cDNA Clone Rad1

• Plasmid: pAS2.6 yeast Rad1
• Parent Plasmid: pAS2.6
• Expressed: Gal4 DNA BD -Rad1
• Genbank: M15435.1
• Tag: GAL4 DNA-BD
• Species: Saccharomyces cerevisiae

Sequence of Insert

ggatccATGTCTCAGTTATTTTATCAGGGCGACTCTGATGATGAGCTCCAGGAGGAACTTACGAGGCAGACAACTCAAGCATCTCAAAGTTCTAAAATTAAAAATGAAGATGAACCCGACGACTCCAATCATCTTAATGAGGTGGAAAATGAAGATAGCAAAGTTTTAGATGACGATGCAGTGTTATACCCTCTTATACCTAATGAGCCAGATGACATAGAAACGTCTAAGCCCAATATTAACGATATTAGGCCAGTTGATATTCAATTGACTTTACCATTGCCGTTTCAGCAAAAAGTGGTAGAGAATTCATTAATTACTGAAGATGCATTAATCATAATGGGGAAAGGACTAGGATTGCTTGATATTGTGGCCAATTTATTGCATGTTTTAGCTACACCAACATCCATTAACGGACAACTAAAGCGAGCGCTCGTCCTAGTGTTGAATGCAAAACCTATAGATAATGTAAGAATCAAGGAGGCCTTAGAAGAGCTGTCGTGGTTCTCTAATACTGGGAAGGACGACGACGATACTGCTGTCGAGAGCGATGATGAACTTTTTGAAAGGCCTTTTAACGTAGTTACCGCGGACTCGCTGAGCATTGAAAAGAGAAGAAAGCTATATATTTCTGGCGGAATCTTGAGCATTACTTCTAGAATTCTCATTGTGGATCTCTTATCCGGCATTGTTCACCCAAATAGGGTTACGGGTATGCTGGTATTGAATGCAGACTCACTTCGACATAATTCGAATGAATCGTTTATATTAGAGATTTACAGGTCTAAAAATACTTGGGGTTTTATTAAAGCCTTTTCTGAAGCACCAGAGACGTTTGTCATGGAATTTTCACCCCTCAGGACGAAAATGAAAGAATTACGGCTAAAGAACGTTTTGCTATGGCCGAGGTTCAGGGTAGAGGTCTCTTCCTGTTTGAATGCCACTAATAAGACGTCACACAATAAAGTCATTGAAGTCAAGGTCTCCTTAACAAATTCCATGTCTCAGATACAGTTTGGCTTGATGGAATGTTTGAAAAAATGTATTGCTGAGTTAAGCAGAAAAAATCCTGAACTAGCTCTGGACTGGTGGAATATGGAAAATGTCCTGGATATAAACTTTATCAGGTCAATTGACTCGGTGATGGTGCCGAACTGGCACCGAATTTCTTATGAATCAAAACAACTGGTTAAGGATATAAGATTCCTACGCCACCTTTTAAAGATGCTCGTAACTTCAGACGCAGTTGACTTTTTTGGAGAGATTCAATTAAGTTTGGATGCCAATAAACCGTCAGTATCCCGAAAATACAGCGAATCACCGTGGCTATTGGTCGATGAGGCACAATTAGTCATATCGTATGCGAAGAAAAGAATATTTTACAAAAATGAATATACTTTAGAAGAAAATCCAAAATGGGAACAACTTATTCATATATTACATGATATTTCACATGAGAGAATGACCAATCACCTTCAGGGGCCTACTTTAGTTGCCTGTTCCGACAACCTTACATGTTTAGAACTAGCAAAGGTCTTGAATGCCTCAAACAAAAAAAGAGGAGTACGTCAAGTGCTTCTGAATAAATTGAAATGGTACAGAAAACAGAGGGAGGAAACGAAAAAATTGGTCAAAGAAGTGCAAAGTCAGGACACTTTTCCAGAGAATGCAACATTAAATGTAAGCTCGACATTTTCCAAAGAACAAGTGACCACGAAAAGAAGAAGGACAAGAGGTGCTTCACAAGTTGCGGCCGTTGAAAAGCTAAGGAATGCAGGTACCAATGTAGATATGGAGGTGGTTTTTGAGGATCATAAGTTATCTGAAGAAATTAAGAAGGGAAGCGGTGATGATTTGGATGACGGTCAGGAAGAAAATGCCGCAAACGATTCAAAGATTTTTGAAATACAAGAACAGGAAAATGAAATCCTTATCGATGATGGGGATGCTGAATTTGACAACGGAGAATTAGAGTATGTGGGCGACCTTCCGCAGCACATCACAACCCATTTCAATAAGGATTTATGGGCAGAACATTGCAACGAGTATGAATATGTTGATCGTCAGGACGAAATTTTAATCTCTACGTTTAAAAGTCTCAATGACAATTGCTCATTGCAGGAGATGATGCCCTCTTACATTATAATGTTTGAACCAGATATATCGTTTATCAGGCAGATTGAAGTTTATAAGGCCATAGTGAAGGATTTGCAACCAAAAGTATACTTCATGTACTACGGTGAAAGTATTGAAGAGCAAAGTCATTTGACTGCTATCAAGAGAGAGAAAGATGCTTTCACAAAGTTGATTAGAGAGAATGCAAATCTGTCCCATCACTTTGAAACGAATGAAGATCTTTCTCACTACAAAAATTTAGCTGAAAGGAAGTTGAAGCTTTCAAAATTACGAAAATCTAATACCAGAAATGCGGGTGGGCAGCAGGGATTCCATAATCTTACTCAGGATGTCGTCATTGTGGATACACGTGAGTTTAATGCCTCATTACCAGGCTTACTCTACCGATATGGCATAAGGGTTATTCCTTGTATGTTGACAGTCGGCGATTATGTGATAACTCCTGATATTTGTCTCGAAAGAAAATCGATTTCTGACTTAATTGGGTCATTACAGAATAACAGATTAGCCAACCAATGTAAAAAAATGTTAAAATACTATGCATATCCGACACTATTGATTGAGTTTGATGAAGGACAGTCGTTTTCTTTAGAACCTTTTAGTGAACGTAGAAATTATAAGAATAAAGACATATCAACTGTTCATCCTATATCAAGCAAGTTATCCCAGGATGAAATTCAGCTAAAACTAGCCAAATTAGTATTGCGGTTTCCCACTTTAAAGATTATATGGTCTTCCTCACCCCTGCAAACTGTAAATATAATCCTAGAGTTGAAATTAGGACGTGAGCAACCTGACCCTAGTAATGCAGTTATATTGGGAACGAATAAAGTTAGATCGGATTTTAATAGCACTGCAAAGGGCCTGAAGGATGGTGATAACGAGTCTAAATTCAAGAGACTGTTGAATGTTCCTGGAGTGTCAAAAATTGATTATTTCAATCTCCGCAAAAAGATCAAGAGCTTCAATAAGCTTCAAAAGCTTTCATGGAATGAGATTAATGAACTTATTAATGACGAAGATTTGACGGATAGAATATACTACTTCTTGAGAACAGAAAAAGAAGAACAAGAACAAGAGTCAACAGATGAAAATCTTGAATCTCCTGGTAAGACCACTGATGATAACGCTTTACATGATCATCATAATGATGTTCCTGAAGCACCTGTGTAAGCGGCCGC

