cDNA Clone DGK theta

• Plasmid: pGEX6P-1-DGK theta
• Parent Plasmid: pGEX6P1
• Expressed: GST-DGK theta
• Genbank: NM_001347.2 GI:40806174*
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGGCGGCGGCGGCCGAGCCCGGGGCCCGCGCCTGGCTGGGCGG
CGGCTCCCCGCGCCCCGGCAGCCCGGCCTGCAGCCTCGTGCTGGGCTCAG
GAGGCCGCGCGCGCCCGGGGCCGGGGCCGGGGCCGGGACCCGAGCGGGCG
GGCGTCAGAGCCCCGGGCCCCGCTGCCGCGCCGGGACACAGCTTCCGGAA
GGTGACGCTCACCAAGCCCACCTTCTGCCACCTCTGCTCCGACTTCATCT
GGGGGCTGGCCGGCTTCCTGTGCGACGTCTGCAATTTCATGTCTCATGAG
AAGTGCCTGAAGCACGTGAGGATCCCGTGCACGAGTGTGGCACCCAGCCT
GGTCCGGGTTCCTGTAGCCCACTGCTTCGGCCCCCGGGGGCTCCACAAGC
GCAAGTTCTGTGCTGTCTGCCGCAAGGTCCTGGAGGCACCGGCGCTCCAC
TGCGAAGTGTGTGAGCTGCACCTCCACCCAGACTGTGTGCCCTTCGCCTG
CAGTGACTGCCGCCAGTGCCACCAGGATGGGCACCAGGATCACGACACCC
ATCACCACCACTGGCGGGAGGGGAACCTGCCCTCGGGAGCGCGCTGCGAG
GTCTGCAGGAAGACGTGCGGCTCCTCTGACGTGCTGGCCGGCGTGCGCTG
CGAGTGGTGCGGGGTCCAGGCGCACTCCCTCTGCTCCGCGGCACTGGCTC
CCGAGTGTGGCTTCGGGCGTCTGCGCTCCCTGGTCCTGCCTCCCGCGTGC
GTGCGCCTTCTGCCCGGCGGCTTCAGCAAGACGCAGAGCTTCCGCATCGT
GGAGGCCGCGGAGCCGGGCGAGGGGGGCGACGGCGCCGACGGGAGCGCTG
CCGTGGGTCCAGGCAGAGAGACACAGGCAACTCCGGAGTCCGGGAAGCAA
ACGCTGAAGATCTTTGATGGCGACGACGCGGTGAGAAGAAGCCAGTTCCG
CCTCGTCACGGTGTCCCGCCTGGCCGGTGCCGAGGAGGTGCTGGAGGCCG
CACTGCGGGCCCACCACATCCCCGAGGACCCTGGCCACCTGGAGCTGTGC
CGGCTGCCCCCTTCCTCTCAGGCCTGTGACGCCTGGGCTGGGGGCAAGGC
TGGGAGTGCTGTGATCTCGGAGGAGGGCAGAAGCCCCGGGTCCGGCGAGG
CCACGCCAGAGGCCTGGGTCATCCGGGCTCTGCCGCGGGCCCAGGAGGTC
CTGAAGATCTACCCTGGCTGGCTCAAGGTGGGCGTGGCCTACGTGTCCGT
GCGAGTGACCCCGAAGAGCACGGCCCGCTCTGTGGTGCTGGAGGTCCTGC
CGCTGCTCGGCCGCCAGGCCGAGAGTCCCGAGAGCTTCCAGCTGGTGGAG
GTGGCGATGGGCTGCAGGCACGTCCAGCGGACGATGCTGATGGACGAACA
GCCCCTGCTGGACCGGCTACAGGACATCCGGCAGATGTCTGTGCGGCAGG
TGAGCCAGACGCGGTTCTACGTGGCAGAGAGCAGGGATGTAGCCCCGCAC
GTCTCCCTGTTTGTTGGCGGCCTGCCTCCCGGCCTGTCTCCCGAGGAGTA
CAGCAGCCTGCTGCATGAGGCCGGGGCTACCAAAGCCACCGTGGTGTCCG
TGAGTCACATCTACTCCTCCCAAGGCGCGGTAGTGTTGGACGTTGCCTGC
TTTGCGGAGGCCGAGCGGCTGTACATGCTGCTGAAGGACATGGCTGTGCG
GGGCCGGCTGCTCACTGCCCTGGTGCTCCCCGACCTGCTGCACGCGAAGC
TGCCCCCAGACAGCTGTCCCCTCCTTGTGTTCGTGAACCCCAAGAGTGGA
GGCCTCAAGGGCCGAGACCTGCTCTGCAGCTTCCGGAAGCTACTGAACCC
TCATCAGGTCTTCGACCTGACCAACGGAGGTCCTCTTCCCGGGCTCCACC
TGTTCTCCCAGGTGCCCTGCTTCCGGGTGCTGGTGTGTGGTGGCGATGGC
ACTGTGGGCTGGGTGCTTGGCGCCCTGGAGGAGACACGGTACCGACTGGC
CTGCCCGGAGCCTTCTGTGGCCATCCTGCCCCTGGGCACAGGGAATGACC
TTGGTCGAGTCCTCCGCTGGGGGGCGGGCTACAGCGGCGAGGACCCGTTC
TCCGTACTGCTGTCTGTGGACGAGGCCGACGCCGTGCTCATGGACCGCTG
GACCATCCTGCTGGATGCCCACGAGGCTGGCAGTGCAGAGAACGACACGG
CAGACGCAGAGCCCCCCAAGATCGTGCAGATGAGTAACTACTGTGGCATT
GGCATCGACGCGGAGCTGAGCCTGGACTTCCACCAGGCACGGGAAGAGGA
GCCTGGCAAGTTCACAAGCAGGCTGCACAACAAGGGTGTGTACGTGCGGG
TGGGGCTGCAGAAGATCAGTCACTCTCGGAGCCTGCACAAGCAGATCCGG
CTGCAGGTGGAGCGGCAGGAGGTGGAGCTGCCCAGTATTGAAGGCCTCAT
CTTCATCAACATCCCCAGCTGGGGCTCGGGGGCCGACCTGTGGGGCTCCG
ACAGCGACACCAGGTTTGAGAAGCCACGCATGGACGACGGGCTGCTGGAG
GTTGTGGGCGTGACGGGCGTCGTGCACATGGGCCAGGTCCAGGGTGGGCT
GCGCTCCGGAATCCGGATTGCCCAGGGTTCCTACTTCCGAGTCACGCTCC
TCAAGGCCACCCCGGTGCAGGTGGACGGGGAGCCCTGGGTCCAGGCCCCG
GGGCACATGATCATCTCAGCTGCTGGCCCTAAGGTGCACATGCTGAGGAA
GGCCAAGCAGAAGCCGAGGAGGGCCGGGACCACCAGGGATGCCCGGGCGG
ATGCTGCGCCTGCCCCTGAGAGCGATCCTAGGTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMAAAAEPGARAWLGGG
SPRPGSPACSLVLGSGGRARPGPGPGPGPERAGVRAPGPAAAPGHSFRKV
TLTKPTFCHLCSDFIWGLAGFLCDVCNFMSHEKCLKHVRIPCTSVAPSLV
RVPVAHCFGPRGLHKRKFCAVCRKVLEAPALHCEVCELHLHPDCVPFACS
DCRQCHQDGHQDHDTHHHHWREGNLPSGARCEVCRKTCGSSDVLAGVRCE
WCGVQAHSLCSAALAPECGFGRLRSLVLPPACVRLLPGGFSKTQSFRIVE
AAEPGEGGDGADGSAAVGPGRETQATPESGKQTLKIFDGDDAVRRSQFRL
VTVSRLAGAEEVLEAALRAHHIPEDPGHLELCRLPPSSQACDAWAGGKAG
SAVISEEGRSPGSGEATPEAWVIRALPRAQEVLKIYPGWLKVGVAYVSVR
VTPKSTARSVVLEVLPLLGRQAESPESFQLVEVAMGCRHVQRTMLMDEQP
LLDRLQDIRQMSVRQVSQTRFYVAESRDVAPHVSLFVGGLPPGLSPEEYS
SLLHEAGATKATVVSVSHIYSSQGAVVLDVACFAEAERLYMLLKDMAVRG
RLLTALVLPDLLHAKLPPDSCPLLVFVNPKSGGLKGRDLLCSFRKLLNPH
QVFDLTNGGPLPGLHLFSQVPCFRVLVCGGDGTVGWVLGALEETRYRLAC
PEPSVAILPLGTGNDLGRVLRWGAGYSGEDPFSVLLSVDEADAVLMDRWT
ILLDAHEAGSAENDTADAEPPKIVQMSNYCGIGIDAELSLDFHQAREEEP
GKFTSRLHNKGVYVRVGLQKISHSRSLHKQIRLQVERQEVELPSIEGLIF
INIPSWGSGADLWGSDSDTRFEKPRMDDGLLEVVGVTGVVHMGQVQGGLR
SGIRIAQGSYFRVTLLKATPVQVDGEPWVQAPGHMIISAAGPKVHMLRKA
KQKPRRAGTTRDARADAAPAPESDPR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36166
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence differences compared with NM_001347.2 GI:40806174 CDS:; c80t (P27L); g687a (silent); -matches EST sequence BC063801.1 GI:39645109; Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 128363 Daltons