cDNA Clone SIK3 (Long)

• Synonyms: SIK3
• Plasmid: pCMV5-HA QSK T221A
• Parent Plasmid: pCMV5
• Expressed: HA-QSK T221A
• Genbank: NM_025164 covers residues 864 – 1369
• Tag: HA
• Species: Human

Sequence of Insert

gaattcGCCACCATGTACCCATACGATGTGCCAGATTACGCCGCGGCGGCGGCGGCGAGCGGAGCTGGCGGGGCTGCCGGGGCCGGGACTGGGGGAGCCG
GGCCCGCGGGCCGCCTGCTGCCTCCGCCCGCGCCGGGGTCCCCAGCCGCCCCCGCTGCCGTGTCCCCTGCGGCCGGCCAGCCGCGTCCCCCAGCCCCGGC
CTCCCGCGGACCCATGCCCGCCCGTATCGGCTACTACGAGATCGACCGCACCATCGGCAAGGGCAACTTCGCGGTGGTCAAGCGGGCCACGCACCTCGTC
ACCAAGGCCAAGGTTGCTATCAAGATCATAGATAAGACCCAGCTGGATGAAGAAAACTTGAAGAAGATTTTCCGGGAAGTTCAAATTATGAAGATGCTTT
GCCACCCCCATATCATCAGGCTCTACCAGGTTATGGAGACAGAACGGATGATTTATCTGGTGACAGAATATGCTAGTGGAGGGGAAATATTTGACCACCT
GGTGGCCCATGGTAGAATGGCAGAAAAGGAGGCACGTCGGAAGTTCAAACAGATCGTCACAGCTGTCTATTTTTGTCACTGTCGGAACATTGTTCATCGT
GATTTAAAAGCTGAAAATTTACTTCTGGATGCCAATCTGAATATCAAAATAGCAGATTTTGGTTTCAGTAACCTCTTCACTCCTGGGCAGCTGCTGAAGg
CCTGGTGTGGCAGCCCTCCCTATGCTGCACCTGAACTCTTTGAAGGAAAAGAATATGATGGGCCCAAAGTGGACATCTGGAGCCTTGGAGTTGTCCTCTA
CGTGCTTGTGTGCGGTGCCCTGCCATTTGATGGAAGCACACTGCAGAATCTGCGGGCCCGCGTGCTGAGTGGAAAGTTCCGCATCCCATTTTTTATGTCC
ACAGAATGTGAGCATTTGATCCGCCATATGTTGGTGTTAGATCCCAATAAGCGCCTCTCCATGGAGCAGATCTGCAAGCACAAGTGGATGAAGCTAGGGG
ACGCCGATCCCAACTTTGACAGGTTAATAGCTGAATGCCAACAACTAAAGGAAGAAAGACAGGTGGACCCCCTGAATGAGGATGTCCTCTTGGCCATGGA
GGACATGGGACTGGACAAAGAACAGACACTGCAGTCATTAAGATCAGATGCCTATGATCACTATAGTGCAATCTACAGCCTGCTGTGTGATCGACATAAG
AGACATAAAACCCTGCGTCTCGGAGCACTTCCTAGCATGCCCCGAGCCCTGGCCTTTCAAGCACCAGTCAATATCCAGGCGGAGCAGGCAGGTACTGCTA
TGAACATCAGCGTTCCCCAGGTGCAGCTGATCAACCCAGAGAACCAAATTGTGGAGCCGGATGGGACACTGAATTTGGACAGTGATGAGGGTGAAGAGCC
TTCCCCTGAAGCATTGGTGCGCTATTTGTCAATGAGGAGGCACACAGTGGGTGTGGCTGACCCACGCACGGAAGTTATGGAAGATCTGCAGAAGCTCCTA
CCTGGCTTTCCTGGAGTCAACCCCCAGGCTCCATTCCTGCAGGTGGCCCCTAATGTGAACTTCATGCACAACCTGTTGCCTATGCAAAACTTGCAACCAA
CCGGGCAACTTGAGTACAAGGAGCAGTCTCTCCTACAGCCGCCCACGCTACAGCTGTTGAATGGAATGGGCCCCCTTGGCCGGAGGGCATCAGATGGAGG
AGCCAACATCCAACTGCATGCCCAGCAGCTGCTGAAGCGCCCACGGGGACCCTCTCCGCTTGTCACCATGACACCAGCAGTGCCAGCAGTTACCCCTGTG
GACGAGGAGAGCTCAGACGGGGAGCCAGACCAGGAAGCTGTGCAGAGGTACTTGGCAAATAGGTCCAAAAGACATACACTGGCCATGACCAACCCTACAG
CTGAGATCCCACCGGACCTACAACGGCAGCTAGGACAGCAGCCTTTCCGTTCCCGGGTCTGGCCTCCTCACCTGGTACCTGATCAGCATCGCTCTACCTA
