cDNA Clone DHX57

• Synonyms: DDX57
• Plasmid: pcDNA5D FRT/TO DHX57 GFP
• Parent Plasmid: pcDNA5D FRT/TO C-GFP
• Expressed: DHX57 GFP
• Genbank: NM_198963.1
• Tag: GFP
• Species: Human

Sequence of Insert

GCTAGCATGAGTTCTTCAGTAAGAAGAAAAGGCAAGCCAGGCAAAGGAGGTGGAAAAGGGTCTTCTAGAGGAGGAAGAGGAGGCAGGAGTCACGCCAGTAAATCTCATGGGAGTGGTGGCGGTGGCGGTGGTGGTGGTGGTGGAGGTGGCGGCAACAGAAAGGCCTCCAGTAGAATATGGGATGATGGAGATGACTTTTGTATCTTCAGTGAATCAAGGCGCCCTTCCAGACCTAGCAACAGTAACATAAGCAAAGGAGAGTCACGCCCAAAATGGAAACCCAAAGCCAAAGTACCCCTTCAGACTCTACATATGACTTCTGAGAATCAAGAGAAAGTGAAAGCTCTTCTCCGAGACCTGCAAGAACAAGATGCTGATGCTGGATCTGAAAGAGGCCTTTCTGGGGAGGAGGAAGATGATGAGCCTGATTGCTGTAACGATGAGCGGTACTGGCCAGCTGGACAGGAACCTTCCCTCGTTCCCGACTTGGATCCTTTGGAATATGCTGGCTTAGCCTCAGTGGAGCCTTATGTTCCAGAATTTACAGTCTCCCCATTTGCAGTGCAAAAACTTTCCAGGTATGGTTTCAATACTGAACGCTGTCAAGCGGTCCTGAGGATGTGTGATGGAGATGTGGGAGCATCACTAGAGCATCTCCTTACCCAGTGTTTTTCAGAGACATTTGGAGAGAGGATGAAGATCTCTGAGGCAGTCAACCAGATAAGCTTGGATGAGTGTATGGAACAGCGACAGGAAGAGGCATTTGCTCTCAAGTCCATCTGTGGAGAAAAATTTATAGAAAGAATTCAGAACAGAGTCTGGACCATTGGGTTAGAACTGGAGTATCTGACAAGTAGATTCCGCAAATCCAAGCCAAAAGAAAGTACCAAAAATGTACAAGAGAATTCACTTGAAATCTGTAAATTTTACCTCAAAGGAAATTGTAAATTTGGATCAAAATGCAGATTCAAACATGAAGTGCCCCCAAATCAAATTGTTGGAAGAATAGAAAGAAGTGTAGATGATTCTCATCTTAATGCTATTGAAGATGCATCTTTTTTATATGAACTTGAAATTCGATTTTCTAAAGACCACAAATATCCCTACCAAGCTCCGCTCGTGGCATTTTATTCCACCAATGAGAACCTACCTCTGGCTTGTCGTTTACATATTTCTGAGTTTCTTTATGACAAGGCCTTGACATTTGCGGAAACTTCGGAACCTGTCGTATATTCTTTGATAACCCTTTTAGAGGAAGAGTCGGAAATAGTCAAGTTACTAACGAATACCCACCACAAGTATAGTGACCCTCCTGTGAACTTTCTGCCAGTACCCTCTAGGACCAGAATAAATAATCCTGCCTGTCATAAAACAGTGATTCCAAATAATTCTTTTGTTTCTAATCAAATTCCAGAAGTTGAAAAAGCATCAGAATCTGAGGAGTCAGATGAGGATGACGGTCCTGCACCTGTTATAGTAGAGAATGAAAGCTATGTGAACCTTAAGAAAAAGATTTCCAAAAGATATGACTGGCAGGCAAAGTCAGTACATGCTGAAAATGGTAAAATCTGCAAGCAGTTCCGAATGAAACAGGCTTCCAGACAGTTCCAGTCCATTCTGCAAGAGAGGCAATCACTCCCTGCTTGGGAAGAAAGAGAAACCATTCTTAACTTATTGCGTAAGCACCAGGTGGTTGTCATAAGTGGTATGACTGGATGTGGGAAAACCACACAAATTCCGCAGTTTATTCTGGATGATTCTCTGAATGGACCACCTGAGAAGGTAGCCAACATCATCTGTACCCAACCCCGACGAATCTCTGCAATCTCTGTTGCTGAACGCGTTGCTAAAGAAAGAGCAGAGAGGGTGGGTCTGACCGTGGGATACCAGATTCGGTTAGAAAGTGTCAAGTCCTCAGCCACCAGACTGTTATACTGCACCACGGGAGTGCTGCTGAGAAGGCTAGAAGGAGATACAGCTCTACAAGGAGTTTCCCATATCATTGTTGATGAAGTTCATGAGAGGACAGAAGAAAGTGACTTCTTGCTGCTAGTTTTGAAGGACATTGTATCGCAGAGGCCAGGTCTTCAAGTTATTTTAATGAGTGCAACTCTAAACGCTGAGCTTTTTTCAGACTATTTTAATTCCTGCCCCGTTATTACTATACCAGGTCGTACATTTCCTGTTGATCAATTTTTTTTGGAAGATGCAATTGCTGTGACAAGGTATGTATTACAGGATGGGAGCCCATATATGCGGTCCATGAAACAGATTTCAAAGGAAAAGCTTAAAGCAAGGCGGAACAGAACTGCATTTGAAGAAGTGGAAGAAGACCTAAGGCTCTCCCTTCACCTCCAGGATCAGGATTCTGTCAAAGATGCAGTGCCAGATCAACAGTTAGATTTTAAGCAGCTCCTGGCCCGCTATAAAGGGGTTAGCAAGTCAGTCATCAAAACAATGTCCATCATGGATTTTGAAAAGGTGAATCTTGAATTAATAGAGGCCTTATTAGAGTGGATTGTGGATGGAAAGCACTCCTACCCTCCAGGTGCTATACTTGTATTTTTACCAGGACTAGCAGAAATCAAAATGCTTTATGAACAGCTACAGTCTAATTCTCTTTTCAACAACAGACGTAGTAATCGATGTGTTATTCACCCACTTCATTCATCTTTATCCAGTGAAGAGCAGCAGGCTGTGTTTGTAAAACCTCCTGCAGGAGTAACTAAGATTATAATTTCCACCAACATTGCTGAGACATCCATAACCATCGATGATGTTGTCTATGTTATCGATTCTGGGAAAATGAAAGAAAAGAGATATGATGCCAGCAAAGGGATGGAAAGTCTAGAGGACACCTTTGTATCTCAAGCTAATGCTCTACAAAGGAAAGGCCGAGCAGGCCGTGTTGCATCTGGGGTCTGCTTCCATTTATTCACTAGCCATCACTACAATCACCAGCTTTTAAAACAACAGCTACCAGAAATACAAAGAGTGCCATTGGAACAGCTGTGTCTAAGAATTAAAATTTTAGAGATGTTTAGTGCTCATAATCTCCAGTCTGTGTTCTCTCGGCTCATTGAACCTCCACACACCGATTCTCTTCGTGCCTCAAAAATACGATTACGAGACTTAGGAGCATTAACTCCAGATGAAAGATTGACCCCTCTTGGGTATCATTTGGCCTCTCTGCCCGTGGATGTGAGAATTGGCAAACTAATGTTGTTTGGGTCTATCTTCCGCTGTTTGGATCCTGCTCTCACCATTGCTGCCAGTTTGGCTTTTAAGTCGCCGTTTGTATCTCCCTGGGATAAAAAAGAAGAAGCTAACCAGAAAAAGCTGGAATTTGCATTCGCAAACAGTGATTATCTGGCCCTTCTACAAGCGTATAAGGGATGGCAGCTAAGTACAAAAGAAGGCGTGCGTGCAAGTTATAATTACTGCAGACAAAACTTCTTGTCTGGAAGAGTTCTGCAGGAAATGGCCAGCCTCAAACGACAATTCACGGAACTGTTATCGGATATAGGGTTTGCAAGGGAAGGGCTCAGAGCAAGGGAAATTGAGAAAAGGGCCCAAGGAGGAGATGGTGTCTTAGATGCCACAGGAGAAGAGGCAAACTCAAATGCTGAGAACCCCAAGCTGATATCAGCAATGCTGTGTGCTGCTTTGTATCCAAATGTAGTGCAGGTGAAAAGCCCAGAAGGAAAATTTCAGAAGACCAGTACTGGAGCTGTCAGAATGCAACCAAAATCAGCTGAGTTGAAGTTTGTCACCAAGAACGATGGATATGTACACATTCACCCTTCATCAGTGAACTATCAGGTGAGACACTTTGACAGCCCCTACCTGTTGTACCACGAGAAGATAAAAACTAGTCGAGTATTCATCCGAGACTGCAGCATGGTGTCTGTGTACCCGCTGGTCTTGTTTGGAGGAGGCCAAGTGAATGTGCAGCTTCAAAGAGGAGAGTTCGTTGTCTCCCTGGATGATGGTTGGATCCGTTTTGTAGCTGCTTCCCATCAGGTGGCTGAACTGGTAAAGGAGCTTCGTTGTGAACTTGATCAGCTTCTCCAGGATAAAATTAAAAACCCAAGCATTGATCTGTGTACGTGTCCTCGAGGATCCCGGATCATCAGCACAATTGTGAAACTTGTCACCACACAAGCGGCCGC

Amino Acid Sequence

ASMSSSVRRKGKPGKGGGKGSSRGGRGGRSHASKSHGSGGGGGGGGGGGGGNRKASSRIWDDGDDFCIFSESRRPSRPSNSNISKGESRPKWKPKAKVPLQTLHMTSENQEKVKALLRDLQEQDADAGSERGLSGEEEDDEPDCCNDERYWPAGQEPSLVPDLDPLEYAGLASVEPYVPEFTVSPFAVQKLSRYGFNTERCQAVLRMCDGDVGASLEHLLTQCFSETFGERMKISEAVNQISLDECMEQRQEEAFALKSICGEKFIERIQNRVWTIGLELEYLTSRFRKSKPKESTKNVQENSLEICKFYLKGNCKFGSKCRFKHEVPPNQIVGRIERSVDDSHLNAIEDASFLYELEIRFSKDHKYPYQAPLVAFYSTNENLPLACRLHISEFLYDKALTFAETSEPVVYSLITLLEEESEIVKLLTNTHHKYSDPPVNFLPVPSRTRINNPACHKTVIPNNSFVSNQIPEVEKASESEESDEDDGPAPVIVENESYVNLKKKISKRYDWQAKSVHAENGKICKQFRMKQASRQFQSILQERQSLPAWEERETILNLLRKHQVVVISGMTGCGKTTQIPQFILDDSLNGPPEKVANIICTQPRRISAISVAERVAKERAERVGLTVGYQIRLESVKSSATRLLYCTTGVLLRRLEGDTALQGVSHIIVDEVHERTEESDFLLLVLKDIVSQRPGLQVILMSATLNAELFSDYFNSCPVITIPGRTFPVDQFFLEDAIAVTRYVLQDGSPYMRSMKQISKEKLKARRNRTAFEEVEEDLRLSLHLQDQDSVKDAVPDQQLDFKQLLARYKGVSKSVIKTMSIMDFEKVNLELIEALLEWIVDGKHSYPPGAILVFLPGLAEIKMLYEQLQSNSLFNNRRSNRCVIHPLHSSLSSEEQQAVFVKPPAGVTKIIISTNIAETSITIDDVVYVIDSGKMKEKRYDASKGMESLEDTFVSQANALQRKGRAGRVASGVCFHLFTSHHYNHQLLKQQLPEIQRVPLEQLCLRIKILEMFSAHNLQSVFSRLIEPPHTDSLRASKIRLRDLGALTPDERLTPLGYHLASLPVDVRIGKLMLFGSIFRCLDPALTIAASLAFKSPFVSPWDKKEEANQKKLEFAFANSDYLALLQAYKGWQLSTKEGVRASYNYCRQNFLSGRVLQEMASLKRQFTELLSDIGFAREGLRAREIEKRAQGGDGVLDATGEEANSNAENPKLISAMLCAALYPNVVQVKSPEGKFQKTSTGAVRMQPKSAELKFVTKNDGYVHIHPSSVNYQVRHFDSPYLLYHEKIKTSRVFIRDCSMVSVYPLVLFGGGQVNVQLQRGEFVVSLDDGWIRFVAASHQVAELVKELRCELDQLLQDKIKNPSIDLCTCPRGSRIISTIVKLVTTQAAAMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU25836
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Has 2 silent mutations as compared to NCBI ref seq S1095, C1349; Final construct pcDNA5-FRT-TO-DHX57-GFP