cDNA Clone DISC1

• Plasmid: pGEX6P-1-DISC1
• Parent Plasmid: pGEX6P1
• Expressed: GST-DISC1
• Genbank: AF222980.1 GI:8163868
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGCCAGGCGGGGGTCCTCAGGGCGCCCCAGCCGCCGCCGGCGGCGGCGGCGTGAGCCACCGCGCAGGCAGCCGGGATTGCTTACCACCTGCAGCGTGCTTTCGGAGGCGGCGGCTGGCACGGAGGCCGGGCTACATGAGAAGCTCGACAGGGCCTGGGATCGGGTTCCTTTCCCCAGCAGTGGGCACACTGTTCCGGTTCCCAGGAGGGGTGTCTGGCGAGGAGTCCCACCACTCGGAGTCCAGGGCCAGACAGTGTGGCCTTGACTCGAGAGGCCTCTTGGTCCGGAGCCCTGTTTCC
AAGAGTGCAGCAGCCCCTACTGTGACCTCTGTGAGAGGAACCTCGGCGCA
CTTTGGGATTCAGCTCAGAGGTGGCACCAGATTGCCTGACAGGCTTAGCT
GGCCGTGTGGCCCTGGGAGTGCTGGGTGGCAGCAAGAGTTTGCAGCCATG
GATAGTTCTGAGACCCTGGACGCCAGCTGGGAGGCAGCCTGCAGCGATGG
AGCAAGGCGTGTCCGGGCAGCAGGCTCTCTGCCATCAGCAGAGTTGAGTA
GCAACAGCTGCAGCCCTGGCTGTGGCCCTGAGGTCCCCCCAACCCCTCCT
GGCTCTCACAGTGCCTTTACCTCAAGCTTTAGCTTTATTCGGCTCTCGCT
TGGCTCTGCCGGGGAACGTGGAGAAGCAGAAGGCTGCCCACCATCCAGAG
AGGCTGAGTCCCATTGCCAGAGCCCCCAGGAGATGGGAGCCAAAGCTGCC
AGCTTGGACGGGCCTCACGAGGACCCGCGATGTCTCTCTCAGCCCTTCAG
TCTCTTGGCTACACGGGTCTCTGCAGACTTGGCCCAGGCCGCAAGGAACA
GCTCCAGGCCAGAGCGTGACATGCATTCTTTACCAGACATGGACCCTGGC
TCCTCCAGTTCTCTGGATCCCTCACTGGCTGGCTGTGGTGGTGATGGGAG
CAGCGGCTCAGGGGATGCCCACTCTTGGGACACCCTGCTCAGGAAATGGG
AGCCAGTGCTGCGGGACTGCCTGCTGAGAAACCGGAGGCAGATGGAGGTA
ATATCCTTAAGATTAAAACTTCAGAAACTTCAGGAAGATGCAGTTGAGAA
TGATGATTATGATAAAGCTGAGACGTTACAACAAAGATTAGAAGACCTGG
AACAAGAGAAAATCAGCCTGCACTTTCAACTTCCTTCAAGGCAGCCAGCT
CTTAGCAGTTTCCTGGGTCACCTGGCAGCACAAGTCCAGGCTGCCTTGCG
CCGTGGGGCCACTCAGCAGGCCAGCGGAGATGACACCCACACCCCACTGA
GAATGGAGCCGAGGCTGTTGGAACCCACTGCTCAGGACAGCTTGCACGTG
TCCATCACGAGACGAGACTGGCTTCTTCAGGAAAAGCAGCAGCTACAGAA
AGAAATCGAAGCTCTCCAAGCAAGGATGTTTGTGCTGGAAGCCAAAGATC
AACAGCTGAGAAGGGAAATAGAGGAGCAAGAGCAGCAACTCCAGTGGCAG
GGCTGCGACCTGACCCCACTGGTGGGCCAGCTGTCCCTGGGTCAGCTGCA
GGAGGTCAGCAAGGCCTTGCAGGACACCCTGGCCTCAGCCGGTCAGATTC
CCTTCCATGCAGAGCCACCGGAAACCATAAGGAGCCTCCAGGAAAGAATA
AAATCCCTCAACTTGTCACTTAAAGAAATCACTACTAAGGTGTGTATGAG
