cDNA Clone Flotillin 1

• Plasmid: pGEX6P-1-Flotillin 1
• Parent Plasmid: pGEX6P1
• Expressed: GST-Flotillin
• Genbank: NM_005803.2 GI:6552331
• Tag: GST
• Species: Human

Sequence of Insert

gaattcatgtttttcacttgtggcccaaatgaggccatggtggtctccgggttctgccgaagccccccagtcatggtggctggagggcgtgtctttgtcctgccctgcatccaacagatccagaggatctctctcaacacactgaccctcaatgtcaagagtgaaaaggtttacactcgccatggggtccccatctcagtcactggcattgcccaggtaaaaatccaggggcagaacaaggagatgttggcggccgcctgtcagatgttcctggggaagacggaggctgagattgcccacattgcc
ctggagacgttagagggccaccagagggccatcatggcccacatgactgt
ggaggagatctataaggacaggcagaaattctcagaacaggttttcaaag
tggcctcctcagacctggtcaacatgggcatcagtgtggttagctacact
ctgaaggacattcacgatgaccaggactatttgcactctttggggaaggc
tcgaacagctcaagtccaaaaagatgcacggattggagaagcagaggcca
agagagatgctgggatccgggaagctaaagccaagcaggaaaaggtgtct
gctcagtacctgagtgagatcgagatggccaaggcacagagagattacga
actgaagaaggccgcctatgacatcgaggtcaacacccgccgagcacagg
ctgacctggcctatcagcttcaggtggccaagactaagcagcagattgag
gagcagcgggtgcaggtgcaggtggtggagcgggcccagcaggtggcagt
gcaggagcaggagatcgcccggcgggagaaggagctggaggcccgggtgc
ggaagccagcggaagcggagcgctacaagctggagcgcctagccgaggca
gagaagtcccaactaattatgcaggcggaggcagaagccgcgtctgtgcg
gatgcgtggggaagctgaggcctttgccataggggcccgagcccgagccg
aggctgagcagatggccaagaaggcagaagccttccagctgtaccaagag
gctgctcagctggacatgctgctagagaagctgccccaggtggcagagga
gatcagtggtcccttgacttcagccaataagatcacactggtgtccagcg
gcagtgggaccatgggggcagccaaagtgactggggaagtactggacatt
ctaactcgcctgccagagagtgtggaaagactcacaggcgtgagcatctc
ccaggtgaatcacaagcctttgagaacagcctgagaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMFFTCGPNEAMVVSGF
CRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVT
GIAQVKIQGQNKEMLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMT
VEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGK
ARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDY
ELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVA
VQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASV
RMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAE
EISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSI
SQVNHKPLRTA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11439
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 74548 Daltons