cDNA Clone Rad1 (yeast)

• Plasmid: pFastBacHTb Rad1
• Parent Plasmid: pFastBac HTb
• Species: Yeast

Sequence of Insert

ggatccATGTCTCAGTTATTTTATCAGGGCGACTCTGATGATGAGCTCCAGGAGGAACTTACGAGGCAGACAACTCAAGCATCTCAAAGTTCTAAAATTAAAAATGAAGATGAACCCGACGACTCCAATCATCTTAATGAGGTGGAAAATGAAGATAGCAAAGTTTTAGATGACGATGCAGTGTTATACCCTCTTATACCTAATGAGCCAGATGACATAGAAACGTCTAAGCCCAATATTAACGATATTAGGCCAGTTGATATTCAATTGACTTTACCATTGCCGTTTCAGCAAAAAGTGGTAGAGAATTCATTAATTACTGAAGATGCATTAATCATAATGGGGAAAGGACTAGG
ATTGCTTGATATTGTGGCCAATTTATTGCATGTTTTAGCTACACCAACAT
CCATTAACGGACAACTAAAGCGAGCGCTCGTCCTAGTGTTGAATGCAAAA
CCTATAGATAATGTAAGAATCAAGGAGGCCTTAGAAGAGCTGTCGTGGTT
CTCTAATACTGGGAAGGACGACGACGATACTGCTGTCGAGAGCGATGATG
AACTTTTTGAAAGGCCTTTTAACGTAGTTACCGCGGACTCGCTGAGCATT
GAAAAGAGAAGAAAGCTATATATTTCTGGCGGAATCTTGAGCATTACTTC
TAGAATTCTCATTGTGGATCTCTTATCCGGCATTGTTCACCCAAATAGGG
TTACGGGTATGCTGGTATTGAATGCAGACTCACTTCGACATAATTCGAAT
GAATCGTTTATATTAGAGATTTACAGGTCTAAAAATACTTGGGGTTTTAT
TAAAGCCTTTTCTGAAGCACCAGAGACGTTTGTCATGGAATTTTCACCCC
TCAGGACGAAAATGAAAGAATTACGGCTAAAGAACGTTTTGCTATGGCCG
AGGTTCAGGGTAGAGGTCTCTTCCTGTTTGAATGCCACTAATAAGACGTC
ACACAATAAAGTCATTGAAGTCAAGGTCTCCTTAACAAATTCCATGTCTC
AGATACAGTTTGGCTTGATGGAATGTTTGAAAAAATGTATTGCTGAGTTA
AGCAGAAAAAATCCTGAACTAGCTCTGGACTGGTGGAATATGGAAAATGT
CCTGGATATAAACTTTATCAGGTCAATTGACTCGGTGATGGTGCCGAACT
GGCACCGAATTTCTTATGAATCAAAACAACTGGTTAAGGATATAAGATTC
CTACGCCACCTTTTAAAGATGCTCGTAACTTCAGACGCAGTTGACTTTTT
TGGAGAGATTCAATTAAGTTTGGATGCCAATAAACCGTCAGTATCCCGAA
AATACAGCGAATCACCGTGGCTATTGGTCGATGAGGCACAATTAGTCATA
TCGTATGCGAAGAAAAGAATATTTTACAAAAATGAATATACTTTAGAAGA
AAATCCAAAATGGGAACAACTTATTCATATATTACATGATATTTCACATG
AGAGAATGACCAATCACCTTCAGGGGCCTACTTTAGTTGCCTGTTCCGAC
AACCTTACATGTTTAGAACTAGCAAAGGTCTTGAATGCCTCAAACAAAAA
AAGAGGAGTACGTCAAGTGCTTCTGAATAAATTGAAATGGTACAGAAAAC
AGAGGGAGGAAACGAAAAAATTGGTCAAAGAAGTGCAAAGTCAGGACACT
TTTCCAGAGAATGCAACATTAAATGTAAGCTCGACATTTTCCAAAGAACA
AGTGACCACGAAAAGAAGAAGGACAAGAGGTGCTTCACAAGTTGCGGCCG
TTGAAAAGCTAAGGAATGCAGGTACCAATGTAGATATGGAGGTGGTTTTT
GAGGATCATAAGTTATCTGAAGAAATTAAGAAGGGAAGCGGTGATGATTT
GGATGACGGTCAGGAAGAAAATGCCGCAAACGATTCAAAGATTTTTGAAA
TACAAGAACAGGAAAATGAAATCCTTATCGATGATGGGGATGCTGAATTT
GACAACGGAGAATTAGAGTATGTGGGCGACCTTCCGCAGCACATCACAAC
CCATTTCAATAAGGATTTATGGGCAGAACATTGCAACGAGTATGAATATG
TTGATCGTCAGGACGAAATTTTAATCTCTACGTTTAAAAGTCTCAATGAC
AATTGCTCATTGCAGGAGATGATGCCCTCTTACATTATAATGTTTGAACC
AGATATATCGTTTATCAGGCAGATTGAAGTTTATAAGGCCATAGTGAAGG
ATTTGCAACCAAAAGTATACTTCATGTACTACGGTGAAAGTATTGAAGAG
CAAAGTCATTTGACTGCTATCAAGAGAGAGAAAGATGCTTTCACAAAGTT
GATTAGAGAGAATGCAAATCTGTCCCATCACTTTGAAACGAATGAAGATC
TTTCTCACTACAAAAATTTAGCTGAAAGGAAGTTGAAGCTTTCAAAATTA
CGAAAATCTAATACCAGAAATGCGGGTGGGCAGCAGGGATTCCATAATCT
TACTCAGGATGTCGTCATTGTGGATACACGTGAGTTTAATGCCTCATTAC
CAGGCTTACTCTACCGATATGGCATAAGGGTTATTCCTTGTATGTTGACA
GTCGGCGATTATGTGATAACTCCTGATATTTGTCTCGAAAGAAAATCGAT
TTCTGACTTAATTGGGTCATTACAGAATAACAGATTAGCCAACCAATGTA
AAAAAATGTTAAAATACTATGCATATCCGACACTATTGATTGAGTTTGAT
GAAGGACAGTCGTTTTCTTTAGAACCTTTTAGTGAACGTAGAAATTATAA
GAATAAAGACATATCAACTGTTCATCCTATATCAAGCAAGTTATCCCAGG
ATGAAATTCAGCTAAAACTAGCCAAATTAGTATTGCGGTTTCCCACTTTA
AAGATTATATGGTCTTCCTCACCCCTGCAAACTGTAAATATAATCCTAGA
GTTGAAATTAGGACGTGAGCAACCTGACCCTAGTAATGCAGTTATATTGG
GAACGAATAAAGTTAGATCGGATTTTAATAGCACTGCAAAGGGCCTGAAG
GATGGTGATAACGAGTCTAAATTCAAGAGACTGTTGAATGTTCCTGGAGT
GTCAAAAATTGATTATTTCAATCTCCGCAAAAAGATCAAGAGCTTCAATA
AGCTTCAAAAGCTTTCATGGAATGAGATTAATGAACTTATTAATGACGAA
GATTTGACGGATAGAATATACTACTTCTTGAGAACAGAAAAAGAAGAACA
AGAACAAGAGTCAACAGATGAAAATCTTGAATCTCCTGGTAAGACCACTG
ATGATAACGCTTTACATGATCATCATAATGATGTTCCTGAAGCACCTGTG
TAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMSQLFYQGDSDDELQEELTRQT
TQASQSSKIKNEDEPDDSNHLNEVENEDSKVLDDDAVLYPLIPNEPDDIE
TSKPNINDIRPVDIQLTLPLPFQQKVVENSLITEDALIIMGKGLGLLDIV
ANLLHVLATPTSINGQLKRALVLVLNAKPIDNVRIKEALEELSWFSNTGK
DDDDTAVESDDELFERPFNVVTADSLSIEKRRKLYISGGILSITSRILIV
DLLSGIVHPNRVTGMLVLNADSLRHNSNESFILEIYRSKNTWGFIKAFSE
APETFVMEFSPLRTKMKELRLKNVLLWPRFRVEVSSCLNATNKTSHNKVI
EVKVSLTNSMSQIQFGLMECLKKCIAELSRKNPELALDWWNMENVLDINF
IRSIDSVMVPNWHRISYESKQLVKDIRFLRHLLKMLVTSDAVDFFGEIQL
SLDANKPSVSRKYSESPWLLVDEAQLVISYAKKRIFYKNEYTLEENPKWE
QLIHILHDISHERMTNHLQGPTLVACSDNLTCLELAKVLNASNKKRGVRQ
VLLNKLKWYRKQREETKKLVKEVQSQDTFPENATLNVSSTFSKEQVTTKR
RRTRGASQVAAVEKLRNAGTNVDMEVVFEDHKLSEEIKKGSGDDLDDGQE
ENAANDSKIFEIQEQENEILIDDGDAEFDNGELEYVGDLPQHITTHFNKD
LWAEHCNEYEYVDRQDEILISTFKSLNDNCSLQEMMPSYIIMFEPDISFI
RQIEVYKAIVKDLQPKVYFMYYGESIEEQSHLTAIKREKDAFTKLIRENA
NLSHHFETNEDLSHYKNLAERKLKLSKLRKSNTRNAGGQQGFHNLTQDVV
IVDTREFNASLPGLLYRYGIRVIPCMLTVGDYVITPDICLERKSISDLIG
SLQNNRLANQCKKMLKYYAYPTLLIEFDEGQSFSLEPFSERRNYKNKDIS
TVHPISSKLSQDEIQLKLAKLVLRFPTLKIIWSSSPLQTVNIILELKLGR
EQPDPSNAVILGTNKVRSDFNSTAKGLKDGDNESKFKRLLNVPGVSKIDY
FNLRKKIKSFNKLQKLSWNEINELINDEDLTDRIYYFLRTEKEEQEQEST
DENLESPGKTTDDNALHDHHNDVPEAPV*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU11443
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 129739 Daltons; ; All Rad1 plasmids MUST be grown at 30 degrees C or less to prevent recombination.