TGAGAAATTCTGCAGCACCCTGAGGAAGAAAGTTAACGATATTGAAACCC
AACTACCAGCCTTGCTTGAAGCCAAAATGCATGCCATATCAGGAAACCAT
TTCTGGACGGCTAAAGACCTCACCGAGGAGATTAGATCATTAACATCAGA
GAGAGAAGGGCTGGAGGGACTCCTCAGCAAGCTGTTGGTGTTGAGTTCCA
GGAATGTCAAAAAGCTGGGAAGTGTTAAAGAAGATTACAACAGACTGAGA
AGAGAAGTGGAGCACCAGGAGACTGCCTATGAAACAAGTGTGAAGGAAAA
TACTATGAAGTACATGGAAACACTTAAGAATAAACTGTGCAGCTGCAAGT
GTCCACTGCTTGGGAAAGTGTGGGAAGCTGACTTGGAAGCTTGTCGATTG
CTTATCCAGTGCCTACAGCTCCAGGAAGCCAGGGGAAGCCTGTCTGTAGA
AGATGAGAGGCAGATGGATGACTTAGAGGGAGCTGCTCCTCCTATTCCCC
CCAGGCTCCACTCCGAGGATAAAAGGAAGACCCCTTTGAAGGTATTGGAA
GAATGGAAGACTCACCTCATCCCCTCTCTGCACTGTGCTGGAGGTGAACA
GAAAGAGGAATCTTACATCCTTTCTGCAGAACTTGGAGAAAAGTGTGAAG
ACATAGGCAAGAAGCTATTGTACTTGGAAGATCAACTTCACACAGCAATC
CACAGTCATGATGAAGATCTCATTCAGTCTCTCAGGAGGGAGCTCCAGAT
GGTGAAGGAAACTCTGCAGGCCATGATCCTGCAGCTCCAGCCAGCAAAGG
AGGCGGGAGAAAGAGAAGCTGCAGCTTCCTGCATGACAGCTGGTGTCCAC
GAAGCACAAGCCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMPGGGPQGAPAAAGGG
GVSHRAGSRDCLPPAACFRRRRLARRPGYMRSSTGPGIGFLSPAVGTLFR
FPGGVSGEESHHSESRARQCGLDSRGLLVRSPVSKSAAAPTVTSVRGTSA
HFGIQLRGGTRLPDRLSWPCGPGSAGWQQEFAAMDSSETLDASWEAACSD
GARRVRAAGSLPSAELSSNSCSPGCGPEVPPTPPGSHSAFTSSFSFIRLS
LGSAGERGEAEGCPPSREAESHCQSPQEMGAKAASLDGPHEDPRCLSQPF
SLLATRVSADLAQAARNSSRPERDMHSLPDMDPGSSSSLDPSLAGCGGDG
SSGSGDAHSWDTLLRKWEPVLRDCLLRNRRQMEVISLRLKLQKLQEDAVE
NDDYDKAETLQQRLEDLEQEKISLHFQLPSRQPALSSFLGHLAAQVQAAL
RRGATQQASGDDTHTPLRMEPRLLEPTAQDSLHVSITRRDWLLQEKQQLQ
KEIEALQARMFVLEAKDQQLRREIEEQEQQLQWQGCDLTPLVGQLSLGQL
QEVSKALQDTLASAGQIPFHAEPPETIRSLQERIKSLNLSLKEITTKVCM
SEKFCSTLRKKVNDIETQLPALLEAKMHAISGNHFWTAKDLTEEIRSLTS
EREGLEGLLSKLLVLSSRNVKKLGSVKEDYNRLRREVEHQETAYETSVKE
NTMKYMETLKNKLCSCKCPLLGKVWEADLEACRLLIQCLQLQEARGSLSV
EDERQMDDLEGAAPPIPPRLHSEDKRKTPLKVLEEWKTHLIPSLHCAGGE
QKEESYILSAELGEKCEDIGKKLLYLEDQLHTAIHSHDEDLIQSLRRELQ
MVKETLQAMILQLQPAKEAGEREAAASCMTAGVHEAQA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU16929
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 120791 Daltons