGGAGGCCTTAGAAGAGCTGTC
GTGGTTCTCTAATACTGGGAAGGACGACGACGATACTGCTGTCGAGAGCG
ATGATGAACTTTTTGAAAGGCCTTTTAACGTAGTTACCGCGGACTCGCTG
AGCATTGAAAAGAGAAGAAAGCTATATATTTCTGGCGGAATCTTGAGCAT
TACTTCTAGAATTCTCATTGTGGATCTCTTATCCGGCATTGTTCACCCAA
ATAGGGTTACGGGTATGCTGGTATTGAATGCAGACTCACTTCGACATAAT
TCGAATGAATCGTTTATATTAGAGATTTACAGGTCTAAAAATACTTGGGG
TTTTATTAAAGCCTTTTCTGAAGCACCAGAGACGTTTGTCATGGAATTTT
CACCCCTCAGGACGAAAATGAAAGAATTACGGCTAAAGAACGTTTTGCTA
TGGCCGAGGTTCAGGGTAGAGGTCTCTTCCTGTTTGAATGCCACTAATAA
GACGTCACACAATAAAGTCATTGAAGTCAAGGTCTCCTTAACAAATTCCA
TGTCTCAGATACAGTTTGGCTTGATGGAATGTTTGAAAAAATGTATTGCT
GAGTTAAGCAGAAAAAATCCTGAACTAGCTCTGGACTGGTGGAATATGGA
AAATGTCCTGGATATAAACTTTATCAGGTCAATTGACTCGGTGATGGTGC
CGAACTGGCACCGAATTTCTTATGAATCAAAACAACTGGTTAAGGATATA
AGATTCCTACGCCACCTTTTAAAGATGCTCGTAACTTCAGACGCAGTTGA
CTTTTTTGGAGAGATTCAATTAAGTTTGGATGCCAATAAACCGTCAGTAT
CCCGAAAATACAGCGAATCACCGTGGCTATTGGTCGATGAGGCACAATTA
GTCATATCGTATGCGAAGAAAAGAATATTTTACAAAAATGAATATACTTT
AGAAGAAAATCCAAAATGGGAACAACTTATTCATATATTACATGATATTT
CACATGAGAGAATGACCAATCACCTTCAGGGGCCTACTTTAGTTGCCTGT
TCCGACAACCTTACATGTTTAGAACTAGCAAAGGTCTTGAATGCCTCAAA
CAAAAAAAGAGGAGTACGTCAAGTGCTTCTGAATAAATTGAAATGGTACA
GAAAACAGAGGGAGGAAACGAAAAAATTGGTCAAAGAAGTGCAAAGTCAG
GACACTTTTCCAGAGAATGCAACATTAAATGTAAGCTCGACATTTTCCAA
AGAACAAGTGACCACGAAAAGAAGAAGGACAAGAGGTGCTTCACAAGTTG
CGGCCGTTGAAAAGCTAAGGAATGCAGGTACCAATGTAGATATGGAGGTG
GTTTTTGAGGATCATAAGTTATCTGAAGAAATTAAGAAGGGAAGCGGTGA
TGATTTGGATGACGGTCAGGAAGAAAATGCCGCAAACGATTCAAAGATTT
TTGAAATACAAGAACAGGAAAATGAAATCCTTATCGATGATGGGGATGCT
GAATTTGACAACGGAGAATTAGAGTATGTGGGCGACCTTCCGCAGCACAT
CACAACCCATTTCAATAAGGATTTATGGGCAGAACATTGCAACGAGTATG
AATATGTTGATCGTCAGGACGAAATTTTAATCTCTACGTTTAAAAGTCTC
AATGACAATTGCTCATTGCAGGAGATGATGCCCTCTTACATTATAATGTT
TGAACCAGATATATCGTTTATCAGGCAGATTGAAGTTTATAAGGCCATAG
TGAAGGATTTGCAACCAAAAGTATACTTCATGTACTACGGTGAAAGTATT
GAAGAGCAAAGTCATTTGACTGCTATCAAGAGAGAGAAAGATGCTTTCAC
AAAGTTGATTAGAGAGAATGCAAATCTGTCCCATCACTTTGAAACGAATG
AAGATCTTTCTCACTACAAAAATTTAGCTGAAAGGAAGTTGAAGCTTTCA
AAATTACGAAAATCTAATACCAGAAATGCGGGTGGGCAGCAGGGATTCCA
TAATCTTACTCAGGATGTCGTCATTGTGGATACACGTGAGTTTAATGCCT
CATTACCAGGCTTACTCTACCGATATGGCATAAGGGTTATTCCTTGTATG
TTGACAGTCGGCGATTATGTGATAACTCCTGATATTTGTCTCGAAAGAAA
