cDNA Clone Rad1 (yeast)

• Plasmid: pETDuet 6His-Rad1
• Parent Plasmid: pETDuet1
• Tag: 6His
• Species: Yeast

Sequence of Insert

ggatccgATGTCTCAGTTATTTTATCAGGGCGACTCTGATGATGAGCTCCAGGAGGAACTTACGAGGCAGACAACTCAAGCATCTCAAAGTTCTAAAATTAAAAATGAAGATGAACCCGACGACTCCAATCATCTTAATGAGGTGGAAAATGAAGATAGCAAAGTTTTAGATGACGATGCAGTGTTATACCCTCTTATACCTAATGAGCCAGATGACATAGAAACGTCTAAGCCCAATATTAACGATATTAGGCCAGTTGATATTCAATTGACTTTACCATTGCCGTTTCAGCAAAAAGTGGTAGAGAATTCATTAATTACTGAAGATGCATTAATCATAATGGGGAAAGGACTAGG
ATTGCTTGATATTGTGGCCAATTTATTGCATGTTTTAGCTACACCAACAT
CCATTAACGGACAACTAAAGCGAGCGCTCGTCCTAGTGTTGAATGCAAAA
CCTATAGATAATGTAAGAATCAAGGAGGCCTTAGAAGAGCTGTCGTGGTT
CTCTAATACTGGGAAGGACGACGACGATACTGCTGTCGAGAGCGATGATG
AACTTTTTGAAAGGCCTTTTAACGTAGTTACCGCGGACTCGCTGAGCATT
GAAAAGAGAAGAAAGCTATATATTTCTGGCGGAATCTTGAGCATTACTTC
TAGAATTCTCATTGTGGATCTCTTATCCGGCATTGTTCACCCAAATAGGG
TTACGGGTATGCTGGTATTGAATGCAGACTCACTTCGACATAATTCGAAT
GAATCGTTTATATTAGAGATTTACAGGTCTAAAAATACTTGGGGTTTTAT
TAAAGCCTTTTCTGAAGCACCAGAGACGTTTGTCATGGAATTTTCACCCC
TCAGGACGAAAATGAAAGAATTACGGCTAAAGAACGTTTTGCTATGGCCG
AGGTTCAGGGTAGAGGTCTCTTCCTGTTTGAATGCCACTAATAAGACGTC
ACACAATAAAGTCATTGAAGTCAAGGTCTCCTTAACAAATTCCATGTCTC
AGATACAGTTTGGCTTGATGGAATGTTTGAAAAAATGTATTGCTGAGTTA
AGCAGAAAAAATCCTGAACTAGCTCTGGACTGGTGGAATATGGAAAATGT
CCTGGATATAAACTTTATCAGGTCAATTGACTCGGTGATGGTGCCGAACT
GGCACCGAATTTCTTATGAATCAAAACAACTGGTTAAGGATATAAGATTC
CTACGCCACCTTTTAAAGATGCTCGTAACTTCAGACGCAGTTGACTTTTT
TGGAGAGATTCAATTAAGTTTGGATGCCAATAAACCGTCAGTATCCCGAA
AATACAGCGAATCACCGTGGCTATTGGTCGATGAGGCACAATTAGTCATA
TCGTATGCGAAGAAAAGAATATTTTACAAAAATGAATATACTTTAGAAGA
AAATCCAAAATGGGAACAACTTATTCATATATTACATGATATTTCACATG
AGAGAATGACCAATCACCTTCAGGGGCCTACTTTAGTTGCCTGTTCCGAC
AACCTTACATGTTTAGAACTAGCAAAGGTCTTGAATGCCTCAAACAAAAA
AAGAGGAGTACGTCAAGTGCTTCTGAATAAATTGAAATGGTACAGAAAAC
AGAGGGAGGAAACGAAAAAATTGGTCAAAGAAGTGCAAAGTCAGGACACT
TTTCCAGAGAATGCAACATTAAATGTAAGCTCGACATTTTCCAAAGAACA
AGTGACCACGAAAAGAAGAAGGACAAGAGGTGCTTCACAAGTTGCGGCCG
TTGAAAAGCTAAGGAATGCAGGTACCAATGTAGATATGGAGGTGGTTTTT
GAGGATCATAAGTTATCTGAAGAAATTAAGAAGGGAAGCGGTGATGATTT
GGATGACGGTCAGGAAGAAAATGCCGCAAACGATTCAAAGATTTTTGAAA
TACAAGAACAGGAAAATGAAATCCTTATCGATGATGGGGATGCTGAATTT
GACAACGGAGAATTAGAGTATGTGGGCGACCTTCCGCAGCACATCACAAC
CCATTTCAATAAGGATTTATGGGCAGAACATTGCAACGAGTATGAATATG
TTGATCGTCAGGACGAAATTTTAATCTCTACGTTTAAAAGTCTCAATGAC
AATTGCTCATTGCAGGAGATGATGCCCTCTTACATTATAATGTTTGAACC
AGATATATCGTTTATCAGGCAGATTGAAGTTTATAAGGCCATAGTGAAGG
ATTTGCAACCAAAAGTATACTTCATGTACTACGGTGAAAGTATTGAAGAG
