cDNA Clone DGCR8

• Plasmid: pEBG6P-1 DGCR8 FL
• Parent Plasmid: pEBG6P
• Expressed: GST-DGCR8 FL
• Genbank: CR456356
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCTATGGAGACAGATGAGAGCCCCTCTCCGCTCCCGTGTGGG
CCCGCAGGAGAAGCGGTGATGGAGAGCCGAGCTCGCCCCTTCCAAGCGCT
GCCCCGTGAGCAGTCTCCACCACCTCCCCTGCAAACGTCCAGTGGTGCAG
AGGTAATGGACGTTGGCTCTGGTGGTGATGGACAGTCCGAACTCCCTGCT
GAGGACCCCTTCAACTTCTACGGAGCTTCTCTTCTCTCCAAAGGATCCTT
CTCTAAGGGCCGCCTCCTCATAGACCCGAACTGTAGTGGCCACAGCCCGC
GCACCGCCCGGCACGCACCTGCGGTCCGGAAGTTCTCCCCTGACCTTAAG
TTGCTTAAGGATGTAAAGATTAGCGTGAGCTTTACCGAGAGCTGCAGGAG
TAAGGACAGGAAGGTGCTGTACACAGGAGCAGAGCGCGACGTGCGGGCGG
AGTGCGGTCTGCTCCTTAGCCCTGTCAGTGGGGACGTGCATGCTTGTCCC
TTTGGCGGGAGTGTTGGTGACGGGGTAGGCATAGGGGGTGAGAGTGCTGA
TAAGAAGGATGAGGAGAATGAGCTGGATCAGGAAAAGAGAGTGGAGTATG
CAGTGCTCGATGAGTTAGAAGATTTTACTGACAATTTGGAGCTAGATGAA
GAAGGAGCAGGCGGGTTCACGGCTAAAGCAATCGTTCAGAGAGACAGAGT
GGATGAAGAGGCCCTGAATTTCCCCTACGAGGATGACTTTGACAACGATG
TGGATGCTCTGCTGGAAGAAGGCCTTTGTGCCCCCAAAAAGAGGCGAACA
GAGGAAAAATATGGCGGAGACAGCGACCATCCGTCCGATGGAGAGACAAG
TGTGCAGCCGATGATGACCAAGATTAAAACAGTGCTCAAAAGTCGTGGCC
GCCCACCTACAGAGCCGCTGCCCGACGGGTGGATCATGACATTCCATAAC
TCTGGAGTCCCGGTGTACCTACACAGAGAGTCTCGGGTGGTCACCTGGTC
CAGGCCATACTTCTTGGGAACGGGAAGCATACGGAAACACGACCCTCCTC
TGAGTAGCATCCCTTGTCTGCATTATAAGAAAATGAAGGACAACGAGGAA
CGGGAGCAAAGCAGTGACCTCACCCCTAGTGGGGATGTGTCCCCCGTCAA
GCCCCTGAGCCGATCTGCAGAGCTGGAGTTTCCCCTGGATGAGCCTGACT
CTATGGGTGCTGACCCGGGGCCCCCGGACGAGAAAGACCCACTAGGGGCT
GAGGCAGCCCCTGGGGCCCTGGGGCAGGTGAAGGCCAAAGTCGAGGTGTG
CAAAGATGAATCCGTTGATCTCGAGGAATTTCGAAGCTACCTGGAGAAGC
GTTTTGACTTTGAGCAAGTTACTGTGAAAAAATTCAGGACTTGGGCTGAG
CGGCGGCAATTCAATCGGGAAATGAAGCGGAAGCAGGCGGAGTCCGAGAG
GCCCATCTTGCCAGCCAATCAGAAGCTCATTACTTTATCAGTGCAAGATG
CACCCACAAAGAAAGAGTTTGTTATTAACCCCAACGGGAAATCCGAGGTC
TGCATCCTGCACGAGTACATGCAGCGTGTCCTCAAGGTCCGCCCTGTCTA
TAATTTCTTTGAATGTGAGAACCCAAGTGAGCCTTTTGGTGCCTCGGTGA
CCATTGATGGTGTGACTTACGGATCTGGAACTGCAAGCAGCAAAAAACTT
GCGAAGAATAAAGCTGCCCGAGCTACACTGGAAATCCTCATCCCTGACTT
TGTTAAACAGACCTCTGAAGAGAAGCCCAAAGACAGTGAAGAACTCGAGT
ATTTTAACCACATCAGCATCGAGGACTCGCGGGTCTACGAGCTGACCAGC
AAGGCTGGGCTGTTGTCTCCATATCAGATCCTCCACGAGTGCCTTAAAAG
AAACCATGGGATGGGTGACACGTCTATCAAGTTTGAAGTGGTTCCTGGGA
AAAACCAGAAGAGTGAATACGTCATGGCGTGTGGCAAGCACACAGTGCGC
GGGTGGTGTAAGAACAAGAGAGTTGGAAAGCAGTTAGCCTCACAGAAGAT
CCTTCAGCTGCTGCACCCACATGTCAAGAACTGGGGGTCTTTACTGCGCA
TGTATGGCCGTGAGAGCAGCAAGATGGTCAAGCAGGAGACATCGGACAAG
AGTGTGATTGAGCTGCAGCAGTATGCCAAGAAGAACAAGCCCAACCTGCA
CATCCTCAGCAAGCTCCAAGAGGAGATGAAGAGGCTAGCTGAGGAAAGGG
AGGAGACTCGAAAGAAGCCCAAGATGTCCATTGTGGCGTCCGCCCAGCCT
GGCGGTGAGCCCCTGTGCACCGTGGACGTGTGAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMETDESPSPLPCG
PAGEAVMESRARPFQALPREQSPPPPLQTSSGAEVMDVGSGGDGQSELPA
EDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLK
LLKDVKISVSFTESCRSKDRKVLYTGAERDVRAECGLLLSPVSGDVHACP
FGGSVGDGVGIGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDE
EGAGGFTAKAIVQRDRVDEEALNFPYEDDFDNDVDALLEEGLCAPKKRRT
EEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHN
SGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEE
REQSSDLTPSGDVSPVKPLSRSAELEFPLDEPDSMGADPGPPDEKDPLGA
EAAPGALGQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTVKKFRTWAE
RRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEV
CILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKL
AKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTS
KAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVR
GWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDK
SVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKPKMSIVASAQP
GGEPLCTVDV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4575
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST tag.