cDNA Clone SIK3

• Plasmid: pEX-GST-SIK3 isoform 1 T142I
• Parent Plasmid: pEX-GST
• Expressed: SIK3 T142I-GST
• Genbank: NM_025164.4 → NP_079440.3
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGCGGCGGCGGCGGCGAGCGGAGCTGGCGGGGCTGCCGGGGC
CGGGACTGGGGGAGCCGGGCCCGCGGGCCGCCTGCTGCCTCCGCCCGCGC
CGGGGTCCCCAGCCGCCCCCGCTGCCGTGTCCCCTGCGGCCGGCCAGCCG
CGTCCCCCAGCCCCGGCCTCCCGCGGACCCATGCCCGCCCGTATCGGCTA
CTACGAGATCGACCGCACCATCGGCAAGGGCAACTTCGCGGTGGTCAAGC
GGGCCACGCACCTCGTCACCAAGGCCAAGGTTGCTATCAAGATCATAGAT
AAGACCCAGCTGGATGAAGAAAACTTGAAGAAGATTTTCCGGGAAGTTCA
AATTATGAAGATGCTTTGCCACCCCCATATCATCAGGCTCTACCAGGTTA
TGGAGACAGAACGGATGATTTATCTGGTGAtAGAATATGCTAGTGGAGGG
GAAATATTTGACCACCTGGTGGCCCATGGTAGAATGGCAGAAAAGGAGGC
ACGTCGGAAGTTCAAACAGATCGTCACAGCTGTCTATTTTTGTCACTGTC
GGAACATTGTTCATCGTGATTTAAAAGCTGAAAATTTACTTCTGGATGCC
AATCTGAATATCAAAATAGCAGATTTTGGTTTCAGTAACCTCTTCACTCC
TGGGCAGCTGCTGAAGACCTGGTGTGGCAGCCCTCCCTATGCTGCACCTG
AACTCTTTGAAGGAAAAGAATATGATGGGCCCAAAGTGGACATCTGGAGC
CTTGGAGTTGTCCTCTACGTGCTTGTGTGCGGTGCCCTGCCATTTGATGG
AAGCACACTGCAGAATCTGCGGGCCCGCGTGCTGAGTGGAAAGTTCCGCA
TCCCATTTTTTATGTCCACAGAATGTGAGCATTTGATCCGCCATATGTTG
GTGTTAGATCCCAATAAGCGCCTCTCCATGGAGCAGATCTGCAAGCACAA
GTGGATGAAGCTAGGGGACGCCGATCCCAACTTTGACAGGTTAATAGCTG
AATGCCAACAACTAAAGGAAGAAAGACAGGTGGACCCCCTGAATGAGGAT
GTCCTCTTGGCCATGGAGGACATGGGACTGGACAAAGAACAGACACTGCA
GTCATTAAGATCAGATGCCTATGATCACTATAGTGCAATCTACAGCCTGC
TGTGTGATCGACATAAGAGACATAAAACCCTGCGTCTCGGAGCACTTCCT
AGCATGCCCCGAGCCCTGGCCTTTCAAGCACCAGTCAATATCCAGGCGGA
GCAGGCAGGTACTGCTATGAACATCAGCGTTCCCCAGGTGCAGCTGATCA
ACCCAGAGAACCAAATTGTGGAGCCGGATGGGACACTGAATTTGGACAGT
GATGAGGGTGAAGAGCCTTCCCCTGAAGCATTGGTGCGCTATTTGTCAAT
GAGGAGGCACACAGTGGGTGTGGCTGACCCACGCACGGAAGTTATGGAAG
ATCTGCAGAAGCTCCTACCTGGCTTTCCTGGAGTCAACCCCCAGGCTCCA
TTCCTGCAGGTGGCCCCTAATGTGAACTTCATGCACAACCTGTTGCCTAT
GCAAAACTTGCAACCAACCGGGCAACTTGAGTACAAGGAGCAGTCTCTCC
TACAGCCGCCCACGCTACAGCTGTTGAATGGAATGGGCCCCCTTGGCCGG
AGGGCATCAGATGGAGGAGCCAACATCCAACTGCATGCCCAGCAGCTGCT
GAAGCGCCCACGGGGACCCTCTCCGCTTGTCACCATGACACCAGCAGTGC
CAGCAGTTACCCCTGTGGACGAGGAGAGCTCAGACGGGGAGCCAGACCAG
