cDNA Clone DGK theta

• Plasmid: pFBHTa DGK theta
• Parent Plasmid: pSC-A
• Expressed: 6His DGK theta
• Genbank: NM_001347.2 GI:40806174*
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCATGGCGGCGGCGGCCGAGCCCGGGGCCCGCGCCTGGCTGGGCGG
CGGCTCCCCGCGCCCCGGCAGCCCGGCCTGCAGCCTCGTGCTGGGCTCAG
GAGGCCGCGCGCGCCCGGGGCCGGGGCCGGGGCCGGGACCCGAGCGGGCG
GGCGTCAGAGCCCCGGGCCCCGCTGCCGCGCCGGGACACAGCTTCCGGAA
GGTGACGCTCACCAAGCCCACCTTCTGCCACCTCTGCTCCGACTTCATCT
GGGGGCTGGCCGGCTTCCTGTGCGACGTCTGCAATTTCATGTCTCATGAG
AAGTGCCTGAAGCACGTGAGGATCCCGTGCACGAGTGTGGCACCCAGCCT
GGTCCGGGTTCCTGTAGCCCACTGCTTCGGCCCCCGGGGGCTCCACAAGC
GCAAGTTCTGTGCTGTCTGCCGCAAGGTCCTGGAGGCACCGGCGCTCCAC
TGCGAAGTGTGTGAGCTGCACCTCCACCCAGACTGTGTGCCCTTCGCCTG
CAGTGACTGCCGCCAGTGCCACCAGGATGGGCACCAGGATCACGACACCC
ATCACCACCACTGGCGGGAGGGGAACCTGCCCTCGGGAGCGCGCTGCGAG
GTCTGCAGGAAGACGTGCGGCTCCTCTGACGTGCTGGCCGGCGTGCGCTG
CGAGTGGTGCGGGGTCCAGGCGCACTCCCTCTGCTCCGCGGCACTGGCTC
CCGAGTGTGGCTTCGGGCGTCTGCGCTCCCTGGTCCTGCCTCCCGCGTGC
GTGCGCCTTCTGCCCGGCGGCTTCAGCAAGACGCAGAGCTTCCGCATCGT
GGAGGCCGCGGAGCCGGGCGAGGGGGGCGACGGCGCCGACGGGAGCGCTG
CCGTGGGTCCAGGCAGAGAGACACAGGCAACTCCGGAGTCCGGGAAGCAA
ACGCTGAAGATCTTTGATGGCGACGACGCGGTGAGAAGAAGCCAGTTCCG
CCTCGTCACGGTGTCCCGCCTGGCCGGTGCCGAGGAGGTGCTGGAGGCCG
CACTGCGGGCCCACCACATCCCCGAGGACCCTGGCCACCTGGAGCTGTGC
CGGCTGCCCCCTTCCTCTCAGGCCTGTGACGCCTGGGCTGGGGGCAAGGC
TGGGAGTGCTGTGATCTCGGAGGAGGGCAGAAGCCCCGGGTCCGGCGAGG
CCACGCCAGAGGCCTGGGTCATCCGGGCTCTGCCGCGGGCCCAGGAGGTC
CTGAAGATCTACCCTGGCTGGCTCAAGGTGGGCGTGGCCTACGTGTCCGT
GCGAGTGACCCCGAAGAGCACGGCCCGCTCTGTGGTGCTGGAGGTCCTGC
CGCTGCTCGGCCGCCAGGCCGAGAGTCCCGAGAGCTTCCAGCTGGTGGAG
GTGGCGATGGGCTGCAGGCACGTCCAGCGGACGATGCTGATGGACGAACA
GCCCCTGCTGGACCGGCTACAGGACATCCGGCAGATGTCTGTGCGGCAGG
TGAGCCAGACGCGGTTCTACGTGGCAGAGAGCAGGGATGTAGCCCCGCAC
GTCTCCCTGTTTGTTGGCGGCCTGCCTCCCGGCCTGTCTCCCGAGGAGTA
CAGCAGCCTGCTGCATGAGGCCGGGGCTACCAAAGCCACCGTGGTGTCCG
TGAGTCACATCTACTCCTCCCAAGGCGCGGTAGTGTTGGACGTTGCCTGC
TTTGCGGAGGCCGAGCGGCTGTACATGCTGCTGAAGGACATGGCTGTGCG
GGGCCGGCTGCTCACTGCCCTGGTGCTCCCCGACCTGCTGCACGCGAAGC
TGCCCCCAGACAGCTGTCCCCTCCTTGTGTTCGTGAACCCCAAGAGTGGA
GGCCTCAAGGGCCGAGACCTGCTCTGCAGCTTCCGGAAGCTACTGAACCC
TCATCAGGTCTTCGACCTGACCAACGGAGGTCCTCTTCCCGGGCTCCACC
TGTTCTCCCAGGTGCCCTGCTTCCGGGTGCTGGTGTGTGGTGGCGATGGC
ACTGTGGGCTGGGTGCTTGGCGCCCTGGAGGAGACACGGTACCGACTGGC
CTGCCCGGAGCCTTCTGTGGCCATCCTGCCCCTGGGCACAGGGAATGACC
TTGGTCGAGTCCTCCGCTGGGGGGCGGGCTACAGCGGCGAGGACCCGTTC
TCCGTACTGCTGTCTGTGGACGAGGCCGACGCCGTGCTCATGGACCGCTG
GACCATCCTGCTGGATGCCCACGAGGCTGGCAGTGCAGAGAACGACACGG
CAGACGCAGAGCCCCCCAAGATCGTGCAGATGAGTAACTACTGTGGCATT
GGCATCGACGCGGAGCTGAGCCTGGACTTCCACCAGGCACGGGAAGAGGA
GCCTGGCAAGTTCACAAGCAGGCTGCACAACAAGGGTGTGTACGTGCGGG
TGGGGCTGCAGAAGATCAGTCACTCTCGGAGCCTGCACAAGCAGATCCGG
CTGCAGGTGGAGCGGCAGGAGGTGGAGCTGCCCAGTATTGAAGGCCTCAT
CTTCATCAACATCCCCAGCTGGGGCTCGGGGGCCGACCTGTGGGGCTCCG
ACAGCGACACCAGGTTTGAGAAGCCACGCATGGACGACGGGCTGCTGGAG
GTTGTGGGCGTGACGGGCGTCGTGCACATGGGCCAGGTCCAGGGTGGGCT
GCGCTCCGGAATCCGGATTGCCCAGGGTTCCTACTTCCGAGTCACGCTCC
TCAAGGCCACCCCGGTGCAGGTGGACGGGGAGCCCTGGGTCCAGGCCCCG
GGGCACATGATCATCTCAGCTGCTGGCCCTAAGGTGCACATGCTGAGGAA
GGCCAAGCAGAAGCCGAGGAGGGCCGGGACCACCAGGGATGCCCGGGCGG
ATGCTGCGCCTGCCCCTGAGAGCGATCCTAGGTAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMAAAAEPGARAWLGGGSPRP
GSPACSLVLGSGGRARPGPGPGPGPERAGVRAPGPAAAPGHSFRKVTLTK
PTFCHLCSDFIWGLAGFLCDVCNFMSHEKCLKHVRIPCTSVAPSLVRVPV
AHCFGPRGLHKRKFCAVCRKVLEAPALHCEVCELHLHPDCVPFACSDCRQ
CHQDGHQDHDTHHHHWREGNLPSGARCEVCRKTCGSSDVLAGVRCEWCGV
QAHSLCSAALAPECGFGRLRSLVLPPACVRLLPGGFSKTQSFRIVEAAEP
GEGGDGADGSAAVGPGRETQATPESGKQTLKIFDGDDAVRRSQFRLVTVS
RLAGAEEVLEAALRAHHIPEDPGHLELCRLPPSSQACDAWAGGKAGSAVI
SEEGRSPGSGEATPEAWVIRALPRAQEVLKIYPGWLKVGVAYVSVRVTPK
STARSVVLEVLPLLGRQAESPESFQLVEVAMGCRHVQRTMLMDEQPLLDR
LQDIRQMSVRQVSQTRFYVAESRDVAPHVSLFVGGLPPGLSPEEYSSLLH
EAGATKATVVSVSHIYSSQGAVVLDVACFAEAERLYMLLKDMAVRGRLLT
ALVLPDLLHAKLPPDSCPLLVFVNPKSGGLKGRDLLCSFRKLLNPHQVFD
LTNGGPLPGLHLFSQVPCFRVLVCGGDGTVGWVLGALEETRYRLACPEPS
VAILPLGTGNDLGRVLRWGAGYSGEDPFSVLLSVDEADAVLMDRWTILLD
AHEAGSAENDTADAEPPKIVQMSNYCGIGIDAELSLDFHQAREEEPGKFT
SRLHNKGVYVRVGLQKISHSRSLHKQIRLQVERQEVELPSIEGLIFINIP
SWGSGADLWGSDSDTRFEKPRMDDGLLEVVGVTGVVHMGQVQGGLRSGIR
IAQGSYFRVTLLKATPVQVDGEPWVQAPGHMIISAAGPKVHMLRKAKQKP
RRAGTTRDARADAAPAPESDPR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU31008
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence differences compared with NM_001347.2 GI:40806174 CDS:; c80t (P27L); g687a (silent); -matches EST sequence BC063801.1 GI:39645109; ; Baculovirus expression plasmid with N terminal 6His tag; ; Estimated MW of expressed protein : 104881 Daltons