cDNA Clone RAD23A

• Plasmid: pET156P-1-RAD23A TUBE
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-RAD23A TUBE
• Genbank: BC014026.2
• Tag: 6His
• Species: Human

Sequence of Insert

AGATCTGGTGGAGGTGTGACGGGCTCTGAGTATGAGACGATGCTGACGGA
GATCATGTCCATGGGCTATGAGCGAGAGCGGGTCGTGGCCGCCCTGAGAG
CCAGCTACAACAACCCCCACCGAGCCGTGGAGTATCTGCTCACGGGAATT
CCTGGGAGCCCCGAGCCGGAACACGGTTCTGGCGGCGGTGGATCTGGTGG
AGGTGTGACGGGCTCTGAGTATGAGACGATGCTGACGGAGATCATGTCCA
TGGGCTATGAGCGAGAGCGGGTCGTGGCCGCCCTGAGAGCCAGCTACAAC
AACCCCCACCGAGCCGTGGAGTATCTGCTCACGGGAATTCCTGGGAGCCC
CGAGCCGGAACACGGTTCTGGCGGCGGTGGATCTGGTGGAGGTGTGACGG
GCTCTGAGTATGAGACGATGCTGACGGAGATCATGTCCATGGGCTATGAG
CGAGAGCGGGTCGTGGCCGCCCTGAGAGCCAGCTACAACAACCCCCACCG
AGCCGTGGAGTATCTGCTCACGGGAATTCCTGGGAGCCCCGAGCCGGAAC
ACGGTTCTGGCGGCGGTGGATCTGGTGGAGGTGTGACGGGCTCTGAGTAT
GAGACGATGCTGACGGAGATCATGTCCATGGGCTATGAGCGAGAGCGGGT
CGTGGCCGCCCTGAGAGCCAGCTACAACAACCCCCACCGAGCCGTGGAGT
ATCTGCTCACGGGAATTCCTGGGAGCCCCGAGCCGGAACACGGTTCTTAA
GGCGGCGGTggatccACTAGTTCTAGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSGGGVTGSEYETMLTEIMSMGYERERVV
AALRASYNNPHRAVEYLLTGIPGSPEPEHGSGGGGSGGGVTGSEYETMLT
EIMSMGYERERVVAALRASYNNPHRAVEYLLTGIPGSPEPEHGSGGGGSG
GGVTGSEYETMLTEIMSMGYERERVVAALRASYNNPHRAVEYLLTGIPGS
PEPEHGSGGGGSGGGVTGSEYETMLTEIMSMGYERERVVAALRASYNNPH
RAVEYLLTGIPGSPEPEHGS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20166
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express Rad23A-based TUBE with N-terminal 6HIS tag (contains preScission protease site); ; Estimated MW of expressed protein : 28413 Daltons