CAAGGACTCCAACACTCTGCACCTCCCTACGGAGCGTTTCTCCCCTGTGCGCCGGTTCTCAGATGGGGCTGCGAGCATCCAGGCCTTCAAAGCTCACCTG
GAAAAAATGGGCAACAACAGCAGCATCAAACAGCTGCAGCAGGAGTGTGAGCAGCTGCAGAAGATGTACGGGGGGCAGATTGATGAAAGAACCCTGGAGA
AGACCCAGCAGCAGCATATGTTATACCAGCAGGAGCAGCACCATCAAATTCTCCAGCAACAAATTCAAGACTCTATCTGTCCTCCTCAGCCATCTCCACC
TCTTCAGGCTGCATGTGAAAATCAGCCAGCCCTCCTTACCCATCAGCTCCAGAGGTTAAGGATTCAGCCTTCAAGCCCACCCCCCAACCACCCCAACAAC
CATCTCTTCAGGCAGCCCAGTAATAGTCCTCCCCCCATGAGCAGTGCCATGATCCAGCCTCACGGGGCTGCATCTTCTTCCCAGTTTCAAGGCTTACCTT
CCCGCAGTGCAATCTTTCAGCAGCAACCTGAGAACTGTTCCTCTCCTCCCAACGTGGCACTAACCTGCTTGGGTATGCAGCAGCCTGCTCAGTCACAGCA
GGTCACCATCCAAGTCCAAGAGCCTGTTGACATGCTCAGCAACATGCCAGGCACAGCTGCAGGCTCCAGTGGGCGCGGCATCTCCATCAGCCCCAGTGCT
GGTCAGATGCAGATGCAGCACCGTACCAACCTGATGGCCACCCTCAGCTATGGGCACCGTCCCTTGTCCAAGCAGCTGAGTGCTGACAGTGCAGAGGCTC
ACAGCTTGAACGTGAATCGGTTCTCCCCTGCTAACTACGACCAGGCGCATTTACACCCCCATCTGTTTTCGGACCAGTCCCGGGGTTCCCCCAGCAGCTA
CAGCCCTTCAACAGGAGTGGGGTTCTCTCCAACCCAAGCCCTGAAAGTCCCTCCACTTGACCAATTCCCCACCTTCCCTCCCAGTGCACATCAGCAGCCG
CCACACTATACCACGTCGGCACTACAGCAGGCCCTGCTGTCTCCCACGCCGCCAGACTATACAAGACACCAGCAGGTACCCCACATCCTTCAAGGACTGC
TTTCTCCCCGGCATTCGCTCACCGGCCACTCGGACATCCGGCTGCCCCCAACAGAGTTTGCACAGCTCATTAAAAGGCAGCAGCAACAACGGCAGCAGCA
GCAGCAACAGCAGCAACAGCAAGAATACCAGGAACTGTTCAGGCACATGAACCAAGGGGATGCGGGGAGTCTGGCTCCCAGCCTTGGGGGACAGAGCATG
ACAGAGCGCCAGGCTTTATCTTATCAAAATGCTGACTCTTATCACCATCACACCAGCCCCCAGCATCTGCTACAAATCAGGGCACAAGAATGTGTCTCAC
AGGCTTCCTCACCCACCCCGCCCCACGGGTATGCTCACCAGCCGGCACTGATGCATTCAGAGAGCATGGAGGAGGACTGCTCGTGTGAGGGGGCCAAGGA
TGGCTTCCAAGACAGTAAGAGTTCAAGTACATTGACCAAAGGTTGCCATGACAGCCCTCTGCTCTTGAGTACCGGTGGACCTGGGGACCCTGAATCTTTG
CTAGGAACTGTGAGTCATGCCCAAGAATTGGGGATACATCCCTATGGTCATCAGCCAACTGCTGCATTCAGTAAAAATAAGGTGCCCAGCAGAGAGCCTG
TCATAGGGAACTGCATGGATAGAAGTTCTCCAGGACAAGCAGTGGAGCTGCCGGATCACAATGGGCTCGGGTACCCAGCACGCCCCTCCGTCCATGAGCA
CCACAGGCCCCGGGCCCTCCAGAGACACCACACGATCCAGAACAGCGACGATGCTTATGTACAGCTGGATAACTTGCCAGGAATGAGTCTCGTGGCTGGG
AAAGCACTTAGCTCTGCCCGGATGTCGGATGCAGTTCTCAGTCAGTCTTCGCTCATGGGCAGCCAGCAGTTTCAGGATGGGGAAAATGAGGAATGTGGGG
CAAGCCTGGGAGGTCATGAGCACCCAGACCTGAGTGATGGCAGCCAGCATTTAAACTCCTCTTGCTATCCATCTACGTGTATTACAGACATTCTGCTCAG
CTACAAGCACCCCGAAGTCTCCTTCAGCATGGAGCAGGCAGGCGTGTAAAAGCCgaattc

