cDNA Clone DGKA

• Synonyms: diacylglycerol kinase, alpha 80kDa1 2, DAG kinase alpha2 3 DGK-alpha1 2 3, EC 2.7.1.1073 8 DAGK12 3 5, diacylglycerol kinase, alpha (80kD)1 Diglyceride kinase alpha2 3, MGC128212 80 kDa diacylglycerol kinase2 3, MGC423562 DAGK2 3, diacylglycerol kinase alpha2
• Plasmid: pGEX6P-2-DGKA
• Parent Plasmid: pGEX6P2
• Genbank: BC031870.2
• Species: Human

Sequence of Insert

gcggccgcgatggccaaggagaggggcctaataagccccagtgattttgc
ccagctgcaaaaatacatggaatactccaccaaaaaggtcagtgatgtcc
taaagctcttcgaggatggcgagatggctaaatatgtccaaggagatgcc
attgggtacgagggattccagcaattcctgaaaatctatctcgaagtgga
taatgttcccagacacctaagcctggcactgtttcaatcctttgagactg
gtcactgcttaaatgagacaaatgtgacaaaagatgtggtgtgtctcaat
gatgtttcctgctacttttcccttctggagggtggtcggccagaagacaa
gttagaattcaccttcaagctgtacgacacggacagaaatgggatcctgg
acagctcagaagtggacaaaattatcctacagatgatgcgagtggctgaa
tacctggattgggatgtgtctgagctgaggccgattcttcaggagatgat
gaaagagattgactatgatggcagtggctctgtctctcaagctgagtggg
tccgggctggggccaccaccgtgccactgctagtgctgctgggtctggag
atgactctgaaggacgacggacagcacatgtggaggcccaagaggttccc
cagaccagtctactgcaatctgtgcgagtcaagcattggtcttggcaaac
agggactgagctgtaacctctgtaagtacactgttcacgaccagtgtgcc
atgaaagccctgccttgtgaagtcagcacctatgccaagtctcggaagga
cattggtgtccaatcacatgtgtgggtgcgaggaggctgtgagtccgggc
gctgcgaccgctgtcagaaaaagatccggatctaccacagtctgaccggg
ctgcattgtgtatggtgccacctagagatccacgatgactgcctgcaagc
ggtgggccatgagtgtgactgtgggctgctccgggatcacatcctgcctc
catcttccatctatcccagtgtcctggcctctggaccggatcgtaaaaat
agcaaaacaagccagaagaccatggatgatttaaatttgagcacctctga
ggctctgcggattgaccctgttcctaacacccacccacttctcgtctttg
tcaatcctaagagtggcgggaagcaggggcaaagggtgctctggaagttc
cagtatatattaaaccctcgacaggtgttcaacctcctaaaggatggtcc
tgagatagggctccgattattcaaggatgttcctgatagccggattttgg
tgtgtggtggagacggcacagtaggctggattctagagaccattgacaaa
gctaacttgccagttttgcctcctgttgctgtgttgcccctgggtactgg
aaatgatctggctcgatgcctaagatggggaggaggttatgaaggacaga
atctggcaaagatcctcaaggatttagagatgagtaaagtggtacatatg
gatcgatggtctgtggaggtgatacctcaacaaactgaagaaaaaagtga
cccagtcccctttcaaatcatcaataactacttctctattggcgtggatg
cctctattgctcatcgattctacatcatgcgagagaaatatccggagaag
ttcaacagcagaatgaagaacaagctatggtacttcgaatttgccacatc
tgaatccatcttctcaacatgcaaaaagctggaggagtctttgacagttg
agatctgtgggaaaccgctggatctgagcaacctgtccctagaaggcatc
gcagtgctaaacatccctagcatgcatggtggctccaacctctggggtga
taccaggagaccccatggggatatctatgggatcaaccaggccttaggtg
ctacagctaaagtcatcaccgaccctgatatcctgaaaacctgtgtacca
gacctaagtgacaagagactggaagtggttgggctggagggtgcaattga
gatgggccaaatctataccaagctcaagaatgctggacgtcggctggcca
agtgctctgagatcaccttccacaccacaaaaacccttcccatgcaaatt
gacggagaaccctggatgcagacgccctgtacaatcaagatcacccacaa
gaaccagatgcccatgctcatgggcccacccccccgctccaccaatttct
ttggcttcttgagctaagcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMAKERGLI
SPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLK
IYLEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEG
GRPEDKLEFTFKLYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRP
ILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMW
RPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTY
AKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHCVWCHLEIH
DDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDL
NLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFN
LLKDGPEIGLRLFKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAV
LPLGTGNDLARCLRWGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQ
TEEKSDPVPFQIINNYFSIGVDASIAHRFYIMREKYPEKFNSRMKNKLWY
FEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGG
SNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVG
LEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCT
IKITHKNQMPMLMGPPPRSTNFFGFLS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU39322
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee