cDNA Clone Dia2 (yeast)

• Plasmid: pFastbacDual DAC TEV yeast Dia2 G199-S732(end)
• Parent Plasmid: pFastbacDual DAC TEV
• Expressed: DAC TEV yeast Dia2 G199-S732(end)
• Genbank: NM_001183499.1 GI:296148081
• Tag: DAC
• Species: Yeast

Sequence of Insert

GGATCCGGTAGTACCAAGAAAACTGATTTAGTTGGCAATTTGCCGATAGAAATACTACCTATCATATTCCAAAGGTTTACCACTAAGGAGCTTGTTACGTTGTCGCTAGTTTGTAACAAATGGAGGGACAAAATTTTGTATCATTTAGACTGCTTCCAAGAATTCAACTTGGCACCAATAAATTTTAAAAATTTTGTAAAGTTTATGGATTTTTTACAACAGAATTTCACCAGGACGTATAGAAAATATATTCTTTCACAAGTAAAAGTCAGTTCTAGAATCACTTCGGAAGAGTTACGAATAACTCAATTATTATTCAGTAAAATGCCGAAATGTATAAACATCGAACGGCTTATACTGTCAATGCCAACACTAACTACAACACAAATCTTTAAGTTAATGGTTAGAGGAGGCACAGATTTTTTTACAAGGCTGCTGGAATTATCTCTCATGATAACTTATAGGCCTGATAAACAACATGAATTGGAGATACTGCAGACTTGTCCTCTGTTGAAAAAAATAGAATTAATTTTTGTCAATTCACTGGTACCAATTTTCGACGGAAATAATAGCGTTGGGAGGGATGGAAGTTTCAATGTGATGGCCCGTCATACTAATATGCAGATATCCACTGCGGATAATGATGAACAGGGAATAGTAGAAGAAAAGGTGATTTATAGTGAGCTAGAGAAGATAACATTGATATGTGATAAGAAGAAAATTAAGAATTTTCCACTTTGTCGTGCTCTATTAAGAGGCCAGTTTCCTCTATTGCAGAAACTGACGATAACTGGTGTAACATTTCCTATGAATAATCAAGACATAATGAATTTTCAATGGCTATTGAACTTCCCAGACCTAAAAGAACTATGGATAGAAGATAACGACAACTGTGAACTCAGTAAGTTTTTGCAATTGTTAAAATTTTCGAATGTCTGGAAAAACTTGGAAAAGTTAACCTTTAGAGAGAACAAGCTATATCCAATTGTTAATTTGGATGAAGATCAGCCTGTTACGAATGACGACGAGGTGCCATCCATGCTATTTTACAAGGAAAATCTCCAAAACCTGGAAAAGTTGGATCTAATGGGGACATCGATAAGTGGTTCTGCGTTGACTAGATTATGCGAGCAGGAATACTTGGATGGTCGGAAATTAAGAAGTTTAAACATTGGGAATTGCCCCAACATCCAGTTCCCAAATAATCACGCTCATACAGCAAGGATGATACTGGATGTGAATGCAGTTTTGAAGAGACTCTCCAAGTTGGAAGAAATAAATTTATCGCATCTCAGCTCCTTGAATGATAGTACGATGAAGTCATTCATTATTAATGTACCATTTTTGGAAAATTTAAAGAGGCTTGACATTTCGCACAATTTTGAAATTACAGGAATATCAATATATGAATTTCTAAAAAAATTTCAGATGGATCATGACAACGAAGCGGGAGGTCAACCACTGGCATATTTAAATATTGACGGATGTTCCCAAGTGTCTCATATCACAGTTAATATGATCCGAGCGCAGAACTTAGTTACTCAAGTGGATTGTGTTTATGAAAGAGATGTATGGAGAAAATTTGGAATTAATTCATATTCATACTCATAGCGGCCGC

Amino Acid Sequence

MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIG
QAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRG
INLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDA
DGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDN
SLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKL
LTWGFRFFETVNPENLYFQGGSGSTKKTDLVGNLPIEILPIIFQRFTTKE
LVTLSLVCNKWRDKILYHLDCFQEFNLAPINFKNFVKFMDFLQQNFTRTY
RKYILSQVKVSSRITSEELRITQLLFSKMPKCINIERLILSMPTLTTTQI
FKLMVRGGTDFFTRLLELSLMITYRPDKQHELEILQTCPLLKKIELIFVN
SLVPIFDGNNSVGRDGSFNVMARHTNMQISTADNDEQGIVEEKVIYSELE
KITLICDKKKIKNFPLCRALLRGQFPLLQKLTITGVTFPMNNQDIMNFQW
LLNFPDLKELWIEDNDNCELSKFLQLLKFSNVWKNLEKLTFRENKLYPIV
NLDEDQPVTNDDEVPSMLFYKENLQNLEKLDLMGTSISGSALTRLCEQEY
LDGRKLRSLNIGNCPNIQFPNNHAHTARMILDVNAVLKRLSKLEEINLSH
LSSLNDSTMKSFIINVPFLENLKRLDISHNFEITGISIYEFLKKFQMDHD
NEAGGQPLAYLNIDGCSQVSHITVNMIRAQNLVTQVDCVYERDVWRKFGI
NSYSYS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU49453
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental: DU22313