CAAAGTCATTTGACTGCTATCAAGAGAGAGAAAGATGCTTTCACAAAGTT
GATTAGAGAGAATGCAAATCTGTCCCATCACTTTGAAACGAATGAAGATC
TTTCTCACTACAAAAATTTAGCTGAAAGGAAGTTGAAGCTTTCAAAATTA
CGAAAATCTAATACCAGAAATGCGGGTGGGCAGCAGGGATTCCATAATCT
TACTCAGGATGTCGTCATTGTGGATACACGTGAGTTTAATGCCTCATTAC
CAGGCTTACTCTACCGATATGGCATAAGGGTTATTCCTTGTATGTTGACA
GTCGGCGATTATGTGATAACTCCTGATATTTGTCTCGAAAGAAAATCGAT
TTCTGACTTAATTGGGTCATTACAGAATAACAGATTAGCCAACCAATGTA
AAAAAATGTTAAAATACTATGCATATCCGACACTATTGATTGAGTTTGAT
GAAGGACAGTCGTTTTCTTTAGAACCTTTTAGTGAACGTAGAAATTATAA
GAATAAAGACATATCAACTGTTCATCCTATATCAAGCAAGTTATCCCAGG
ATGAAATTCAGCTAAAACTAGCCAAATTAGTATTGCGGTTTCCCACTTTA
AAGATTATATGGTCTTCCTCACCCCTGCAAACTGTAAATATAATCCTAGA
GTTGAAATTAGGACGTGAGCAACCTGACCCTAGTAATGCAGTTATATTGG
GAACGAATAAAGTTAGATCGGATTTTAATAGCACTGCAAAGGGCCTGAAG
GATGGTGATAACGAGTCTAAATTCAAGAGACTGTTGAATGTTCCTGGAGT
GTCAAAAATTGATTATTTCAATCTCCGCAAAAAGATCAAGAGCTTCAATA
AGCTTCAAAAGCTTTCATGGAATGAGATTAATGAACTTATTAATGACGAA
GATTTGACGGATAGAATATACTACTTCTTGAGAACAGAAAAAGAAGAACA
AGAACAAGAGTCAACAGATGAAAATCTTGAATCTCCTGGTAAGACCACTG
ATGATAACGCTTTACATGATCATCATAATGATGTTCCTGAAGCACCTGTG
TAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSQDPMSQLFYQGDSDDELQEELTRQTTQASQSSKIKNEDE
PDDSNHLNEVENEDSKVLDDDAVLYPLIPNEPDDIETSKPNINDIRPVDI
QLTLPLPFQQKVVENSLITEDALIIMGKGLGLLDIVANLLHVLATPTSIN
GQLKRALVLVLNAKPIDNVRIKEALEELSWFSNTGKDDDDTAVESDDELF
ERPFNVVTADSLSIEKRRKLYISGGILSITSRILIVDLLSGIVHPNRVTG
MLVLNADSLRHNSNESFILEIYRSKNTWGFIKAFSEAPETFVMEFSPLRT
KMKELRLKNVLLWPRFRVEVSSCLNATNKTSHNKVIEVKVSLTNSMSQIQ
FGLMECLKKCIAELSRKNPELALDWWNMENVLDINFIRSIDSVMVPNWHR
ISYESKQLVKDIRFLRHLLKMLVTSDAVDFFGEIQLSLDANKPSVSRKYS
ESPWLLVDEAQLVISYAKKRIFYKNEYTLEENPKWEQLIHILHDISHERM
TNHLQGPTLVACSDNLTCLELAKVLNASNKKRGVRQVLLNKLKWYRKQRE
ETKKLVKEVQSQDTFPENATLNVSSTFSKEQVTTKRRRTRGASQVAAVEK
LRNAGTNVDMEVVFEDHKLSEEIKKGSGDDLDDGQEENAANDSKIFEIQE
QENEILIDDGDAEFDNGELEYVGDLPQHITTHFNKDLWAEHCNEYEYVDR
QDEILISTFKSLNDNCSLQEMMPSYIIMFEPDISFIRQIEVYKAIVKDLQ
PKVYFMYYGESIEEQSHLTAIKREKDAFTKLIRENANLSHHFETNEDLSH
YKNLAERKLKLSKLRKSNTRNAGGQQGFHNLTQDVVIVDTREFNASLPGL
LYRYGIRVIPCMLTVGDYVITPDICLERKSISDLIGSLQNNRLANQCKKM
LKYYAYPTLLIEFDEGQSFSLEPFSERRNYKNKDISTVHPISSKLSQDEI
QLKLAKLVLRFPTLKIIWSSSPLQTVNIILELKLGREQPDPSNAVILGTN
KVRSDFNSTAKGLKDGDNESKFKRLLNVPGVSKIDYFNLRKKIKSFNKLQ
KLSWNEINELINDEDLTDRIYYFLRTEKEEQEQESTDENLESPGKTTDDN
ALHDHHNDVPEAPV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11419
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All Rad1 plasmids MUST be grown at 30 degrees C or less to prevent recombination.