Amino Acid Sequence

MYPYDVPDYAAAAAASGAGGAAGAGTGGAGPAGRLLPPPAPGSPAAPAAVSPAAGQPRPPAPASRGPMPARIGYYEIDRTIGKGNFAVVKRATHLVTKAK
VAIKIIDKTQLDEENLKKIFREVQIMKMLCHPHIIRLYQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYFCHCRNIVHRDLKA
ENLLLDANLNIKIADFGFSNLFTPGQLLKAWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTLQNLRARVLSGKFRIPFFMSTECE
HLIRHMLVLDPNKRLSMEQICKHKWMKLGDADPNFDRLIAECQQLKEERQVDPLNEDVLLAMEDMGLDKEQTLQSLRSDAYDHYSAIYSLLCDRHKRHKT
LRLGALPSMPRALAFQAPVNIQAEQAGTAMNISVPQVQLINPENQIVEPDGTLNLDSDEGEEPSPEALVRYLSMRRHTVGVADPRTEVMEDLQKLLPGFP
GVNPQAPFLQVAPNVNFMHNLLPMQNLQPTGQLEYKEQSLLQPPTLQLLNGMGPLGRRASDGGANIQLHAQQLLKRPRGPSPLVTMTPAVPAVTPVDEES
SDGEPDQEAVQRYLANRSKRHTLAMTNPTAEIPPDLQRQLGQQPFRSRVWPPHLVPDQHRSTYKDSNTLHLPTERFSPVRRFSDGAASIQAFKAHLEKMG
NNSSIKQLQQECEQLQKMYGGQIDERTLEKTQQQHMLYQQEQHHQILQQQIQDSICPPQPSPPLQAACENQPALLTHQLQRLRIQPSSPPPNHPNNHLFR
QPSNSPPPMSSAMIQPHGAASSSQFQGLPSRSAIFQQQPENCSSPPNVALTCLGMQQPAQSQQVTIQVQEPVDMLSNMPGTAAGSSGRGISISPSAGQMQ
MQHRTNLMATLSYGHRPLSKQLSADSAEAHSLNVNRFSPANYDQAHLHPHLFSDQSRGSPSSYSPSTGVGFSPTQALKVPPLDQFPTFPPSAHQQPPHYT
TSALQQALLSPTPPDYTRHQQVPHILQGLLSPRHSLTGHSDIRLPPTEFAQLIKRQQQQRQQQQQQQQQQEYQELFRHMNQGDAGSLAPSLGGQSMTERQ
ALSYQNADSYHHHTSPQHLLQIRAQECVSQASSPTPPHGYAHQPALMHSESMEEDCSCEGAKDGFQDSKSSSTLTKGCHDSPLLLSTGGPGDPESLLGTV
SHAQELGIHPYGHQPTAAFSKNKVPSREPVIGNCMDRSSPGQAVELPDHNGLGYPARPSVHEHHRPRALQRHHTIQNSDDAYVQLDNLPGMSLVAGKALS
SARMSDAVLSQSSLMGSQQFQDGENEECGASLGGHEHPDLSDGSQHLNSSCYPSTCITDILLSYKHPEVSFSMEQAGV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1291
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: The sequence of this clone refers to an older reference sequence with an additional 59 amino acids at the N terminus.