cDNA Clone DGK beta

• Plasmid: pFastbac DGK beta 1-804
• Parent Plasmid: pFastbac HTc
• Expressed: 6HIS DGK beta 1-804
• Genbank: NM_004080.2
• Tag: 6His
• Species: Human

Sequence of Insert

actagtATGACAAACCAGGAAAAATGGGCCCACCTCAGCCCTTCGGAATTTTCCCAACTTCAGAAATATGCTGAGTATTCTACAAAGAAATTAAAGGATG
TTCTTGAAGAATTCCATGGTAATGGTGTGCTTGCAAAGTATAATCCTGAAGGGAAACAAGACATTCTTAACCAAACAATAGATTTTGAAGGTTTCAAACT
ATTCATGAAGACATTCCTGGAAGCCGAGCTTCCTGATGATTTCACTGCACACCTTTTCATGTCATTTAGCAACAAGTTTCCTCATTCTAGTCCAATGGTA
AAAAGTAAGCCTGCTCTCCTATCAGGCGGTCTGAGAATGAATAAAGGTGCCATCACCCCTCCCCGAACTACTTCTCCTGCAAATACGTGTTCCCCAGAAG
TAATCCATCTGAAGGACATTGTCTGTTACCTGTCTCTGCTTGAAAGAGGAAGACCTGAGGATAAGCTTGAGTTTATGTTTCGCCTTTATGACACGGATGG
GAATGGCTTCCTGGACAGCTCGGAGCTAGAAAATATCATCAGTCAGATGATGCATGTTGCAGAATACCTTGAGTGGGATGTCACTGAACTTAATCCAATC
CTCCATGAAATGATGGAAGAAATTGACTATGATCATGATGGAACCGTGTCTCTGGAGGAATGGATTCAAGGAGGAATGACAACGATTCCACTTCTTGTGC
TCCTGGGCTTAGAAAATAACGTGAAGGATGATGGACAGCACGTGTGGCGACTGAAGCACTTTAACAAACCTGCCTATTGCAACCTTTGCCTGAACATGCT
GATTGGCGTGGGGAAGCAGGGCCTCTGCTGTTCCTTCTGCAAGTACACAGTCCATGAGCGCTGTGTGGCTCGAGCACCTCCCTCTTGCATCAAGACCTAT
GTGAAGTCCAAAAGGAACACTGATGTCATGCACCATTACTGGGTTGAAGGTAACTGCCCAACCAAGTGTGATAAGTGCCACAAAACTGTTAAATGTTACC
AGGGCCTGACAGGACTGCATTGTGTTTGGTGTCAGATCACACTGCATAATAAATGTGCTTCTCATCTAAAACCTGAATGTGACTGTGGACCTTTGAAGGA
CCATATTTTACCACCCACAACAATCTGTCCAGTGGTACTGCAGACTCTGCCCACTTCAGGAGTTTCAGTTCCTGAGGAAAGACAATCAACAGTGAAAAAG
GAAAAGAGTGGTTCCCAGCAGCCAAACAAAGTGATTGACAAGAATAAAATGCAAAGAGCCAACTCTGTTACTGTAGATGGACAAGGCCTGCAGGTCACTC
CTGTGCCTGGTACTCACCCACTTTTAGTTTTTGTGAACCCCAAAAGTGGTGGAAAACAAGGAGAACGAATTTACAGAAAATTCCAGTATCTATTAAATCC
TCGTCAGGTTTACAGTCTTTCTGGAAATGGACCAATGCCAGGGTTAAACTTTTTCCGTGATGTTCCTGACTTCAGAGTGTTAGCCTGTGGTGGAGATGGA
ACCGTGGGCTGGGTTTTGGATTGCATAGAAAAGGCCAATGTAGGCAAGCATCCTCCAGTTGCGATTCTGCCTCTTGGGACTGGCAATGATCTAGCAAGAT
GCCTGCGATGGGGAGGAGGTTACGAAGGTGAGAATCTGATGAAAATTCTAAAAGACATTGAAAACAGCACAGAAATCATGTTGGACAGGTGGAAGTTTGA
AGTCATACCTAATGACAAAGATGAGAAAGGAGACCCAGTGCCTTACAGTATCATCAATAATTACTTTTCCATTGGCGTGGATGCCTCCATTGCACACAGA
TTCCACATCATGAGAGAAAAACACCCAGAGAAATTCAACAGTAGAATGAAGAACAAATTTTGGTATTTTGAGTTTGGCACATCTGAAACTTTCTCAGCCA
CCTGCAAGAAGCTACATGAATCTGTAGAAATAGAATGTGATGGAGTACAGATAGATTTAATAAACATCTCTCTGGAAGGAATTGCTATTTTGAATATACC
AAGCATGCATGGAGGATCCAATCTTTGGGGAGAGTCTAAGAAAAGACGAAGCCATCGACGAATAGAGAAAAAAGGGTCTGACAAAAGGACCACCGTCACA
GATGCCAAAGAGTTGAAGTTTGCAAGTCAAGATCTCAGTGACCAGCTGCTGGAGGTGGTCGGCTTGGAAGGAGCCATGGAGATGGGGCAAATATACACAG
GCCTGAAAAGTGCTGGCCGGCGGCTGGCTCAGTGTTCCTGCGTGGTCATCAGGACGAGCAAGTCTCTGCCAATGCAAATTGATGGGGAGCCATGGATGCA
GACCCCATGCACAATAAAAATTACACACAAGAACCAAGCCCCAATGCTGATGGGCCCGCCTCCAAAAACCGGTTTATTCTGCTCCCTCGTCAAAAGGACA
AGAAACCGAAGCAAGGAAtaggtacc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDELTSMTNQEKWAH
LSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAKYNPEGKQDILNQTI
DFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKFPHSSPMVKSKPALLSG
GLRMNKGAITPPRTTSPANTCSPEVIHLKDIVCYLSLLERGRPEDKLEF
MFRLYDTDGNGFLDSSELENIISQMMHVAEYLEWDVTELNPILHEMMEE
IDYDHDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNK
PAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKR
NTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQITLHNKCAS
HLKPECDCGPLKDHILPPTTICPVVLQTLPTSGVSVPEERQSTVKKEKS
GSQQPNKVIDKNKMQRANSVTVDGQGLQVTPVPGTHPLLVFVNPKSGGK
QGERIYRKFQYLLNPRQVYSLSGNGPMPGLNFFRDVPDFRVLACGGDGT
VGWVLDCIEKANVGKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKIL
KDIENSTEIMLDRWKFEVIPNDKDEKGDPVPYSIINNYFSIGVDASIAH
RFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDG
VQIDLINISLEGIAILNIPSMHGGSNLWGESKKRRSHRRIEKKGSDKRT
TVTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCS
CVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPKTGLFCS
LVKRTRNRSKE

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU9153
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee