cDNA Clone DFRAY (Drosophila)

• Plasmid: pGEX6P-1 dFRAY D185A
• Parent Plasmid: pGEX6P1
• Expressed: GST-3C-dFRAY D185A
• Genbank: AAN13778
• Tag: GST
• Species: Drosophila Melanogaster

Sequence of Insert

GGATCCATGACCAGCATTCCGGCAAATCTGAGCAGCAATAATGTTGCCGGTGCAGCAACCCTGCCTGGTGCAGCCGCACCGCCTGAGAAATATACCTGGCCGAATAGCAAAGATGATTATGAACTGCGTGATGTGATTGGTGTTGGTGCAACCGCAGTTGTTCATGGTGCATATTGTATTCCGCGTAATGAAAAATGCGCCATCAAACGCATTAACCTGGAAAAATGGAATACCAGCATGGATGAACTGCTGAAAGAAATTCAGGCAATGAGCAGCTGTTTTCACGAAAATGTTGTTACCTATCACACCAGCTTTGTTGTTCGTGAAGAACTGTGGCTGGTTCTGCGTCTGCTGGAAGGTGGTAGCCTGCTGGATATTATCAAACACAAAATGCGTACCAGCAATTGCAAACAGGGTGTTTTTGATGAAGCAACCATTGCAACCGTTCTGAAAGAAGTGCTGAAAGGCCTGGAATATTTTCATAGCAATGGTCAGATTCATCGGGATATTAAAGCCGGTAATATTCTGATTGGTGATGATGGCACCATTCAGATTGCAGCTTTTGGTGTTAGCGCATGGCTGGCAACCGGTCGTGATCTGAGCCGTCAGAAAGTTCGTCATACCTTTGTTGGCACCCCGTGTTGGATGGCACCGGAAGTTATGGAACAGGATCATGGCTATGATTTCAAAGCAGATATTTGGAGCTTTGGCATTACCGCAATTGAAATGGCCACCGGCACCGCACCGTATCATAAATATCCGCCTATGAAAGTTCTGATGCTGACCCTGCAGAATGATCCGCCTACCCTGGATACCGGTGCAGATGATAAAGATCAGTATAAAGCCTATGGCAAAACCTTTCGCAAAATGATTGTTGAGTGTCTGCAGAAAGAACCGAGCAAACGTCCGACCGCAAGCGAGCTGCTGAAACATGCCTTTTTCAAAAAAGCCAAAGACCGCAAATACCTGACCCAGACCCTGCTGCAGAGCGGTCCGAGCATGGAAACCCGTGTTCATAAAGCAGCAAAACGTCAGCCTGGTGCCAGCGGTCGTCTGCATCGTACCGTTACCGGTGAATGGGTTTGGAGCAGCGAAGAAGAAGATAACGGCGGTAGCGCAACCGGTAGCGGCACCGGTGATCGTAAACATCCGAGCAGCGATAGCGATAGTGAAGATCGTCCGATGAACCGTCTGGAACGTGCAGATAGCAGCGATTCAGATCGCGAAGAACCGTCACCGGAAATTACCCATAGCGTTAGCAGCGCCACCGTTACACCGGGTGCTCCGGCAGCAGCTGGTGCGGGTGCAGCACAAGAACTGACCGCAGGTATTGCACAGCTGCCGCTGCCGAGCGAAGCAGCCGGTGAAGCACCTCCGGTTAATCTGGTGCTGCGTATGCGTAATCTGCGTCGTGAACTGCATGATATTCGTTTTGAATTTGTGGTGGGTAAAGATACCGCAGAAGGTATTGCCACCGAACTGGTTGATGCAGGTCTGGTGGATGCACTGGATACCCAGCCGATGGCAACCCATCTGGATCAACTGATTGCAGCAAGCGCAACCATGAAAACCATTACCTTTCAGCTGAGCAGCGGTGTTCAGCCAGGTGAAGTTCCGGATGAACGTAGTCTGGTTGGTTATGCACAGATTAGCATTACCGATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMTSIPANLSSNNVAGAATL
PGAAAPPEKYTWPNSKDDYELRDVIGVGATAVVHGAYCIPRNEKCAIKRI
NLEKWNTSMDELLKEIQAMSSCFHENVVTYHTSFVVREELWLVLRLLEGG
SLLDIIKHKMRTSNCKQGVFDEATIATVLKEVLKGLEYFHSNGQIHRDIK
AGNILIGDDGTIQIAAFGVSAWLATGRDLSRQKVRHTFVGTPCWMAPEVM
EQDHGYDFKADIWSFGITAIEMATGTAPYHKYPPMKVLMLTLQNDPPTLD
TGADDKDQYKAYGKTFRKMIVECLQKEPSKRPTASELLKHAFFKKAKDRK
YLTQTLLQSGPSMETRVHKAAKRQPGASGRLHRTVTGEWVWSSEEEDNGG
SATGSGTGDRKHPSSDSDSEDRPMNRLERADSSDSDREEPSPEITHSVSS
ATVTPGAPAAAGAGAAQELTAGIAQLPLPSEAAGEAPPVNLVLRMRNLRR
ELHDIRFEFVVGKDTAEGIATELVDAGLVDALDTQPMATHLDQLIAASAT
MKTITFQLSSGVQPGEVPDERSLVGYAQISITD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU48534
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: D185A