cDNA Clone DGCR8

• Plasmid: pEGFP-C2-TAP DGCR8 FL
• Parent Plasmid: pEGFP TAP
• Expressed: GFP-TAP-DGCR8 FL
• Genbank: CR456356
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT
CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG
GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACC
ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA
CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT
TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC
TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGG
CGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGG
ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAC
GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA
GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC
AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGTCCGGCCGGACTCAGATCTCGAGCTCAAGCTTC
GATATCGCCCTTGCCACCATGGACACAAGTGCCCACGATGAAGCCGTAGA
CAACAAATTCAACAAAGAACAACAAAACGCGTTCTATGAGATCTTACATT
TACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCCAAAGTTTAAAA
GATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAA
TGATGCTCAGGCGCCGAAAGTAGACAACAAATTCAACAAAGAACGACAAA
ACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGA
AACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCT
TTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCGAAAGTAGACG
CGAGTTGGTCAGCGGGTGCTAGCGAGAACTTGTACTTCCAGGGCGGAAGA
TCGATGGAAAAGAGAAGATGGAAAAAGAATTTCATAGCCGTCTCAGCAGC
CAACCGCTTTAAGAAAATCTCATCCTCCGGGGCACTTGATTATGATATTC
CAACTGCTCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCTATGGAGACA
GATGAGAGCCCCTCTCCGCTCCCGTGTGGGCCCGCAGGAGAAGCGGTGAT
GGAGAGCCGAGCTCGCCCCTTCCAAGCGCTGCCCCGTGAGCAGTCTCCAC
CACCTCCCCTGCAAACGTCCAGTGGTGCAGAGGTAATGGACGTTGGCTCT
GGTGGTGATGGACAGTCCGAACTCCCTGCTGAGGACCCCTTCAACTTCTA
CGGAGCTTCTCTTCTCTCCAAAGGATCCTTCTCTAAGGGCCGCCTCCTCA
TAGACCCGAACTGTAGTGGCCACAGCCCGCGCACCGCCCGGCACGCACCT
GCGGTCCGGAAGTTCTCCCCTGACCTTAAGTTGCTTAAGGATGTAAAGAT
TAGCGTGAGCTTTACCGAGAGCTGCAGGAGTAAGGACAGGAAGGTGCTGT
ACACAGGAGCAGAGCGCGACGTGCGGGCGGAGTGCGGTCTGCTCCTTAGC
CCTGTCAGTGGGGACGTGCATGCTTGTCCCTTTGGCGGGAGTGTTGGTGA
CGGGGTAGGCATAGGGGGTGAGAGTGCTGATAAGAAGGATGAGGAGAATG
AGCTGGATCAGGAAAAGAGAGTGGAGTATGCAGTGCTCGATGAGTTAGAA
GATTTTACTGACAATTTGGAGCTAGATGAAGAAGGAGCAGGCGGGTTCAC
GGCTAAAGCAATCGTTCAGAGAGACAGAGTGGATGAAGAGGCCCTGAATT
TCCCCTACGAGGATGACTTTGACAACGATGTGGATGCTCTGCTGGAAGAA
GGCCTTTGTGCCCCCAAAAAGAGGCGAACAGAGGAAAAATATGGCGGAGA
CAGCGACCATCCGTCCGATGGAGAGACAAGTGTGCAGCCGATGATGACCA
AGATTAAAACAGTGCTCAAAAGTCGTGGCCGCCCACCTACAGAGCCGCTG
CCCGACGGGTGGATCATGACATTCCATAACTCTGGAGTCCCGGTGTACCT
ACACAGAGAGTCTCGGGTGGTCACCTGGTCCAGGCCATACTTCTTGGGAA
CGGGAAGCATACGGAAACACGACCCTCCTCTGAGTAGCATCCCTTGTCTG
CATTATAAGAAAATGAAGGACAACGAGGAACGGGAGCAAAGCAGTGACCT
CACCCCTAGTGGGGATGTGTCCCCCGTCAAGCCCCTGAGCCGATCTGCAG
AGCTGGAGTTTCCCCTGGATGAGCCTGACTCTATGGGTGCTGACCCGGGG
CCCCCGGACGAGAAAGACCCACTAGGGGCTGAGGCAGCCCCTGGGGCCCT
GGGGCAGGTGAAGGCCAAAGTCGAGGTGTGCAAAGATGAATCCGTTGATC
TCGAGGAATTTCGAAGCTACCTGGAGAAGCGTTTTGACTTTGAGCAAGTT
ACTGTGAAAAAATTCAGGACTTGGGCTGAGCGGCGGCAATTCAATCGGGA
AATGAAGCGGAAGCAGGCGGAGTCCGAGAGGCCCATCTTGCCAGCCAATC
AGAAGCTCATTACTTTATCAGTGCAAGATGCACCCACAAAGAAAGAGTTT
GTTATTAACCCCAACGGGAAATCCGAGGTCTGCATCCTGCACGAGTACAT
GCAGCGTGTCCTCAAGGTCCGCCCTGTCTATAATTTCTTTGAATGTGAGA
ACCCAAGTGAGCCTTTTGGTGCCTCGGTGACCATTGATGGTGTGACTTAC
GGATCTGGAACTGCAAGCAGCAAAAAACTTGCGAAGAATAAAGCTGCCCG
AGCTACACTGGAAATCCTCATCCCTGACTTTGTTAAACAGACCTCTGAAG
AGAAGCCCAAAGACAGTGAAGAACTCGAGTATTTTAACCACATCAGCATC
GAGGACTCGCGGGTCTACGAGCTGACCAGCAAGGCTGGGCTGTTGTCTCC
ATATCAGATCCTCCACGAGTGCCTTAAAAGAAACCATGGGATGGGTGACA
CGTCTATCAAGTTTGAAGTGGTTCCTGGGAAAAACCAGAAGAGTGAATAC
GTCATGGCGTGTGGCAAGCACACAGTGCGCGGGTGGTGTAAGAACAAGAG
AGTTGGAAAGCAGTTAGCCTCACAGAAGATCCTTCAGCTGCTGCACCCAC
ATGTCAAGAACTGGGGGTCTTTACTGCGCATGTATGGCCGTGAGAGCAGC
AAGATGGTCAAGCAGGAGACATCGGACAAGAGTGTGATTGAGCTGCAGCA
GTATGCCAAGAAGAACAAGCCCAACCTGCACATCCTCAGCAAGCTCCAAG
AGGAGATGAAGAGGCTAGCTGAGGAAAGGGAGGAGACTCGAAAGAAGCCC
AAGATGTCCATTGTGGCGTCCGCCCAGCCTGGCGGTGAGCCCCTGTGCAC
CGTGGACGTGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSMET
DESPSPLPCGPAGEAVMESRARPFQALPREQSPPPPLQTSSGAEVMDVGS
GGDGQSELPAEDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAP
AVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGAERDVRAECGLLLS
PVSGDVHACPFGGSVGDGVGIGGESADKKDEENELDQEKRVEYAVLDELE
DFTDNLELDEEGAGGFTAKAIVQRDRVDEEALNFPYEDDFDNDVDALLEE
GLCAPKKRRTEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPL
PDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCL
HYKKMKDNEEREQSSDLTPSGDVSPVKPLSRSAELEFPLDEPDSMGADPG
PPDEKDPLGAEAAPGALGQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQV
TVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEF
VINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTY
GSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISI
EDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEY
VMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESS
KMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKP
KMSIVASAQPGGEPLCTVDV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4586
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GFP tag.