GAAGCTGTGCAGAGCTCTACCTACAAGGACTCCAACACTCTGCACCTCCC
TACGGAGCGTTTCTCCCCTGTGCGCCGGTTCTCAGATGGGGCTGCGAGCA
TCCAGGCCTTCAAAGCTCACCTGGAAAAAATGGGCAACAACAGCAGCATC
AAACAGCTGCAGCAGGAGTGTGAGCAGCTGCAGAAGATGTACGGGGGGCA
GATTGATGAAAGAACCCTGGAGAAGACCCAGCAGCAGCATATGTTATACC
AGCAGGAGCAGCACCATCAAATTCTCCAGCAACAAATTCAAGACTCTATC
TGTCCTCCTCAGCCATCTCCACCTCTTCAGGCTGCATGTGAAAATCAGCC
AGCCCTCCTTACCCATCAGCTCCAGAGGTTAAGGATTCAGCCTTCAAGCC
CACCCCCCAACCACCCCAACAACCATCTCTTCAGGCAGCCCAGTAATAGT
CCTCCCCCCATGAGCAGTGCCATGATCCAGCCTCACGGGGCTGCATCTTC
TTCCCAGTTTCAAGGCTTACCTTCCCGCAGTGCAATCTTTCAGCAGCAAC
CTGAGAACTGTTCCTCTCCTCCCAACGTGGCACTAACCTGCTTGGGTATG
CAGCAGCCTGCTCAGTCACAGCAGGTCACCATCCAAGTCCAAGAGCCTGT
TGACATGCTCAGCAACATGCCAGGCACAGCTGCAGGCTCCAGTGGGCGCG
GCATCTCCATCAGCCCCAGTGCTGGTCAGATGCAGATGCAGCACCGTACC
AACCTGATGGCCACCCTCAGCTATGGGCACCGTCCCTTGTCCAAGCAGCT
GAGTGCTGACAGTGCAGAGGCTCACAGCTTGAACGTGAATCGGTTCTCCC
CTGCTAACTACGACCAGGCGCATTTACACCCCCATCTGTTTTCGGACCAG
TCCCGGGGTTCCCCCAGCAGCTACAGCCCTTCAACAGGAGTGGGGTTCTC
TCCAACCCAAGCCCTGAAAGTCCCTCCACTTGACCAATTCCCCACCTTCC
CTCCCAGTGCACATCAGCAGCCGCCACACTATACCACGTCGGCACTACAG
CAGGCCCTGCTGTCTCCCACGCCGCCAGACTATACAAGACACCAGCAGGT
ACCCCACATCCTTCAAGGACTGCTTTCTCCCCGGCATTCGCTCACCGGCC
ACTCGGACATCCGGCTGCCCCCAACAGAGTTTGCACAGCTCATTAAAAGG
CAGCAGCAACAACGGCAGCAGCAGCAGCAACAGCAGCAACAGCAAGAATA
CCAGGAACTGTTCAGGCACATGAACCAAGGGGATGCGGGGAGTCTGGCTC
CCAGCCTTGGGGGACAGAGCATGACAGAGCGCCAGGCTTTATCTTATCAA
AATGCTGACTCTTATCACCATCACACCAGCCCCCAGCATCTGCTACAAAT
CAGGGCACAAGAATGTGTCTCACAGGCTTCCTCACCCACCCCGCCCCACG
GGTATGCTCACCAGCCGGCACTGATGCATTCAGAGAGCATGGAGGAGGAC
TGCTCGTGTGAGGGGGCCAAGGATGGCTTCCAAGACAGTAAGAGTTCAAG
TACATTGACCAAAGGTTGCCATGACAGCCCTCTGCTCTTGAGTACCGGTG
GACCTGGGGACCCTGAATCTTTGCTAGGAACTGTGAGTCATGCCCAAGAA
TTGGGGATACATCCCTATGGTCATCAGCCAACTGCTGCATTCAGTAAAAA
TAAGGTGCCCAGCAGAGAGCCTGTCATAGGGAACTGCATGGATAGAAGTT
CTCCAGGACAAGCAGTGGAGCTGCCGGATCACAATGGGCTCGGGTACCCA
GCACGCCCCTCCGTCCATGAGCACCACAGGCCCCGGGCCCTCCAGAGACA
CCACACGATCCAGAACAGCGACGATGCTTATGTACAGCTGGATAACTTGC
CAGGAATGAGTCTCGTGGCTGGGAAAGCACTTAGCTCTGCCCGGATGTCG
