cDNA Clone SIK3 (Long)

• Synonyms: qsk
• Plasmid: pEBG6P SIK3 delta 1-59
• Parent Plasmid: pEBG6P
• Expressed: GST SIK3 delta 1-59
• Genbank: NM_025164 covers residues 864 – 1369
• Tag: GST
• Species: Human

Sequence of Insert

ggatcCATGCCCGCCCGTATCGGCTACTACGAGATCGACCGCACCATCGG
CAAGGGCAACTTCGCGGTGGTCAAGCGGGCCACGCACCTCGTCACCAAGG
CCAAGGTTGCTATCAAGATCATAGATAAGACCCAGCTGGATGAAGAAAAC
TTGAAGAAGATTTTCCGGGAAGTTCAAATTATGAAGATGCTTTGCCACCC
CCATATCATCAGGCTCTACCAGGTTATGGAGACAGAACGGATGATTTATC
TGGTGACAGAATATGCTAGTGGAGGGGAAATATTTGACCACCTGGTGGCC
CATGGTAGAATGGCAGAAAAGGAGGCACGTCGGAAGTTCAAACAGATCGT
CACAGCTGTCTATTTTTGTCACTGTCGGAACATTGTTCATCGTGATTTAA
AAGCTGAAAATTTACTTCTGGATGCCAATCTGAATATCAAAATAGCAGAT
TTTGGTTTCAGTAACCTCTTCACTCCTGGGCAGCTGCTGAAGACCTGGTG
TGGCAGCCCTCCCTATGCTGCACCTGAACTCTTTGAAGGAAAAGAATATG
ATGGGCCCAAAGTGGACATCTGGAGCCTTGGAGTTGTCCTCTACGTGCTT
GTGTGCGGTGCCCTGCCATTTGATGGAAGCACACTGCAGAATCTGCGGGC
CCGCGTGCTGAGTGGAAAGTTCCGCATCCCATTTTTTATGTCCACAGAAT
GTGAGCATTTGATCCGCCATATGTTGGTGTTAGATCCCAATAAGCGCCTC
TCCATGGAGCAGATCTGCAAGCACAAGTGGATGAAGCTAGGGGACGCCGA
TCCCAACTTTGACAGGTTAATAGCTGAATGCCAACAACTAAAGGAAGAAA
GACAGGTGGACCCCCTGAATGAGGATGTCCTCTTGGCCATGGAGGACATG
GGACTGGACAAAGAACAGACACTGCAGTCATTAAGATCAGATGCCTATGA
TCACTATAGTGCAATCTACAGCCTGCTGTGTGATCGACATAAGAGACATA
AAACCCTGCGTCTCGGAGCACTTCCTAGCATGCCCCGAGCCCTGGCCTTT
CAAGCACCAGTCAATATCCAGGCGGAGCAGGCAGGTACTGCTATGAACAT
CAGCGTTCCCCAGGTGCAGCTGATCAACCCAGAGAACCAAATTGTGGAGC
CGGATGGGACACTGAATTTGGACAGTGATGAGGGTGAAGAGCCTTCCCCT
GAAGCATTGGTGCGCTATTTGTCAATGAGGAGGCACACAGTGGGTGTGGC
TGACCCACGCACGGAAGTTATGGAAGATCTGCAGAAGCTCCTACCTGGCT
TTCCTGGAGTCAACCCCCAGGCTCCATTCCTGCAGGTGGCCCCTAATGTG
AACTTCATGCACAACCTGTTGCCTATGCAAAACTTGCAACCAACCGGGCA
ACTTGAGTACAAGGAGCAGTCTCTCCTACAGCCGCCCACGCTACAGCTGT
TGAATGGAATGGGCCCCCTTGGCCGGAGGGCATCAGATGGAGGAGCCAAC
ATCCAACTGCATGCCCAGCAGCTGCTGAAGCGCCCACGGGGACCCTCTCC
GCTTGTCACCATGACACCAGCAGTGCCAGCAGTTACCCCTGTGGACGAGG
AGAGCTCAGACGGGGAGCCAGACCAGGAAGCTGTGCAGAGGTACTTGGCA
AATAGGTCCAAAAGACATACACTGGCCATGACCAACCCTACAGCTGAGAT
CCCACCGGACCTACAACGGCAGCTAGGACAGCAGCCTTTCCGTTCCCGGG
TCTGGCCTCCTCACCTGGTACCTGATCAGCATCGCTCTACCTACAAGGAC
TCCAACACTCTGCACCTCCCTACGGAGCGTTTCTCCCCTGTGCGCCGGTT
CTCAGATGGGGCTGCGAGCATCCAGGCCTTCAAAGCTCACCTGGAAAAAA
TGGGCAACAACAGCAGCATCAAACAGCTGCAGCAGGAGTGTGAGCAGCTG
CAGAAGATGTACGGGGGGCAGATTGATGAAAGAACCCTGGAGAAGACCCA
GCAGCAGCATATGTTATACCAGCAGGAGCAGCACCATCAAATTCTCCAGC
AACAAATTCAAGACTCTATCTGTCCTCCTCAGCCATCTCCACCTCTTCAG
GCTGCATGTGAAAATCAGCCAGCCCTCCTTACCCATCAGCTCCAGAGGTT
AAGGATTCAGCCTTCAAGCCCACCCCCCAACCACCCCAACAACCATCTCT
TCAGGCAGCCCAGTAATAGTCCTCCCCCCATGAGCAGTGCCATGATCCAG
CCTCACGGGGCTGCATCTTCTTCCCAGTTTCAAGGCTTACCTTCCCGCAG
TGCAATCTTTCAGCAGCAACCTGAGAACTGTTCCTCTCCTCCCAACGTGG
CACTAACCTGCTTGGGTATGCAGCAGCCTGCTCAGTCACAGCAGGTCACC
ATCCAAGTCCAAGAGCCTGTTGACATGCTCAGCAACATGCCAGGCACAGC
TGCAGGCTCCAGTGGGCGCGGCATCTCCATCAGCCCCAGTGCTGGTCAGA
TGCAGATGCAGCACCGTACCAACCTGATGGCCACCCTCAGCTATGGGCAC
CGTCCCTTGTCCAAGCAGCTGAGTGCTGACAGTGCAGAGGCTCACAGCTT
GAACGTGAATCGGTTCTCCCCTGCTAACTACGACCAGGCGCATTTACACC
CCCATCTGTTTTCGGACCAGTCCCGGGGTTCCCCCAGCAGCTACAGCCCT
TCAACAGGAGTGGGGTTCTCTCCAACCCAAGCCCTGAAAGTCCCTCCACT
TGACCAATTCCCCACCTTCCCTCCCAGTGCACATCAGCAGCCGCCACACT
ATACCACGTCGGCACTACAGCAGGCCCTGCTGTCTCCCACGCCGCCAGAC
TATACAAGACACCAGCAGGTACCCCACATCCTTCAAGGACTGCTTTCTCC
CCGGCATTCGCTCACCGGCCACTCGGACATCCGGCTGCCCCCAACAGAGT
TTGCACAGCTCATTAAAAGGCAGCAGCAACAACGGCAGCAGCAGCAGCAA
CAGCAGCAACAGCAAGAATACCAGGAACTGTTCAGGCACATGAACCAAGG
GGATGCGGGGAGTCTGGCTCCCAGCCTTGGGGGACAGAGCATGACAGAGC
GCCAGGCTTTATCTTATCAAAATGCTGACTCTTATCACCATCACACCAGC
CCCCAGCATCTGCTACAAATCAGGGCACAAGAATGTGTCTCACAGGCTTC
CTCACCCACCCCGCCCCACGGGTATGCTCACCAGCCGGCACTGATGCATT
CAGAGAGCATGGAGGAGGACTGCTCGTGTGAGGGGGCCAAGGATGGCTTC
CAAGACAGTAAGAGTTCAAGTACATTGACCAAAGGTTGCCATGACAGCCC
TCTGCTCTTGAGTACCGGTGGACCTGGGGACCCTGAATCTTTGCTAGGAA
CTGTGAGTCATGCCCAAGAATTGGGGATACATCCCTATGGTCATCAGCCA
ACTGCTGCATTCAGTAAAAATAAGGTGCCCAGCAGAGAGCCTGTCATAGG
GAACTGCATGGATAGAAGTTCTCCAGGACAAGCAGTGGAGCTGCCGGATC
ACAATGGGCTCGGGTACCCAGCACGCCCCTCCGTCCATGAGCACCACAGG
CCCCGGGCCCTCCAGAGACACCACACGATCCAGAACAGCGACGATGCTTA
