Human Recombinant Protein - PKC epsilon (1 - 737)

Name: PKC epsilon (1 - 737)
Price per aliquot (100µg): £110.00
Long Name:
Protein Kinase C Epsilon
Synonyms:
PRKCE, PKCE, protein kinase C, epsilon
Species:
Human
Catalog Number (DU Number):
DU33642
Accession:
NM_005400.3
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Cobalt Agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM CaCl2, 0.1 mg/ml PtdSerine, 10 uM DAG, 10 mM DTT, 10 mM Magnesium Acetate
Enzymatic Substrate:
ERMRPRKRQGSVRRV Final concentration: 300 uM
Calculated molecular mass:
Mono-Isotopic Mass: 86, 989.48 daltons
Average Mass: 87, 045.42 daltons
Protein Activity:
Constitutively Active
Purity:
>85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical PI:
6.54
Native Sequence:
Amino acids M1 – P737 (end) of human PRKCE. Residue M29 of the fusion protein is equivalent to M1 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 – 23
Cloning Sites:
EcoR1 and Not1 sites of pFastBac.Hta

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