cDNA Clone Dia2 (yeast)/Skp1 (yeast)

• Plasmid: pFastbacDual DAC TEV yeast Dia2/untagged yeast Skp1
• Parent Plasmid: pFastbacDual DAC TEV
• Expressed: DAC TEV yeast Dia2
• Genbank: NM_001183499.1 GI:296148081
• Tag: DAC
• Species: Yeast

Sequence of Insert

GGATCCATGTCTTCCCCAGGGAATTCAGGCGTGGCTATAGATTCTACCGTTCTGAAAGCAATTGAATTAGGAACGAGGCTTTTTAAATCTGGAGAATATTTGCAAGCGAAAAGGATATTCACAAATGCTTTGAGAGTATGTGATTCTTATTCTCAGGAGCAGATTATGCGCATTAGGAACGCGTATCAATTAGATACTGCGAGACCGGATAACAAACGATTATATCATCCTAGATACATAAAGATATTGGATAACATCTGTGCGTGCTACGAAAAATTGAATGATTTGAAATCTTGTTTGGACGTATCACAAAGGTTACTTAAGTTAGAACCAGGTAATATCAAATGCTATATACGATGTACAAGGACGTTGATAAAATTAAAAGACTGGAAAAGGGCATATAAAACATGTTCTCGTGGACTGCAATTATGCAATAACGATAGCAATCATCTAAGACAACAAAAACAATTCATTAAAAATAACATGGTTCAAAAACAGGACGGCAAGAGAAGTTATATAGATCCATTAGAGGAGACCAAAATAGCAAAAAAAAAAAAGAATAATAATGTTCTAGAATCGTTACCAAAGAAGAAGATTAAAGGTAGTACCAAGAAAACTGATTTAGTTGGCAATTTGCCGATAGAAATACTACCTATCATATTCCAAAGGTTTACCACTAAGGAGCTTGTTACGTTGTCGCTAGTTTGTAACAAATGGAGGGACAAAATTTTGTATCATTTAGACTGCTTCCAAGAATTCAACTTGGCACCAATAAATTTTAAAAATTTTGTAAAGTTTATGGATTTTTTACAACAGAATTTCACCAGGACGTATAGAAAATATATTCTTTCACAAGTAAAAGTCAGTTCTAGAATCACTTCGGAAGAGTTACGAATAACTCAATTATTATTCAGTAAAATGCCGAAATGTATAAACATCGAACGGCTTATACTGTCAATGCCAACACTAACTACAACACAAATCTTTAAGTTAATGGTTAGAGGAGGCACAGATTTTTTTACAAGGCTGCTGGAATTATCTCTCATGATAACTTATAGGCCTGATAAACAACATGAATTGGAGATACTGCAGACTTGTCCTCTGTTGAAAAAAATAGAATTAATTTTTGTCAATTCACTGGTACCAATTTTCGACGGAAATAATAGCGTTGGGAGGGATGGAAGTTTCAATGTGATGGCCCGTCATACTAATATGCAGATATCCACTGCGGATAATGATGAACAGGGAATAGTAGAAGAAAAGGTGATTTATAGTGAGCTAGAGAAGATAACATTGATATGTGATAAGAAGAAAATTAAGAATTTTCCACTTTGTCGTGCTCTATTAAGAGGCCAGTTTCCTCTATTGCAGAAACTGACGATAACTGGTGTAACATTTCCTATGAATAATCAAGACATAATGAATTTTCAATGGCTATTGAACTTCCCAGACCTAAAAGAACTATGGATAGAAGATAACGACAACTGTGAACTCAGTAAGTTTTTGCAATTGTTAAAATTTTCGAATGTCTGGAAAAACTTGGAAAAGTTAACCTTTAGAGAGAACAAGCTATATCCAATTGTTAATTTGGATGAAGATCAGCCTGTTACGAATGACGACGAGGTGCCATCCATGCTATTTTACAAGGAAAATCTCCAAAACCTGGAAAAGTTGGATCTAATGGGGACATCGATAAGTGGTTCTGCGTTGACTAGATTATGCGAGCAGGAATACTTGGATGGTCGGAAATTAAGAAGTTTAAACATTGGGAATTGCCCCAACATCCAGTTCCCAAATAATCACGCTCATACAGCAAGGATGATACTGGATGTGAATGCAGTTTTGAAGAGACTCTCCAAGTTGGAAGAAATAAATTTATCGCATCTCAGCTCCTTGAATGATAGTACGATGAAGTCATTCATTATTAATGTACCATTTTTGGAAAATTTAAAGAGGCTTGACATTTCGCACAATTTTGAAATTACAGGAATATCAATATATGAATTTCTAAAAAAATTTCAGATGGATCATGACAACGAAGCGGGAGGTCAACCACTGGCATATTTAAATATTGACGGATGTTCCCAAGTGTCTCATATCACAGTTAATATGATCCGAGCGCAGAACTTAGTTACTCAAGTGGATTGTGTTTATGAAAGAGATGTATGGAGAAAATTTGGAATTAATTCATATTCATACTCATAGCGGCCGC

Amino Acid Sequence

MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIG
QAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRG
INLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDA
DGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDN
SLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKL
LTWGFRFFETVNPENLYFQGGSMSSPGNSGVAIDSTVLKAIELGTRLFKS
GEYLQAKRIFTNALRVCDSYSQEQIMRIRNAYQLDTARPDNKRLYHPRYI
KILDNICACYEKLNDLKSCLDVSQRLLKLEPGNIKCYIRCTRTLIKLKDW
KRAYKTCSRGLQLCNNDSNHLRQQKQFIKNNMVQKQDGKRSYIDPLEETK
IAKKKKNNNVLESLPKKKIKGSTKKTDLVGNLPIEILPIIFQRFTTKELV
TLSLVCNKWRDKILYHLDCFQEFNLAPINFKNFVKFMDFLQQNFTRTYRK
YILSQVKVSSRITSEELRITQLLFSKMPKCINIERLILSMPTLTTTQIFK
LMVRGGTDFFTRLLELSLMITYRPDKQHELEILQTCPLLKKIELIFVNSL
VPIFDGNNSVGRDGSFNVMARHTNMQISTADNDEQGIVEEKVIYSELEKI
TLICDKKKIKNFPLCRALLRGQFPLLQKLTITGVTFPMNNQDIMNFQWLL
NFPDLKELWIEDNDNCELSKFLQLLKFSNVWKNLEKLTFRENKLYPIVNL
DEDQPVTNDDEVPSMLFYKENLQNLEKLDLMGTSISGSALTRLCEQEYLD
GRKLRSLNIGNCPNIQFPNNHAHTARMILDVNAVLKRLSKLEEINLSHLS
SLNDSTMKSFIINVPFLENLKRLDISHNFEITGISIYEFLKKFQMDHDNE
AGGQPLAYLNIDGCSQVSHITVNMIRAQNLVTQVDCVYERDVWRKFGINS
YSYS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU49480
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental: DU22313