cDNA Clone DGK iota

• Plasmid: pGEX6P1 DGK Iota
• Parent Plasmid: pGEX6P1
• Expressed: GST-DGK iota
• Genbank: O75912.1
• Tag: GST
• Species: Human

Sequence of Insert

gcggccgcgATGGACGCTGCTGGACGTGGTTGCCACCTGCTCCCACTGCCTGCTGCCAGAGGCCCCGCCCGTGCTCCTGCTGCCGCTGCCGCTGCCGCTGCCTCGCCCCCTGGACCTTGCTCTGGAGCTGCTTGCGCTCCTTCCGCTGCCGCTGGTGCTGGAGCTATGAACCCATCCTCTAGCGCCGGCGAGGAAAAGGGCGCTACCGGTGGCTCATCGTCCTCTGGTTCTGGTGCTGGATCTTGCTGTCTGGGTGCTGAGGGAGGTGCTGATCCTCGTGGTGCTGGTTCCGCCGCTGCCGCTGGTGCCGCTGCCCTCGACGAGCCAGCTGCCGCTGGCCAGAAGGAGAAGGATGAAGCTTTGGAGGAAAAGTTGAGAAACCTGACTTTCCGTAAGCAGGTTTCTTACCGCAAGGCCATCAGCAGGGCTGGACTCCAACACCTGGCTCCAGCTCACCCTCTCTCCTTGCCAGTGGCTAACGGTCCAGCTAAGGAGCCGCGTGCCACACTGGACTGGTCTGAAAACGCTGTTAACGGCGAGCACCTGTGGCTCGAAACCAACGTGAGCGGAGATCTGTGCTACCTCGGCGAGGAAAACTGTCAGGTCCGCTTCGCCAAGTCCGCTCTGCGTCGCAAGTGCGCCGTTTGTAAGATCGTGGTCCACACCGCTTGCATCGAGCAACTCGAAAAGATCAACTTCCGCTGCAAGCCTACTTTCCGTGAGGGCGGATCCAGGTCCCCAAGGGAAAACTTCGTGAGGCACCACTGGGTCCACAGGAGACGTCAGGAGGGCAAGTGCAAGCAATGTGGAAAGGGTTTCCAGCAAAAGTTCTCATTCCACTCGAAGGAGATCGTGGCCATCTCTTGCAGCTGGTGTAAGCAGGCTTTCCACAACAAGGTCACATGCTTCATGTTGCACCACATCGAGGAACCATGCTCCCTGGGAGCTCACGCCGCTGTTATCGTGCCACCGACTTGGATCATCAAGGTGAAGAAGCCCCAGAACAGCCTGAAGGCCTCAAACCGTAAGAAGAAGCGTACATCATTCAAGAGAAAGGCTTCGAAGCGTGGCATGGAGCAAGAAAACAAGGGACGTCCCTTCGTGATCAAGCCCATCAGCTCACCTTTGATGAAGCCATTGCTGGTCTTCGTTAACCCTAAGTCCGGTGGCAACCAGGGTACTAAGGTCCTCCAAATGTTCATGTGGTACCTGAATCCACGGCAAGTCTTTGATCTTTCTCAGGAAGGGCCAAAAGATGCGCTTGAATTGTATAGGAAAGTACCAAATCTGCGAATTCTGGCCTGTGGTGGGGATGGAACGGTGGGCTGGATCCTTTCCATCCTGGATGAACTGCAGCTGAGCCCTCAGCCTCCTGTGGGGGTCCTTCCTCTGGGGACTGGGAATGACCTGGCTCGAACTCTCAACTGGGGAGGGGGCTACACTGATGAACCTGTTTCTAAGATCCTGTGTCAAGTGGAAGATGGGACAGTTGTACAGCTAGATCGCTGGAACCTCCATGTGGAAAGAAACCCCGACTTGCCTCCAGAAGAACTTGAAGATGGCGTATGTAAGCTCCCTCTGAATGTTTTCAATAACTACTTCAGCCTTGGATTTGATGCCCATGTCACACTGGAGTTCCATGAATCCAGAGAAGCAAATCCAGAGAAATTCAACAGTCGTTTTCGAAATAAAATGTTCTATGCAGGGGCAGCTTTTTCTGACTTCCTACAGAGAAGTTCTAGAGATCTATCCAAACATGTTAAAGTTGTTTGTGATGGAACAGATCTCACCCCAAAGATTCAGGAACTGAAGTTCCAGTGTATAGTATTTTTAAATATACCCAGATATTGTGCTGGCACAATGCCCTGGGGAAACCCAGGTGATCACCATGATTTCGAACCTCAGCGTCATGATGATGGTTATATTGAAGTCATTGGATTTACCATGGCCTCTTTGGCAGCCCTGCAAGTTGGGGGCCATGGAGAGAGGCTACACCAGTGTCGAGAAGTCATGCTTCTAACTTACAAATCCATCCCCATGCAAGTGGATGGGGAGCCCTGTAGGTTGGCCCCAGCTATGATTCGGATCTCCCTGAGGAATCAGGCCAACATGGTACAGAAGAGCAAGAGGAGAACATCCATGCCTTTACTCAATGATCCCCAGTCTGTCCCAGATCGTCTGAGGATCCGGGTGAACAAAATCAGTTTACAAGACTATGAAGGATTCCACTATGACAAGGAGAAACTCCGAGAAGCTTCTATTTCAGACTGGTTAAGAACCATTGCTGGGGAACTAGTGCAGTCATTTGGAGCGATACCTCTGGGTATTCTAGTTGTGCGTGGAGACTGTGATTTGGAGACTTGCCGTATGTACATAGACCGCCTACAGGAGGACCTACAGTCAGTTTCTTCTGGCTCCCAGAGAGTTCATTACCAGGACCATGAAACCTCCTTCCCCAGGGCTCTCTCAGCACAGAGGCTCTCTCCTCGGTGGTGCTTCCTAGATGACAGATCTCAGGAACATTTGCACTTTGTGATGGAGATTTCCCAAGATGAGATTTTTATTCTGGACCCAGATATGGTGGTGTCACAGCCGGCGGGGACACCTCCGGGCATGCCTGACCTGGTGGTGGAACAAGCCTCGGGGATCTCAGACTGGTGGAATCCTGCCCTGCGGAAACGCATGCTGAGTGACAGTGGGCTGGGGATGATAGCTCCCTATTATGAGGACTCAGATCTGAAAGATCTCAGCCACTCCCGCGTGCTACAGTCACCAGTCTCTTCAGAGGATCATGCAATTTTGCAGGCAGTAATAGCTGGTGATCTTATGAAGCTAATAGAAAGCTATAAAAATGGAGGCAGTCTGCTAATTCAGGGACCAGACCACTGTTCACTCCTTCACTACGCAGCTAAAACCGGCAACGGGGAGATTGTGAAATATATCCTTGACCACGGACCTTCCGAGTTATTGGATATGGCAGACAGTGAAACGGGTGAGACTGCACTGCACAAGGCTGCCTGCCAGCGGAACCGGGCTGTGTGCCAGCTTCTGGTGGATGCAGGAGCATCTCTGAGAAAGACGGACTCCAAGGGTAAGACACCTCAAGAAAGAGCACAGCAGGCTGGGGACCCAGACTTGGCTGCTTACCTAGAAAGCCGTCAGAACTATAAGGTCATTGGCCATGAGGACCTGGAAACTGCTGTTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMDAAGRGCHLLPLPAARGPARAPAAAAAAAASPPGPCSGAACAPSAAAGAGAMNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGSAAAAGAAALDEPAAAGQKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASISDWLRTIAGELVQSFGAIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU25701
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutation at E926