cDNA Clone SIK3 (Long)

• Synonyms: SIK3
• Plasmid: pGEX6P-1-QSK 2-380 (kinase domain)
• Parent Plasmid: pGEX6P1
• Expressed: GST-QSK 2-380 (kinase domain)
• Genbank: NM_025164 covers residues 864 – 1369
• Tag: GST
• Species: Human

Sequence of Insert

ggatccGCGGCGGCGGCGGCGAGCGGAGCTGGCGGGGCTGCCGGGGCCGGGACTGGGGGAGCCGGGCCCGCGGGCCGCCTGCTGCCTCCGCCCGCGCCGGGGTCCCCAGCCGCCCCCGCTGCCGTGTCCCCTGCGGCCGGCCAGCCGCGTCCC
CCAGCCCCGGCCTCCCGCGGACCCATGCCCGCCCGTATCGGCTACTACGA
GATCGACCGCACCATCGGCAAGGGCAACTTCGCGGTGGTCAAGCGGGCCA
CGCACCTCGTCACCAAGGCCAAGGTTGCTATCAAGATCATAGATAAGACC
CAGCTGGATGAAGAAAACTTGAAGAAGATTTTCCGGGAAGTTCAAATTAT
GAAGATGCTTTGCCACCCCCATATCATCAGGCTCTACCAGGTTATGGAGA
CAGAACGGATGATTTATCTGGTGACAGAATATGCTAGTGGAGGGGAAATA
TTTGACCACCTGGTGGCCCATGGTAGAATGGCAGAAAAGGAGGCACGTCG
GAAGTTCAAACAGATCGTCACAGCTGTCTATTTTTGTCACTGTCGGAACA
TTGTTCATCGTGATTTAAAAGCTGAAAATTTACTTCTGGATGCCAATCTG
AATATCAAAATAGCAGATTTTGGTTTCAGTAACCTCTTCACTCCTGGGCA
GCTGCTGAAGACCTGGTGTGGCAGCCCTCCCTATGCTGCACCTGAACTCT
TTGAAGGAAAAGAATATGATGGGCCCAAAGTGGACATCTGGAGCCTTGGA
GTTGTCCTCTACGTGCTTGTGTGCGGTGCCCTGCCATTTGATGGAAGCAC
ACTGCAGAATCTGCGGGCCCGCGTGCTGAGTGGAAAGTTCCGCATCCCAT
TTTTTATGTCCACAGAATGTGAGCATTTGATCCGCCATATGTTGGTGTTA
GATCCCAATAAGCGCCTCTCCATGGAGCAGATCTGCAAGCACAAGTGGAT
GAAGCTAGGGGACGCCGATCCCAACTTTGACAGGTTAATAGCTGAATGCC
AACAACTAAAGGAAGAAAGACAGGTGGACCCCCTGAATGAGGATGTCCTC
TTGGCCATGGAGGACATGGGACTGGACAAAGAACAGACACTGCAGTCATT
AAGATCAGATGCCTATGATCACTATAGTGCAATCTACAGCTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAAAAASGAGGAAGAGTGGA
GPAGRLLPPPAPGSPAAPAAVSPAAGQPRPPAPASRGPMPARIGYYEIDR
TIGKGNFAVVKRATHLVTKAKVAIKIIDKTQLDEENLKKIFREVQIMKML
CHPHIIRLYQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARRKFK
QIVTAVYFCHCRNIVHRDLKAENLLLDANLNIKIADFGFSNLFTPGQLLK
TWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTLQN
LRARVLSGKFRIPFFMSTECEHLIRHMLVLDPNKRLSMEQICKHKWMKLG
DADPNFDRLIAECQQLKEERQVDPLNEDVLLAMEDMGLDKEQTLQSLRSD
AYDHYSAIYS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5106
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Estimated MW of expressed protein : 69120 Daltons; ; The sequence of this clone refers to an older reference sequence with an additional 59 amino acids at the N terminus.