cDNA Clone DHX57

• Plasmid: pSC-B DHX57
• Parent Plasmid: pSC-B
• Genbank: NP_945314.1
• Species: Human

Sequence of Insert

GCTAGCATGAGTTCTTCAGTAAGAAGAAAAGGCAAGCCAGGCAAAGGAGGTGGAAAAGGGTCTTCTAGAGGAGGAAGAGGAGGCAGGAGTCACGCCAGTAAATCTCATGGGAGTGGTGGCGGTGGCGGTGGTGGTGGTGGTGGAGGTGGCGGCAACAGAAAGGCCTCCAGTAGAATATGGGATGATGGAGATGACTTTTGTATCTTCAGTGAATCAAGGCGCCCTTCCAGACCTAGCAACAGTAACATAAGCAAAGGAGAGTCACGCCCAAAATGGAAACCCAAAGCCAAAGTACCCCTTCAGACTCTACATATGACTTCTGAGAATCAAGAGAAAGTGAAAGCTCTTCTCCGAGACCTGCAAGAACAAGATGCTGATGCTGGATCTGAAAGAGGCCTTTCTGGGGAGGAGGAAGATGATGAGCCTGATTGCTGTAACGATGAGCGGTACTGGCCAGCTGGACAGGAACCTTCCCTCGTTCCCGACTTGGATCCTTTGGAATATGCTGGCTTAGCCTCAGTGGAGCCTTATGTTCCAGAATTTACAGTCTCCCCATTTGCAGTGCAAAAACTTTCCAGGTATGGTTTCAATACTGAACGCTGTCAAGCGGTCCTGAGGATGTGTGATGGAGATGTGGGAGCATCACTAGAGCATCTCCTTACCCAGTGTTTTTCAGAGACATTTGGAGAGAGGATGAAGATCTCTGAGGCAGTCAACCAGATAAGCTTGGATGAGTGTATGGAACAGCGACAGGAAGAGGCATTTGCTCTCAAGTCCATCTGTGGAGAAAAATTTATAGAAAGAATTCAGAACAGAGTCTGGACCATTGGGTTAGAACTGGAGTATCTGACAAGTAGATTCCGCAAATCCAAGCCAAAAGAAAGTACCAAAAATGTACAAGAGAATTCACTTGAAATCTGTAAATTTTACCTCAAAGGAAATTGTAAATTTGGATCAAAATGCAGATTCAAACATGAAGTGCCCCCAAATCAAATTGTTGGAAGAATAGAAAGAAGTGTAGATGATTCTCATCTTAATGCTATTGAAGATGCATCTTTTTTATATGAACTTGAAATTCGATTTTCTAAAGACCACAAATATCCCTACCAAGCTCCGCTCGTGGCATTTTATTCCACCAATGAGAACCTACCTCTGGCTTGTCGTTTACATATTTCTGAGTTTCTTTATGACAAGGCCTTGACATTTGCGGAAACTTCGGAACCTGTCGTATATTCTTTGATAACCCTTTTAGAGGAAGAGTCGGAAATAGTCAAGTTACTAACGAATACCCACCACAAGTATAGTGACCCTCCTGTGAACTTTCTGCCAGTACCCTCTAGGACCAGAATAAATAATCCTGCCTGTCATAAAACAGTGATTCCAAATAATTCTTTTGTTTCTAATCAAATTCCAGAAGTTGAAAAAGCATCAGAATCTGAGGAGTCAGATGAGGATGACGGTCCTGCACCTGTTATAGTAGAGAATGAAAGCTATGTGAACCTTAAGAAAAAGATTTCCAAAAGATATGACTGGCAGGCAAAGTCAGTACATGCTGAAAATGGTAAAATCTGCAAGCAGTTCCGAATGAAACAGGCTTCCAGACAGTTCCAGTCCATTCTGCAAGAGAGGCAATCACTCCCTGCTTGGGAAGAAAGAGAAACCATTCTTAACTTATTGCGTAAGCACCAGGTGGTTGTCATAAGTGGTATGACTGGATGTGGGAAAACCACACAAATTCCGCAGTTTATTCTGGATGATTCTCTGAGTGGACCACCTGAGAAGGTAGCCAACATCATCTGTACCCAACCCCGACGAATCTCTGCAATCTCTGTTGCTGAACGCGTTGCTAAAGAAAGAGCAGAGAGGGTGGGTCTGACCGTGGGATACCAGATTCGGTTAGAAAGTGTCAAGTCCTCAGCCACCAGACTGTTATACTGCACCACGGGAGTGCTGCTGAGAAGGCTAGAAGGAGATACAGCTCTACAAGGAGTTTCCCATATCATTGTTGATGAAGTTCATGAGAGGACAGAAGAAAGTGACTTCTTGCTGCTAGTTTTGAAGGACATTGTATCGCAGAGGCCAGGTCTTCAAGTTATTTTAATGAGTGCAACTCTAAACGCTGAGCTTTTTTCAGACTATTTTAATTCCTGCCCCGTTATTACTATACCAGGTCGTACATTTCCTGTTGATCAATTTTTTTTGGAAGATGCAATTGCTGTGACAAGGTATGTATTACAGGATGGGAGCCCATATATGCGGTCCATGAAACAGATTTCAAAGGAAAAGCTTAAAGCAAGGCGGAACAGAACTGCATTTGAAGAAGTGGAAGAAGACCTAAGGCTCTCCCTTCACCTCCAGGATCAGGATTCTGTCAAAGATGCAGTGCCAGATCAACAGTTAGATTTTAAGCAGCTCCTGGCCCGCTATAAAGGGGTTAGCAAGTCAGTCATCAAAACAATGTCCATCATGGATTTTGAAAAGGTGAATCTTGAATTAATAGAGGCCTTATTAGAGTGGATTGTGGATGGAAAGCACTCCTACCCTCCAGGTGCTATACTTGTATTTTTACCAGGACTAGCAGAAATCAAAATGCTTTATGAACAGCTACAGTCTAATTCTCTTTTCAACAACAGACGTAGTAATCGATGTGTTATTCACCCACTTCATTCATCTTTATCCAGTGAAGAGCAGCAGGCTGTGTTTGTAAAACCTCCTGCAGGAGTAACTGATGCCAGCAAAGGGATGGAAAGTCTAGAGGACACCTTTGTATCTCAAGCTAATGCTCTACAAAGGAAAGGCCGAGCAGGCCGTGTTGCATCTGGGGTCTGCTTCCATTTATTCACTAGCCATCACTACAATCACCAGCTTTTAAAACAACAGCTACCAGAAATACAAAGAGTGCCATTGGAACAGCTGTGTCTAAGAATTAAAATTTTAGAGATGTTTAGTGCTCATAATCTCCAGTCTGTGTTCTCTCGGCTCATTGAACCTCCACACACCGATTCTCTTCGTGCCTCAAAAATACGATTACGAGACTTAGGAGCATTAACTCCAGATGAAAGATTGACCCCTCTTGGGTATCATTTGGCCTCTCTGCCCGTGGATGTGAGAATTGGCAAACTAATGTTGTTTGGGTCTATCTTCCGCTGTTTGGATCCTGCTCTCACCATTGCTGCCAGTTTGGCTTTTAAGTCGCCGTTTGTATCTCCCTGGGATAAAAAAGAAGAAGCTAACCAGAAAAAGCTGGAATTTGCATTCGCAAACAGTGATTATCTGGCCCTTCTACAAGCGTATAAGGGATGGCAGCTAAGTACAAAAGAAGGCGTGCGTGCAAGTTATAATTACTGCAGACAAAACTTCTTGTCTGGAAGAGTTCTGCAGGAAATGGCCAGCCTCAAACGACAATTCACGGAACTGTTATCGGATATAGGGTTTGCAAGGGAAGGGCTCAGAGCAAGGGAAATTGAGAAAAGGGCCCAAGGAGGAGATGGTGTCTTAGATGCCACAGGAGAAGAGGCAAACTCAAATGCTGAGAACCCCAAGCTGATATCAGCAATGCTGTGTGCTGCTTTGTATCCAAATGTAGTGCAGGTGAAAAGCCCAGAAGGAAAATTTCAGAAGACCAGTACTGGAGCTGTCAGAATGCAACCAAAATCAGCTGAGTTGAAGTTTGTCACCAAGAACGATGGATATGTACACATTCACCCTTCATCAGTGAACTATCAGGTGAGACACTTTGACAGCCCCTACCTGTTGTACCACGAGAAGATAAAAACTAGTCGAGTATTCATCCGAGACTGCAGCATGGTGTCTGTGTACCCGCTGGTCTTGTTTGGAGGAGGCCAAGTGAATGTGCAGCTTCAAAGAGGAGAGTTCGTTGTCTCCCTGGATGATGGTTGGATCCGTTTTGTAGCTGCTTCCCATCAGGTGGCTGAACTGGTAAAGGAGCTTCGTTGTGAACTTGATCAGCTTCTCCAGGATAAAATTAAAAACCCAAGCATTGATCTGTGTACGTGTCCTCGAGGATCCCGGATCATCAGCACAATTGTGAAACTTGTCACCACACAAGCGGCCGC

Amino Acid Sequence

MSSSVRRKGKPGKGGGKGSSRGGRGGRSHASKSHGSGGGGGGGGGGGGGNRKASSRIWDDGDDFCIFSESRRPSRPSNSNISKGESRPKWKPKAKVPLQTLHMTSENQEKVKALLRDLQEQDADAGSERGLSGEEEDDEPDCCNDERYWPAGQEPSLVPDLDPLEYAGLASVEPYVPEFTVSPFAVQKLSRYGFNTERCQAVLRMCDGDVGASLEHLLTQCFSETFGERMKISEAVNQISLDECMEQRQEEAFALKSICGEKFIERIQNRVWTIGLELEYLTSRFRKSKPKESTKNVQENSLEICKFYLKGNCKFGSKCRFKHEVPPNQIVGRIERSVDDSHLNAIEDASFLYELEIRFSKDHKYPYQAPLVAFYSTNENLPLACRLHISEFLYDKALTFAETSEPVVYSLITLLEEESEIVKLLTNTHHKYSDPPVNFLPVPSRTRINNPACHKTVIPNNSFVSNQIPEVEKASESEESDEDDGPAPVIVENESYVNLKKKISKRYDWQAKSVHAENGKICKQFRMKQASRQFQSILQERQSLPAWEERETILNLLRKHQVVVISGMTGCGKTTQIPQFILDDSLSGPPEKVANIICTQPRRISAISVAERVAKERAERVGLTVGYQIRLESVKSSATRLLYCTTGVLLRRLEGDTALQGVSHIIVDEVHERTEESDFLLLVLKDIVSQRPGLQVILMSATLNAELFSDYFNSCPVITIPGRTFPVDQFFLEDAIAVTRYVLQDGSPYMRSMKQISKEKLKARRNRTAFEEVEEDLRLSLHLQDQDSVKDAVPDQQLDFKQLLARYKGVSKSVIKTMSIMDFEKVNLELIEALLEWIVDGKHSYPPGAILVFLPGLAEIKMLYEQLQSNSLFNNRRSNRCVIHPLHSSLSSEEQQAVFVKPPAGVTDASKGMESLEDTFVSQANALQRKGRAGRVASGVCFHLFTSHHYNHQLLKQQLPEIQRVPLEQLCLRIKILEMFSAHNLQSVFSRLIEPPHTDSLRASKIRLRDLGALTPDERLTPLGYHLASLPVDVRIGKLMLFGSIFRCLDPALTIAASLAFKSPFVSPWDKKEEANQKKLEFAFANSDYLALLQAYKGWQLSTKEGVRASYNYCRQNFLSGRVLQEMASLKRQFTELLSDIGFAREGLRAREIEKRAQGGDGVLDATGEEANSNAENPKLISAMLCAALYPNVVQVKSPEGKFQKTSTGAVRMQPKSAELKFVTKNDGYVHIHPSSVNYQVRHFDSPYLLYHEKIKTSRVFIRDCSMVSVYPLVLFGGGQVNVQLQRGEFVVSLDDGWIRFVAASHQVAELVKELRCELDQLLQDKIKNPSIDLCTCPRGSRIISTIVKLVTTQ

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU25801
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: No stop for c-terminal tagging; Has 2 silent mutations S1095, C1349; 1 non silent mutation to be corrected S587N