TGTACAGCTGGATAACTTGCCAGGAATGAGTCTCGTGGCTGGGAAAGCAC
TTAGCTCTGCCCGGATGTCGGATGCAGTTCTCAGTCAGTCTTCGCTCATG
GGCAGCCAGCAGTTTCAGGATGGGGAAAATGAGGAATGTGGGGCAAGCCT
GGGAGGTCATGAGCACCCAGACCTGAGTGATGGCAGCCAGCATTTAAACT
CCTCTTGCTATCCATCTACGTGTATTACAGACATTCTGCTCAGCTACAAG
CACCCCGAAGTCTCCTTCAGCATGGAGCAGGCAGGCGTGTAAAAGCCGAA
TTCCAGCACACTGGCGGCCGTTACTAGTggatcc

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMPARIGYYEIDRT
IGKGNFAVVKRATHLVTKAKVAIKIIDKTQLDEENLKKIFREVQIMKMLC
HPHIIRLYQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARRKFKQ
IVTAVYFCHCRNIVHRDLKAENLLLDANLNIKIADFGFSNLFTPGQLLKT
WCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTLQNL
RARVLSGKFRIPFFMSTECEHLIRHMLVLDPNKRLSMEQICKHKWMKLGD
ADPNFDRLIAECQQLKEERQVDPLNEDVLLAMEDMGLDKEQTLQSLRSDA
YDHYSAIYSLLCDRHKRHKTLRLGALPSMPRALAFQAPVNIQAEQAGTAM
NISVPQVQLINPENQIVEPDGTLNLDSDEGEEPSPEALVRYLSMRRHTVG
VADPRTEVMEDLQKLLPGFPGVNPQAPFLQVAPNVNFMHNLLPMQNLQPT
GQLEYKEQSLLQPPTLQLLNGMGPLGRRASDGGANIQLHAQQLLKRPRGP
SPLVTMTPAVPAVTPVDEESSDGEPDQEAVQRYLANRSKRHTLAMTNPTA
EIPPDLQRQLGQQPFRSRVWPPHLVPDQHRSTYKDSNTLHLPTERFSPVR
RFSDGAASIQAFKAHLEKMGNNSSIKQLQQECEQLQKMYGGQIDERTLEK
TQQQHMLYQQEQHHQILQQQIQDSICPPQPSPPLQAACENQPALLTHQLQ
RLRIQPSSPPPNHPNNHLFRQPSNSPPPMSSAMIQPHGAASSSQFQGLPS
RSAIFQQQPENCSSPPNVALTCLGMQQPAQSQQVTIQVQEPVDMLSNMPG
TAAGSSGRGISISPSAGQMQMQHRTNLMATLSYGHRPLSKQLSADSAEAH
SLNVNRFSPANYDQAHLHPHLFSDQSRGSPSSYSPSTGVGFSPTQALKVP
PLDQFPTFPPSAHQQPPHYTTSALQQALLSPTPPDYTRHQQVPHILQGLL
SPRHSLTGHSDIRLPPTEFAQLIKRQQQQRQQQQQQQQQQEYQELFRHMN
QGDAGSLAPSLGGQSMTERQALSYQNADSYHHHTSPQHLLQIRAQECVSQ
ASSPTPPHGYAHQPALMHSESMEEDCSCEGAKDGFQDSKSSSTLTKGCHD
SPLLLSTGGPGDPESLLGTVSHAQELGIHPYGHQPTAAFSKNKVPSREPV
IGNCMDRSSPGQAVELPDHNGLGYPARPSVHEHHRPRALQRHHTIQNSDD
AYVQLDNLPGMSLVAGKALSSARMSDAVLSQSSLMGSQQFQDGENEECGA
SLGGHEHPDLSDGSQHLNSSCYPSTCITDILLSYKHPEVSFSMEQAGV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU43752
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: The sequence of this clone refers to an older reference sequence with an additional 59 amino acids at the N terminus.