cDNA Clone Dia2 (yeast)/Ctf4 (yeast)

• Plasmid: pETDuet GST TEV yeast Dia2 M1-L206/yeast Ctf4 T461-K927(end)
• Parent Plasmid: pETDuet GST TEV
• Expressed: GST TEV yeast Dia2 M1-L206
• Genbank: NM_001183499.1 GI:296148081 with silent change g15c to kill internal EcoRI site
• Tag: GST
• Species: Yeast

Sequence of Insert

GAATTCATGTCTTCCCCAGGCAATTCAGGCGTGGCTATAGATTCTACCGTTCTGAAAGCAATTGAATTAGGAACGAGGCTTTTTAAATCTGGAGAATATTTGCAAGCGAAAAGGATATTCACAAATGCTTTGAGAGTATGTGATTCTTATTCTCAGGAGCAGATTATGCGCATTAGGAACGCGTATCAATTAGATACTGCGAGACCGGATAACAAACGATTATATCATCCTAGATACATAAAGATATTGGATAACATCTGTGCGTGCTACGAAAAATTGAATGATTTGAAATCTTGTTTGGACGTATCACAAAGGTTACTTAAGTTAGAACCAGGTAATATCAAATGCTATATACGATGTACAAGGACGTTGATAAAATTAAAAGACTGGAAAAGGGCATATAAAACATGTTCTCGTGGACTGCAATTATGCAATAACGATAGCAATCATCTAAGACAACAAAAACAATTCATTAAAAATAACATGGTTCAAAAACAGGACGGCAAGAGAAGTTATATAGATCCATTAGAGGAGACCAAAATAGCAAAAAAAAAAAAGAATAATAATGTTCTAGAATCGTTACCAAAGAAGAAGATTAAAGGTAGTACCAAGAAAACTGATTTATAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGRSEFMSSPGNSGVAIDSTVLKAI
ELGTRLFKSGEYLQAKRIFTNALRVCDSYSQEQIMRIRNAYQLDTARPDN
KRLYHPRYIKILDNICACYEKLNDLKSCLDVSQRLLKLEPGNIKCYIRCT
RTLIKLKDWKRAYKTCSRGLQLCNNDSNHLRQQKQFIKNNMVQKQDGKRS
YIDPLEETKIAKKKKNNNVLESLPKKKIKGSTKKTDL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU49529
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental: DU25128