ATCGATTTCTGACTTAATTGGGTCATTACAGAATAACAGATTAGCCAACC
AATGTAAAAAAATGTTAAAATACTATGCATATCCGACACTATTGATTGAG
TTTGATGAAGGACAGTCGTTTTCTTTAGAACCTTTTAGTGAACGTAGAAA
TTATAAGAATAAAGACATATCAACTGTTCATCCTATATCAAGCAAGTTAT
CCCAGGATGAAATTCAGCTAAAACTAGCCAAATTAGTATTGCGGTTTCCC
ACTTTAAAGATTATATGGTCTTCCTCACCCCTGCAAACTGTAAATATAAT
CCTAGAGTTGAAATTAGGACGTGAGCAACCTGACCCTAGTAATGCAGTTA
TATTGGGAACGAATAAAGTTAGATCGGATTTTAATAGCACTGCAAAGGGC
CTGAAGGATGGTGATAACGAGTCTAAATTCAAGAGACTGTTGAATGTTCC
TGGAGTGTCAAAAATTGATTATTTCAATCTCCGCAAAAAGATCAAGAGCT
TCAATAAGCTTCAAAAGCTTTCATGGAATGAGATTAATGAACTTATTAAT
GACGAAGATTTGACGGATAGAATATACTACTTCTTGAGAACAGAAAAAGA
AGAACAAGAACAAGAGTCAACAGATGAAAATCTTGAATCTCCTGGTAAGA
CCACTGATGATAACGCTTTACATGATCATCATAATGATGTTCCTGAAGCA
CCTGTGTAActcgag

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLI
TTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYAS
LGGHMAMGGSMSQLFYQGDSDDELQEELTRQTTQASQSSKIKNEDEPDDS
NHLNEVENEDSKVLDDDAVLYPLIPNEPDDIETSKPNINDIRPVDIQLTL
PLPFQQKVVENSLITEDALIIMGKGLGLLDIVANLLHVLATPTSINGQLK
RALVLVLNAKPIDNVRIKEALEELSWFSNTGKDDDDTAVESDDELFERPF
NVVTADSLSIEKRRKLYISGGILSITSRILIVDLLSGIVHPNRVTGMLVL
NADSLRHNSNESFILEIYRSKNTWGFIKAFSEAPETFVMEFSPLRTKMKE
LRLKNVLLWPRFRVEVSSCLNATNKTSHNKVIEVKVSLTNSMSQIQFGLM
ECLKKCIAELSRKNPELALDWWNMENVLDINFIRSIDSVMVPNWHRISYE
SKQLVKDIRFLRHLLKMLVTSDAVDFFGEIQLSLDANKPSVSRKYSESPW
LLVDEAQLVISYAKKRIFYKNEYTLEENPKWEQLIHILHDISHERMTNHL
QGPTLVACSDNLTCLELAKVLNASNKKRGVRQVLLNKLKWYRKQREETKK
LVKEVQSQDTFPENATLNVSSTFSKEQVTTKRRRTRGASQVAAVEKLRNA
GTNVDMEVVFEDHKLSEEIKKGSGDDLDDGQEENAANDSKIFEIQEQENE
ILIDDGDAEFDNGELEYVGDLPQHITTHFNKDLWAEHCNEYEYVDRQDEI
LISTFKSLNDNCSLQEMMPSYIIMFEPDISFIRQIEVYKAIVKDLQPKVY
FMYYGESIEEQSHLTAIKREKDAFTKLIRENANLSHHFETNEDLSHYKNL
AERKLKLSKLRKSNTRNAGGQQGFHNLTQDVVIVDTREFNASLPGLLYRY
GIRVIPCMLTVGDYVITPDICLERKSISDLIGSLQNNRLANQCKKMLKYY
AYPTLLIEFDEGQSFSLEPFSERRNYKNKDISTVHPISSKLSQDEIQLKL
AKLVLRFPTLKIIWSSSPLQTVNIILELKLGREQPDPSNAVILGTNKVRS
DFNSTAKGLKDGDNESKFKRLLNVPGVSKIDYFNLRKKIKSFNKLQKLSW
NEINELINDEDLTDRIYYFLRTEKEEQEQESTDENLESPGKTTDDNALHD
HHNDVPEAPV*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11466
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All Rad1 plasmids MUST be grown at 30 degrees C or less to prevent recombination.