cDNA Clone DISC1

• Plasmid: pDsRed-monomerC1 DISC1
• Parent Plasmid: pCMV DsRed Express
• Expressed: RFP-DISC1
• Genbank: AF222980.1 GI:8163868
• Species: Human

Sequence of Insert

GAATTCTATGCCAGGCGGGGGTCCTCAGGGCGCCCCAGCCGCCGCCGGCGGCGGCGGCGTGAGCCACCGCGCAGGCAGCCGGGATTGCTTACCACCTGCAGCGTGCTTTCGGAGGCGGCGGCTGGCACGGAGGCCGGGCTACATGAGAAGCTCGACAGGGCCTGGGATCGGGTTCCTTTCCCCAGCAGTGGGCACACTGTTCCGGTTCCCAGGAGGGGTGTCTGGCGAGGAGTCCCACCACTCGGAGTCCAGGGCCAGACAGTGTGGCCTTGACTCGAGAGGCCTCTTGGTCCGGAGCCCTGTTTCCAAGAGTGCAGCAGCCCCTACTGTGACCTCTGTGAGAGGAACCTCGGCGCA
CTTTGGGATTCAGCTCAGAGGTGGCACCAGATTGCCTGACAGGCTTAGCT
GGCCGTGTGGCCCTGGGAGTGCTGGGTGGCAGCAAGAGTTTGCAGCCATG
GATAGTTCTGAGACCCTGGACGCCAGCTGGGAGGCAGCCTGCAGCGATGG
AGCAAGGCGTGTCCGGGCAGCAGGCTCTCTGCCATCAGCAGAGTTGAGTA
GCAACAGCTGCAGCCCTGGCTGTGGCCCTGAGGTCCCCCCAACCCCTCCT
GGCTCTCACAGTGCCTTTACCTCAAGCTTTAGCTTTATTCGGCTCTCGCT
TGGCTCTGCCGGGGAACGTGGAGAAGCAGAAGGCTGCCCACCATCCAGAG
AGGCTGAGTCCCATTGCCAGAGCCCCCAGGAGATGGGAGCCAAAGCTGCC
AGCTTGGACGGGCCTCACGAGGACCCGCGATGTCTCTCTCAGCCCTTCAG
TCTCTTGGCTACACGGGTCTCTGCAGACTTGGCCCAGGCCGCAAGGAACA
GCTCCAGGCCAGAGCGTGACATGCATTCTTTACCAGACATGGACCCTGGC
TCCTCCAGTTCTCTGGATCCCTCACTGGCTGGCTGTGGTGGTGATGGGAG
CAGCGGCTCAGGGGATGCCCACTCTTGGGACACCCTGCTCAGGAAATGGG
AGCCAGTGCTGCGGGACTGCCTGCTGAGAAACCGGAGGCAGATGGAGGTA
ATATCCTTAAGATTAAAACTTCAGAAACTTCAGGAAGATGCAGTTGAGAA
TGATGATTATGATAAAGCTGAGACGTTACAACAAAGATTAGAAGACCTGG
AACAAGAGAAAATCAGCCTGCACTTTCAACTTCCTTCAAGGCAGCCAGCT
CTTAGCAGTTTCCTGGGTCACCTGGCAGCACAAGTCCAGGCTGCCTTGCG
CCGTGGGGCCACTCAGCAGGCCAGCGGAGATGACACCCACACCCCACTGA
GAATGGAGCCGAGGCTGTTGGAACCCACTGCTCAGGACAGCTTGCACGTG
TCCATCACGAGACGAGACTGGCTTCTTCAGGAAAAGCAGCAGCTACAGAA
AGAAATCGAAGCTCTCCAAGCAAGGATGTTTGTGCTGGAAGCCAAAGATC
AACAGCTGAGAAGGGAAATAGAGGAGCAAGAGCAGCAACTCCAGTGGCAG
GGCTGCGACCTGACCCCACTGGTGGGCCAGCTGTCCCTGGGTCAGCTGCA
GGAGGTCAGCAAGGCCTTGCAGGACACCCTGGCCTCAGCCGGTCAGATTC
CCTTCCATGCAGAGCCACCGGAAACCATAAGGAGCCTCCAGGAAAGAATA
AAATCCCTCAACTTGTCACTTAAAGAAATCACTACTAAGGTGTGTATGAG
TGAGAAATTCTGCAGCACCCTGAGGAAGAAAGTTAACGATATTGAAACCC
AACTACCAGCCTTGCTTGAAGCCAAAATGCATGCCATATCAGGAAACCAT
TTCTGGACGGCTAAAGACCTCACCGAGGAGATTAGATCATTAACATCAGA
GAGAGAAGGGCTGGAGGGACTCCTCAGCAAGCTGTTGGTGTTGAGTTCCA
GGAATGTCAAAAAGCTGGGAAGTGTTAAAGAAGATTACAACAGACTGAGA
AGAGAAGTGGAGCACCAGGAGACTGCCTATGAAACAAGTGTGAAGGAAAA
TACTATGAAGTACATGGAAACACTTAAGAATAAACTGTGCAGCTGCAAGT
GTCCACTGCTTGGGAAAGTGTGGGAAGCTGACTTGGAAGCTTGTCGATTG
CTTATCCAGTGCCTACAGCTCCAGGAAGCCAGGGGAAGCCTGTCTGTAGA
AGATGAGAGGCAGATGGATGACTTAGAGGGAGCTGCTCCTCCTATTCCCC
CCAGGCTCCACTCCGAGGATAAAAGGAAGACCCCTTTGAAGGTATTGGAA
GAATGGAAGACTCACCTCATCCCCTCTCTGCACTGTGCTGGAGGTGAACA
GAAAGAGGAATCTTACATCCTTTCTGCAGAACTTGGAGAAAAGTGTGAAG
ACATAGGCAAGAAGCTATTGTACTTGGAAGATCAACTTCACACAGCAATC
CACAGTCATGATGAAGATCTCATTCAGTCTCTCAGGAGGGAGCTCCAGAT
GGTGAAGGAAACTCTGCAGGCCATGATCCTGCAGCTCCAGCCAGCAAAGG
AGGCGGGAGAAAGAGAAGCTGCAGCTTCCTGCATGACAGCTGGTGTCCAC
GAAGCACAAGCCTGAGGTACC