Amino Acid Sequence

MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPVLQYPALTHMGSMSQLFYQGDSDDELQEELTRQTTQASQSSKIKNEDEPDDSNHLNEVENEDSKVLDDDAVLYPLIPNEPDDIETSKPNINDIRPVDIQLTLPLPFQQKVVENSLITEDALIIMGKGLGLLDIVANLLHVLATPTSINGQLKRALVLVLNAKPIDNVRIKEALEELSWFSNTGKDDDDTAVESDDELFERPFNVVTADSLSIEKRRKLYISGGILSITSRILIVDLLSGIVHPNRVTGMLVLNADSLRHNSNESFILEIYRSKNTWGFIKAFSEAPETFVMEFSPLRTKMKELRLKNVLLWPRFRVEVSSCLNATNKTSHNKVIEVKVSLTNSMSQIQFGLMECLKKCIAELSRKNPELALDWWNMENVLDINFIRSIDSVMVPNWHRISYESKQLVKDIRFLRHLLKMLVTSDAVDFFGEIQLSLDANKPSVSRKYSESPWLLVDEAQLVISYAKKRIFYKNEYTLEENPKWEQLIHILHDISHERMTNHLQGPTLVACSDNLTCLELAKVLNASNKKRGVRQVLLNKLKWYRKQREETKKLVKEVQSQDTFPENATLNVSSTFSKEQVTTKRRRTRGASQVAAVEKLRNAGTNVDMEVVFEDHKLSEEIKKGSGDDLDDGQEENAANDSKIFEIQEQENEILIDDGDAEFDNGELEYVGDLPQHITTHFNKDLWAEHCNEYEYVDRQDEILISTFKSLNDNCSLQEMMPSYIIMFEPDISFIRQIEVYKAIVKDLQPKVYFMYYGESIEEQSHLTAIKREKDAFTKLIRENANLSHHFETNEDLSHYKNLAERKLKLSKLRKSNTRNAGGQQGFHNLTQDVVIVDTREFNASLPGLLYRYGIRVIPCMLTVGDYVITPDICLERKSISDLIGSLQNNRLANQCKKMLKYYAYPTLLIEFDEGQSFSLEPFSERRNYKNKDISTVHPISSKLSQDEIQLKLAKLVLRFPTLKIIWSSSPLQTVNIILELKLGREQPDPSNAVILGTNKVRSDFNSTAKGLKDGDNESKFKRLLNVPGVSKIDYFNLRKKIKSFNKLQKLSWNEINELINDEDLTDRIYYFLRTEKEEQEQESTDENLESPGKTTDDNALHDHHNDVPEAPV*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU45080
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Unstable at 37 degrees. Grow at 30 or less.