GATGCAGTTCTCAGTCAGTCTTCGCTCATGGGCAGCCAGCAGTTTCAGGA
TGGGGAAAATGAGGAATGTGGGGCAAGCCTGGGAGGTCATGAGCACCCAG
ACCTGAGTGATGGCAGCCAGCATTTAAACTCCTCTTGCTATCCATCTACG
TGTATTACAGACATTCTGCTCAGCTACAAGCACCCCGAAGTCTCCTTCAG
CATGGAGCAGGCAGGCGTGgcggccgc

Amino Acid Sequence

MAAAAASGAGGAAGAGTGGAGPAGRLLPPPAPGSPAAPAAVSPAAGQPRP
PAPASRGPMPARIGYYEIDRTIGKGNFAVVKRATHLVTKAKVAIKIIDKT
QLDEENLKKIFREVQIMKMLCHPHIIRLYQVMETERMIYLVIEYASGGEI
FDHLVAHGRMAEKEARRKFKQIVTAVYFCHCRNIVHRDLKAENLLLDANL
NIKIADFGFSNLFTPGQLLKTWCGSPPYAAPELFEGKEYDGPKVDIWSLG
VVLYVLVCGALPFDGSTLQNLRARVLSGKFRIPFFMSTECEHLIRHMLVL
DPNKRLSMEQICKHKWMKLGDADPNFDRLIAECQQLKEERQVDPLNEDVL
LAMEDMGLDKEQTLQSLRSDAYDHYSAIYSLLCDRHKRHKTLRLGALPSM
PRALAFQAPVNIQAEQAGTAMNISVPQVQLINPENQIVEPDGTLNLDSDE
GEEPSPEALVRYLSMRRHTVGVADPRTEVMEDLQKLLPGFPGVNPQAPFL
QVAPNVNFMHNLLPMQNLQPTGQLEYKEQSLLQPPTLQLLNGMGPLGRRA
SDGGANIQLHAQQLLKRPRGPSPLVTMTPAVPAVTPVDEESSDGEPDQEA
VQSSTYKDSNTLHLPTERFSPVRRFSDGAASIQAFKAHLEKMGNNSSIKQ
LQQECEQLQKMYGGQIDERTLEKTQQQHMLYQQEQHHQILQQQIQDSICP
PQPSPPLQAACENQPALLTHQLQRLRIQPSSPPPNHPNNHLFRQPSNSPP
PMSSAMIQPHGAASSSQFQGLPSRSAIFQQQPENCSSPPNVALTCLGMQQ
PAQSQQVTIQVQEPVDMLSNMPGTAAGSSGRGISISPSAGQMQMQHRTNL
MATLSYGHRPLSKQLSADSAEAHSLNVNRFSPANYDQAHLHPHLFSDQSR
GSPSSYSPSTGVGFSPTQALKVPPLDQFPTFPPSAHQQPPHYTTSALQQA
LLSPTPPDYTRHQQVPHILQGLLSPRHSLTGHSDIRLPPTEFAQLIKRQQ
QQRQQQQQQQQQQEYQELFRHMNQGDAGSLAPSLGGQSMTERQALSYQNA
DSYHHHTSPQHLLQIRAQECVSQASSPTPPHGYAHQPALMHSESMEEDCS
CEGAKDGFQDSKSSSTLTKGCHDSPLLLSTGGPGDPESLLGTVSHAQELG
IHPYGHQPTAAFSKNKVPSREPVIGNCMDRSSPGQAVELPDHNGLGYPAR
PSVHEHHRPRALQRHHTIQNSDDAYVQLDNLPGMSLVAGKALSSARMSDA
VLSQSSLMGSQQFQDGENEECGASLGGHEHPDLSDGSQHLNSSCYPSTCI
TDILLSYKHPEVSFSMEQAGVAAALEVLFQGPLSRRSPILGYWKIKGLVQ
PTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKL
TQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDF
ETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYM
DPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDH
PPKSD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28774
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: isoform 1.
• Low Temp: No