Amino Acid Sequence

MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAKLQVTK
GGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFE
DGGVVEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKL
YPQDGVLKGEISHALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLD
ITNHNEDYTVVEQYEHAEARHSGSQSGLRSRAQASNSMPGGGPQGAPAAA
GGGGVSHRAGSRDCLPPAACFRRRRLARRPGYMRSSTGPGIGFLSPAVGT
LFRFPGGVSGEESHHSESRARQCGLDSRGLLVRSPVSKSAAAPTVTSVRG
TSAHFGIQLRGGTRLPDRLSWPCGPGSAGWQQEFAAMDSSETLDASWEAA
CSDGARRVRAAGSLPSAELSSNSCSPGCGPEVPPTPPGSHSAFTSSFSFI
RLSLGSAGERGEAEGCPPSREAESHCQSPQEMGAKAASLDGPHEDPRCLS
QPFSLLATRVSADLAQAARNSSRPERDMHSLPDMDPGSSSSLDPSLAGCG
GDGSSGSGDAHSWDTLLRKWEPVLRDCLLRNRRQMEVISLRLKLQKLQED
AVENDDYDKAETLQQRLEDLEQEKISLHFQLPSRQPALSSFLGHLAAQVQ
AALRRGATQQASGDDTHTPLRMEPRLLEPTAQDSLHVSITRRDWLLQEKQ
QLQKEIEALQARMFVLEAKDQQLRREIEEQEQQLQWQGCDLTPLVGQLSL
GQLQEVSKALQDTLASAGQIPFHAEPPETIRSLQERIKSLNLSLKEITTK
VCMSEKFCSTLRKKVNDIETQLPALLEAKMHAISGNHFWTAKDLTEEIRS
LTSEREGLEGLLSKLLVLSSRNVKKLGSVKEDYNRLRREVEHQETAYETS
VKENTMKYMETLKNKLCSCKCPLLGKVWEADLEACRLLIQCLQLQEARGS
LSVEDERQMDDLEGAAPPIPPRLHSEDKRKTPLKVLEEWKTHLIPSLHCA
GGEQKEESYILSAELGEKCEDIGKKLLYLEDQLHTAIHSHDEDLIQSLRR
ELQMVKETLQAMILQLQPAKEAGEREAAASCMTAGVHEAQA*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU16477
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression vector for expression of human DISC1 with N-terminal DsRed-Monomer.