cDNA Clone DGCR8

• Plasmid: pCMV5-FLAG-1 DGCR8 FL
• Parent Plasmid: pCMV5
• Expressed: FLAG-DGCR8 FL
• Genbank: CR456356
• Tag: FLAG
• Species: Human

Sequence of Insert

GCCACCATGGACTACAAGGACGATGACGATAAGGGATCTATGGAGACAGA
TGAGAGCCCCTCTCCGCTCCCGTGTGGGCCCGCAGGAGAAGCGGTGATGG
AGAGCCGAGCTCGCCCCTTCCAAGCGCTGCCCCGTGAGCAGTCTCCACCA
CCTCCCCTGCAAACGTCCAGTGGTGCAGAGGTAATGGACGTTGGCTCTGG
TGGTGATGGACAGTCCGAACTCCCTGCTGAGGACCCCTTCAACTTCTACG
GAGCTTCTCTTCTCTCCAAAGGATCCTTCTCTAAGGGCCGCCTCCTCATA
GACCCGAACTGTAGTGGCCACAGCCCGCGCACCGCCCGGCACGCACCTGC
GGTCCGGAAGTTCTCCCCTGACCTTAAGTTGCTTAAGGATGTAAAGATTA
GCGTGAGCTTTACCGAGAGCTGCAGGAGTAAGGACAGGAAGGTGCTGTAC
ACAGGAGCAGAGCGCGACGTGCGGGCGGAGTGCGGTCTGCTCCTTAGCCC
TGTCAGTGGGGACGTGCATGCTTGTCCCTTTGGCGGGAGTGTTGGTGACG
GGGTAGGCATAGGGGGTGAGAGTGCTGATAAGAAGGATGAGGAGAATGAG
CTGGATCAGGAAAAGAGAGTGGAGTATGCAGTGCTCGATGAGTTAGAAGA
TTTTACTGACAATTTGGAGCTAGATGAAGAAGGAGCAGGCGGGTTCACGG
CTAAAGCAATCGTTCAGAGAGACAGAGTGGATGAAGAGGCCCTGAATTTC
CCCTACGAGGATGACTTTGACAACGATGTGGATGCTCTGCTGGAAGAAGG
CCTTTGTGCCCCCAAAAAGAGGCGAACAGAGGAAAAATATGGCGGAGACA
GCGACCATCCGTCCGATGGAGAGACAAGTGTGCAGCCGATGATGACCAAG
ATTAAAACAGTGCTCAAAAGTCGTGGCCGCCCACCTACAGAGCCGCTGCC
CGACGGGTGGATCATGACATTCCATAACTCTGGAGTCCCGGTGTACCTAC
ACAGAGAGTCTCGGGTGGTCACCTGGTCCAGGCCATACTTCTTGGGAACG
GGAAGCATACGGAAACACGACCCTCCTCTGAGTAGCATCCCTTGTCTGCA
TTATAAGAAAATGAAGGACAACGAGGAACGGGAGCAAAGCAGTGACCTCA
CCCCTAGTGGGGATGTGTCCCCCGTCAAGCCCCTGAGCCGATCTGCAGAG
CTGGAGTTTCCCCTGGATGAGCCTGACTCTATGGGTGCTGACCCGGGGCC
CCCGGACGAGAAAGACCCACTAGGGGCTGAGGCAGCCCCTGGGGCCCTGG
GGCAGGTGAAGGCCAAAGTCGAGGTGTGCAAAGATGAATCCGTTGATCTC
GAGGAATTTCGAAGCTACCTGGAGAAGCGTTTTGACTTTGAGCAAGTTAC
TGTGAAAAAATTCAGGACTTGGGCTGAGCGGCGGCAATTCAATCGGGAAA
TGAAGCGGAAGCAGGCGGAGTCCGAGAGGCCCATCTTGCCAGCCAATCAG
AAGCTCATTACTTTATCAGTGCAAGATGCACCCACAAAGAAAGAGTTTGT
TATTAACCCCAACGGGAAATCCGAGGTCTGCATCCTGCACGAGTACATGC
AGCGTGTCCTCAAGGTCCGCCCTGTCTATAATTTCTTTGAATGTGAGAAC
CCAAGTGAGCCTTTTGGTGCCTCGGTGACCATTGATGGTGTGACTTACGG
ATCTGGAACTGCAAGCAGCAAAAAACTTGCGAAGAATAAAGCTGCCCGAG
CTACACTGGAAATCCTCATCCCTGACTTTGTTAAACAGACCTCTGAAGAG
AAGCCCAAAGACAGTGAAGAACTCGAGTATTTTAACCACATCAGCATCGA
GGACTCGCGGGTCTACGAGCTGACCAGCAAGGCTGGGCTGTTGTCTCCAT
ATCAGATCCTCCACGAGTGCCTTAAAAGAAACCATGGGATGGGTGACACG
TCTATCAAGTTTGAAGTGGTTCCTGGGAAAAACCAGAAGAGTGAATACGT
CATGGCGTGTGGCAAGCACACAGTGCGCGGGTGGTGTAAGAACAAGAGAG
TTGGAAAGCAGTTAGCCTCACAGAAGATCCTTCAGCTGCTGCACCCACAT
GTCAAGAACTGGGGGTCTTTACTGCGCATGTATGGCCGTGAGAGCAGCAA
GATGGTCAAGCAGGAGACATCGGACAAGAGTGTGATTGAGCTGCAGCAGT
ATGCCAAGAAGAACAAGCCCAACCTGCACATCCTCAGCAAGCTCCAAGAG
GAGATGAAGAGGCTAGCTGAGGAAAGGGAGGAGACTCGAAAGAAGCCCAA
GATGTCCATTGTGGCGTCCGCCCAGCCTGGCGGTGAGCCCCTGTGCACCG
TGGACGTGTGAGCGGCCGCG

Amino Acid Sequence

MDYKDDDDKGSMETDESPSPLPCGPAGEAVMESRARPFQALPREQSPPPP
LQTSSGAEVMDVGSGGDGQSELPAEDPFNFYGASLLSKGSFSKGRLLIDP
NCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTG
AERDVRAECGLLLSPVSGDVHACPFGGSVGDGVGIGGESADKKDEENELD
QEKRVEYAVLDELEDFTDNLELDEEGAGGFTAKAIVQRDRVDEEALNFPY
EDDFDNDVDALLEEGLCAPKKRRTEEKYGGDSDHPSDGETSVQPMMTKIK
TVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGS
IRKHDPPLSSIPCLHYKKMKDNEEREQSSDLTPSGDVSPVKPLSRSAELE
FPLDEPDSMGADPGPPDEKDPLGAEAAPGALGQVKAKVEVCKDESVDLEE
FRSYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKL
ITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPS
EPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKP
KDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSI
KFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVK
NWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEM
KRLAEEREETRKKPKMSIVASAQPGGEPLCTVDV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4574
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term FLAG tag.